Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HWI32_RS06260 Genome accession   NZ_CP064568
Coordinates   1315311..1316378 (+) Length   355 a.a.
NCBI ID   WP_001831131.1    Uniprot ID   A0A9Q5JLH5
Organism   Staphylococcus epidermidis strain UMCG359     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1310311..1321378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI32_RS06230 (HWI32_06210) - 1310729..1312189 (+) 1461 WP_001832757.1 rhomboid family intramembrane serine protease -
  HWI32_RS06235 (HWI32_06215) - 1312173..1312373 (+) 201 WP_001831138.1 YqgQ family protein -
  HWI32_RS06240 (HWI32_06220) - 1312373..1313359 (+) 987 WP_001831113.1 ROK family glucokinase -
  HWI32_RS06245 (HWI32_06225) - 1313359..1313685 (+) 327 WP_001831234.1 MTH1187 family thiamine-binding protein -
  HWI32_RS06250 (HWI32_06230) - 1313685..1314308 (+) 624 WP_001831016.1 MBL fold metallo-hydrolase -
  HWI32_RS06255 (HWI32_06235) comGA 1314365..1315339 (+) 975 WP_001831109.1 competence type IV pilus ATPase ComGA Machinery gene
  HWI32_RS06260 (HWI32_06240) comGB 1315311..1316378 (+) 1068 WP_001831131.1 competence type IV pilus assembly protein ComGB Machinery gene
  HWI32_RS06265 (HWI32_06245) comGC 1316396..1316713 (+) 318 WP_001831271.1 competence type IV pilus major pilin ComGC Machinery gene
  HWI32_RS06270 (HWI32_06250) comGD 1316703..1317140 (+) 438 WP_001831025.1 competence type IV pilus minor pilin ComGD -
  HWI32_RS06275 (HWI32_06255) - 1317127..1317420 (+) 294 WP_001831116.1 hypothetical protein -
  HWI32_RS06280 (HWI32_06260) - 1317344..1317835 (+) 492 WP_002440043.1 competence type IV pilus minor pilin ComGF -
  HWI32_RS06285 (HWI32_06265) - 1318000..1318512 (+) 513 WP_002440040.1 shikimate kinase -
  HWI32_RS06290 (HWI32_06270) gcvT 1318730..1319821 (+) 1092 WP_001831107.1 glycine cleavage system aminomethyltransferase GcvT -
  HWI32_RS06295 (HWI32_06275) gcvPA 1319841..1321187 (+) 1347 WP_002456181.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 42379.68 Da        Isoelectric Point: 10.1702

>NTDB_id=503158 HWI32_RS06260 WP_001831131.1 1315311..1316378(+) (comGB) [Staphylococcus epidermidis strain UMCG359]
MKKLSINTFKYKRNKYLTEIQSIDLLQRLQQLLSHGFTLYQSFKFLNSYFKYKERTINKKIIQHLQNGATCYDILKIIGY
PELVLLQIKFAENYGNIEEALVDTVQYMKRNLKAKKRLIKTLQYPVALISIFLFILTILNITVIPQFQQLYETMNVKLST
FQNLLTLIITRLPKLTFIFIFISGIAFFITYKFYYYLPIEKKLKSILKIPMINTYYKIYRTYQLSNQLSLFYRNGTSLQQ
IVRIYRNEQDNDFLKFLGDYLFKEANKGLPLPVILMNLKCFQNDLIKFIEQGEKNGKLDIELKLYSQMLLQQFEEKVLKQ
TKFIQPIIFFILGIFIVSLYLVIMLPMFELMQTIK

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=503158 HWI32_RS06260 WP_001831131.1 1315311..1316378(+) (comGB) [Staphylococcus epidermidis strain UMCG359]
GTGAAGAAGTTGTCGATAAATACATTTAAATATAAGAGGAATAAATATCTTACTGAAATACAATCAATAGACTTACTACA
GAGATTACAACAGCTTTTAAGTCACGGATTCACTTTATATCAAAGTTTTAAATTTTTAAACTCCTATTTTAAATATAAAG
AGCGAACAATAAATAAAAAGATTATCCAACATCTACAAAACGGTGCTACATGTTATGATATTTTAAAAATAATAGGGTAT
CCAGAATTAGTTCTTCTTCAAATAAAATTTGCTGAAAACTACGGCAACATTGAGGAGGCTCTCGTTGATACTGTTCAATA
TATGAAAAGAAATCTGAAAGCTAAAAAACGACTCATCAAAACCTTACAATATCCTGTTGCATTAATTTCTATCTTCTTAT
TCATATTAACCATTTTAAATATAACTGTCATACCTCAATTTCAACAACTTTATGAGACTATGAATGTTAAATTATCAACA
TTTCAAAATCTACTAACTCTTATTATTACCCGTCTTCCCAAACTAACTTTCATTTTTATCTTTATTAGTGGTATAGCATT
TTTTATCACTTATAAATTCTACTATTATCTACCAATTGAGAAAAAGTTAAAATCTATTTTAAAAATCCCAATGATTAATA
CGTATTATAAAATATATAGAACTTATCAACTTTCCAATCAACTTTCTTTATTTTACAGAAATGGTACAAGTCTTCAACAA
ATTGTCCGTATATATCGTAATGAGCAAGATAACGATTTTCTTAAATTTCTGGGTGATTATCTTTTTAAAGAAGCTAATAA
AGGGCTCCCGTTACCTGTTATATTAATGAATTTAAAATGTTTTCAAAATGATTTGATTAAATTCATAGAACAAGGAGAGA
AAAATGGGAAATTAGATATAGAATTAAAGTTATACAGTCAAATGCTATTACAGCAATTTGAAGAAAAAGTGTTAAAACAA
ACAAAATTTATACAACCTATCATCTTCTTTATCTTGGGAATTTTTATTGTATCTTTATACTTAGTCATTATGCTTCCTAT
GTTTGAACTTATGCAAACAATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(225-346)

Type II secretion system (T2SS), protein F (T2SSF)

(26-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

49.719

100

0.499
  comGB Staphylococcus aureus N315

49.719

100

0.499