Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   Azoinq_RS10295 Genome accession   NZ_CP064782
Coordinates   2249703..2250131 (+) Length   142 a.a.
NCBI ID   WP_216129381.1    Uniprot ID   A0A975SL10
Organism   Azospira inquinata strain Azo-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2244703..2255131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Azoinq_RS10270 (Azoinq_10260) sucD 2245221..2246114 (+) 894 WP_216129391.1 succinate--CoA ligase subunit alpha -
  Azoinq_RS10275 (Azoinq_10265) - 2246184..2246906 (+) 723 WP_216129389.1 TerC family protein -
  Azoinq_RS10280 (Azoinq_10270) - 2246970..2247944 (+) 975 WP_216129387.1 ornithine cyclodeaminase family protein -
  Azoinq_RS10285 (Azoinq_10275) - 2248071..2248703 (+) 633 WP_216129385.1 FHA domain-containing protein -
  Azoinq_RS10290 (Azoinq_10280) - 2248738..2249511 (+) 774 WP_216129383.1 3',5'-cyclic-nucleotide phosphodiesterase -
  Azoinq_RS10295 (Azoinq_10285) pilA2 2249703..2250131 (+) 429 WP_216129381.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Azoinq_RS10300 (Azoinq_10290) tfpZ 2250132..2250965 (-) 834 WP_216129380.1 TfpX/TfpZ family type IV pilin accessory protein -
  Azoinq_RS10305 (Azoinq_10295) - 2251007..2251495 (-) 489 WP_216129378.1 histidine phosphatase family protein -
  Azoinq_RS10310 (Azoinq_10300) - 2251531..2253048 (-) 1518 WP_216129376.1 CHAD domain-containing protein -
  Azoinq_RS10315 (Azoinq_10305) - 2253060..2253746 (-) 687 WP_332460825.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14371.57 Da        Isoelectric Point: 7.5905

>NTDB_id=504730 Azoinq_RS10295 WP_216129381.1 2249703..2250131(+) (pilA2) [Azospira inquinata strain Azo-3]
MKSMQKGFTLIELMIVVAIIGILAALAIPAYSDYMKKSRFSEVQGIAEALKKDVALCAGDNTANPGTFTGCTNGALGIPP
KPTATTNTASVAVTDGVITGTATAAADGITTVLTPTLDASGSGVITWTNSGTCVNKGWCKAN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=504730 Azoinq_RS10295 WP_216129381.1 2249703..2250131(+) (pilA2) [Azospira inquinata strain Azo-3]
ATGAAATCTATGCAAAAAGGCTTTACCCTCATCGAACTGATGATCGTGGTGGCGATTATCGGCATTCTGGCTGCTCTGGC
CATTCCGGCCTATTCGGATTACATGAAGAAATCCCGCTTTTCTGAAGTTCAGGGCATTGCGGAAGCTTTGAAGAAGGATG
TGGCCTTGTGTGCCGGGGACAATACGGCTAATCCGGGGACGTTTACCGGTTGTACCAATGGAGCTTTGGGTATTCCTCCC
AAGCCCACCGCTACGACTAACACCGCCTCCGTTGCAGTGACAGATGGGGTGATTACCGGTACGGCGACAGCTGCTGCTGA
TGGTATCACTACGGTTTTAACTCCTACGTTGGATGCTTCTGGATCTGGTGTTATCACCTGGACTAATAGTGGTACGTGTG
TTAACAAAGGCTGGTGCAAGGCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

53.906

90.141

0.486
  pilA2 Legionella pneumophila strain ERS1305867

52.344

90.141

0.472
  pilA Ralstonia pseudosolanacearum GMI1000

42.537

94.366

0.401
  comP Acinetobacter baylyi ADP1

42.537

94.366

0.401
  pilA Haemophilus influenzae 86-028NP

39.695

92.254

0.366
  pilA Haemophilus influenzae Rd KW20

39.695

92.254

0.366