Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IPQ37_RS02300 Genome accession   NZ_CP065041
Coordinates   454825..455931 (+) Length   368 a.a.
NCBI ID   WP_237042629.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain AS-16-0963-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449825..460931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IPQ37_RS02270 (IPQ37_02270) - 450661..451254 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  IPQ37_RS02275 (IPQ37_02275) yggU 451356..451655 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  IPQ37_RS02280 (IPQ37_02280) - 451652..452209 (-) 558 WP_005297682.1 YggT family protein -
  IPQ37_RS02285 (IPQ37_02285) proC 452221..453039 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  IPQ37_RS02290 (IPQ37_02290) - 453055..453756 (-) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  IPQ37_RS02295 (IPQ37_02295) pilT 453779..454813 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  IPQ37_RS02300 (IPQ37_02300) pilU 454825..455931 (+) 1107 WP_237042629.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IPQ37_RS02305 (IPQ37_02305) ruvX 456259..456684 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  IPQ37_RS02310 (IPQ37_02310) - 456681..457244 (-) 564 WP_151170852.1 YqgE/AlgH family protein -
  IPQ37_RS02315 (IPQ37_02315) gshB 457398..458345 (-) 948 WP_005297697.1 glutathione synthase -
  IPQ37_RS02320 (IPQ37_02320) rsmE 458366..459097 (-) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IPQ37_RS02325 (IPQ37_02325) endA 459229..459933 (-) 705 WP_005297700.1 deoxyribonuclease I -
  IPQ37_RS02330 (IPQ37_02330) - 460064..460546 (-) 483 WP_036763215.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41301.46 Da        Isoelectric Point: 5.4197

>NTDB_id=506137 IPQ37_RS02300 WP_237042629.1 454825..455931(+) (pilU) [Photobacterium damselae subsp. damselae strain AS-16-0963-3]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDCTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=506137 IPQ37_RS02300 WP_237042629.1 454825..455931(+) (pilU) [Photobacterium damselae subsp. damselae strain AS-16-0963-3]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATTGTACAGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGCCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTAACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.605

96.196

0.573
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.691

93.207

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367