Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IUJ54_RS06970 Genome accession   NZ_CP064378
Coordinates   1595025..1595624 (-) Length   199 a.a.
NCBI ID   WP_014841277.1    Uniprot ID   A0AAV2UVV9
Organism   Legionella pneumophila subsp. pneumophila strain PartS-Lpneumophila-RM8376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1590025..1600624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ54_RS06950 (IUJ54_06945) aroB 1590272..1591381 (-) 1110 WP_010946668.1 3-dehydroquinate synthase -
  IUJ54_RS06955 (IUJ54_06950) aroK 1591368..1591889 (-) 522 WP_027219629.1 shikimate kinase AroK -
  IUJ54_RS06960 (IUJ54_06955) pilQ 1592341..1594437 (-) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  IUJ54_RS06965 (IUJ54_06960) pilP 1594441..1595028 (-) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  IUJ54_RS06970 (IUJ54_06965) pilO 1595025..1595624 (-) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  IUJ54_RS06975 (IUJ54_06970) pilN 1595638..1596186 (-) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  IUJ54_RS06980 (IUJ54_06975) pilM 1596190..1597254 (-) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  IUJ54_RS06985 (IUJ54_06980) ravI 1597671..1598681 (+) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22496.21 Da        Isoelectric Point: 5.3369

>NTDB_id=501399 IUJ54_RS06970 WP_014841277.1 1595025..1595624(-) (pilO) [Legionella pneumophila subsp. pneumophila strain PartS-Lpneumophila-RM8376]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=501399 IUJ54_RS06970 WP_014841277.1 1595025..1595624(-) (pilO) [Legionella pneumophila subsp. pneumophila strain PartS-Lpneumophila-RM8376]
ATGGACACGGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGTATCAGTATACTGATAATTGGATTAGGGTATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCAGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATTGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACTTTGCATGAATTTAGTGTTGAGGGGGTGTCATCCGGAGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGCTATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

99.497

100

0.995
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

37.696

95.98

0.362