Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IUJ54_RS06960 Genome accession   NZ_CP064378
Coordinates   1592341..1594437 (-) Length   698 a.a.
NCBI ID   WP_010946666.1    Uniprot ID   Q5ZX02
Organism   Legionella pneumophila subsp. pneumophila strain PartS-Lpneumophila-RM8376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1587341..1599437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ54_RS06940 (IUJ54_06935) - 1588284..1588715 (-) 432 WP_010946670.1 universal stress protein -
  IUJ54_RS06945 (IUJ54_06940) - 1588824..1590275 (-) 1452 WP_010946669.1 SPOR domain-containing protein -
  IUJ54_RS06950 (IUJ54_06945) aroB 1590272..1591381 (-) 1110 WP_010946668.1 3-dehydroquinate synthase -
  IUJ54_RS06955 (IUJ54_06950) aroK 1591368..1591889 (-) 522 WP_027219629.1 shikimate kinase AroK -
  IUJ54_RS06960 (IUJ54_06955) pilQ 1592341..1594437 (-) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  IUJ54_RS06965 (IUJ54_06960) pilP 1594441..1595028 (-) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  IUJ54_RS06970 (IUJ54_06965) pilO 1595025..1595624 (-) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  IUJ54_RS06975 (IUJ54_06970) pilN 1595638..1596186 (-) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  IUJ54_RS06980 (IUJ54_06975) pilM 1596190..1597254 (-) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  IUJ54_RS06985 (IUJ54_06980) ravI 1597671..1598681 (+) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77487.79 Da        Isoelectric Point: 9.5345

>NTDB_id=501397 IUJ54_RS06960 WP_010946666.1 1592341..1594437(-) (pilQ) [Legionella pneumophila subsp. pneumophila strain PartS-Lpneumophila-RM8376]
MRKIIAILILISASLSMAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSIYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=501397 IUJ54_RS06960 WP_010946666.1 1592341..1594437(-) (pilQ) [Legionella pneumophila subsp. pneumophila strain PartS-Lpneumophila-RM8376]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCCGAAAACAGAGTGCGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCTGCAA
GCTTTATTACTCAAAAGCCGCCCCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATATTGGAACTGGA
TCGTTCAGTATCCTATTCTGGCAGCACGGCGGGTTCCATTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCACGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGTGGTAGAGTCTTAATTGACGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAGAAGGTTCGCATGACCATTCTCAGCAAAGGGGATTATGGACATTTTGTGTAT
CAGGTCAATAAACAATTTATGGTTGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTAAAGAAACAGGT
ATTTACCGGTAAACGTATTTCTTTGAATTTCCAGAATATTAGTATTCGGGCTGTACTTCAATTGCTGGCAGATTTTACTG
GAATCAATATGGTTGTCAGCGACAAAGTACAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTG
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAAGGCAACGTCATGTTGATAGATACCAAGGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGTTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACCCACTTAAGTGGTACACTGGAAGGTGCTAACGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCAGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATACCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCGGTATTGAGTTTGAAAGTTACCCCACAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAGGAATTACTGATTTTTATTACTCCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAAA
AGGATGTTTATAAATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)

Bacterial type II and III secretion system protein (Secretin)

(525-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZX02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

100

100

1
  pilQ Legionella pneumophila strain ERS1305867

99.57

100

0.996
  pilQ Pseudomonas aeruginosa PAK

37.73

100

0.381
  comQ Acinetobacter baylyi ADP1

38.428

98.424

0.378
  pilQ Acinetobacter baumannii strain A118

35.095

100

0.371
  pilQ Acinetobacter baumannii D1279779

35.095

100

0.371