Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IUJ48_RS27285 Genome accession   NZ_CP064377
Coordinates   5933772..5935217 (-) Length   481 a.a.
NCBI ID   WP_024067399.1    Uniprot ID   A0AAX1D9P1
Organism   Achromobacter xylosoxidans strain PartM-Axylosoxidans-RM8376     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5928772..5940217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ48_RS27250 (IUJ48_27185) - 5929056..5929742 (+) 687 WP_006386994.1 hypothetical protein -
  IUJ48_RS27255 (IUJ48_27190) bfr 5929827..5930303 (-) 477 WP_006386993.1 bacterioferritin -
  IUJ48_RS27260 (IUJ48_27195) - 5930728..5931591 (-) 864 WP_227740234.1 DMT family transporter -
  IUJ48_RS27265 (IUJ48_27200) - 5931756..5932676 (+) 921 WP_006386991.1 LysR substrate-binding domain-containing protein -
  IUJ48_RS27270 (IUJ48_27205) - 5932646..5932945 (-) 300 WP_006386990.1 (2Fe-2S)-binding protein -
  IUJ48_RS27275 (IUJ48_27210) - 5933124..5933402 (-) 279 WP_006386989.1 type II toxin-antitoxin system RelE/ParE family toxin -
  IUJ48_RS27280 (IUJ48_27215) - 5933472..5933678 (-) 207 WP_024067400.1 hypothetical protein -
  IUJ48_RS27285 (IUJ48_27220) rpoN/rpoN1 5933772..5935217 (-) 1446 WP_024067399.1 RNA polymerase factor sigma-54 Machinery gene
  IUJ48_RS27290 (IUJ48_27225) - 5935216..5935866 (+) 651 WP_024067398.1 hypothetical protein -
  IUJ48_RS27295 (IUJ48_27230) - 5936059..5937036 (+) 978 WP_024067397.1 tripartite tricarboxylate transporter substrate binding protein -
  IUJ48_RS27300 (IUJ48_27235) leuA 5937427..5939136 (+) 1710 WP_006386984.1 2-isopropylmalate synthase -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52482.88 Da        Isoelectric Point: 4.8651

>NTDB_id=501376 IUJ48_RS27285 WP_024067399.1 5933772..5935217(-) (rpoN/rpoN1) [Achromobacter xylosoxidans strain PartM-Axylosoxidans-RM8376]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLELEAEISQVLADNPLLERDDEPASVETQAESERESSVQDDEP
APERDTPVEEMPGSGGVYPDDDSSLPEAARPDTLREHLLGQLVLTRAAPRDAALAALLIDELDENGYLGSPLEEILGWLP
AEMEPDLDELRAALSLLQSFDPAGIGARDMSECLLLQLRSPDTARLPEAADPKALACARLVCAQHLALLATGNHARLCAA
VGCDEATLRAAHALILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLANQKESAHAGL
QSQLQQAKWMIRNVEQRFDTILRVAQAIVDRQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGAGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMEKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=501376 IUJ48_RS27285 WP_024067399.1 5933772..5935217(-) (rpoN/rpoN1) [Achromobacter xylosoxidans strain PartM-Axylosoxidans-RM8376]
ATGCAAAATATTGTACCTATGACCCGTCCTATTTTAGAACTGCGGCCCGGCCAGCATCTGACGCTGACCCCTCAACTGCA
GCAATCGATCCGGTTGCTGCAGCTGTCCACGCTCGAGCTCGAGGCGGAAATCTCGCAAGTGCTCGCCGACAATCCGTTGC
TGGAACGCGACGATGAGCCTGCCTCCGTCGAGACGCAGGCCGAATCCGAGCGCGAGTCGTCGGTGCAGGACGACGAACCC
GCGCCCGAGCGCGACACCCCGGTCGAGGAAATGCCGGGTTCGGGCGGGGTCTACCCCGATGATGATTCCAGCCTGCCCGA
GGCCGCCCGGCCCGATACCCTGCGCGAGCACCTGCTCGGGCAACTGGTGCTGACCCGCGCCGCGCCGCGCGACGCCGCGC
TGGCGGCGCTGCTGATCGACGAACTGGACGAGAACGGCTACCTGGGCTCGCCCCTGGAGGAAATCCTGGGCTGGCTGCCG
GCCGAGATGGAGCCGGACCTGGACGAGCTGCGCGCCGCGCTGTCGCTGCTGCAATCGTTCGACCCGGCCGGCATCGGCGC
GCGCGACATGTCCGAGTGCCTGCTGTTGCAATTGCGCAGTCCCGACACGGCCCGGTTGCCCGAGGCCGCGGATCCCAAGG
CGCTGGCCTGCGCCCGCCTGGTCTGCGCCCAGCATCTGGCGCTGTTGGCCACCGGCAACCATGCCAGGCTGTGCGCCGCG
GTCGGTTGTGACGAGGCCACGCTGCGCGCGGCGCACGCCCTGATCCTGCGTCTGGAGCCCCGTCCGGGCCGCGCCTGGAC
GGTCCCGGCGGCCGATTACGCCGTGCCCGACGTCATCGTGCGCAAGACGCGCCGCGGCTGGCAGGTGACGCTCAACAGCG
CCGCCGTGCCGCGACTGCAGATCAATGGCCTGTACGCCCAAATGCTGGCCAACCAGAAGGAGTCGGCCCACGCCGGCTTG
CAGTCTCAGCTGCAACAGGCGAAATGGATGATCCGCAACGTGGAGCAGCGCTTCGACACCATCCTGCGAGTGGCCCAGGC
CATTGTCGACCGCCAGACCGCCTTCTTCAGCCAGGGGCCGGCGGCCATGCGCCCGCTCATCCTCAAGGACATTGCCGGCG
AGCTGGGCCTGCATGAGTCCACCATTTCACGCGCCACGACACAGAAGTACATGCTCACTCCCTTCGGTACGCTGGAGCTC
AAGCGCTTCTTCGGGGCGGGGGTGTCGACCGATGGCGGCGACGCCACATCCGCCACGGCGGTGCAGACTTTCATCCGCCA
GATGGTGGCCGAGGAGAACCGGGCCAAGCCGCTGTCCGACAGCCAGATCATGGAAAAACTGGCCGATCAGGGCATCGTCA
TCGCCCGCAGGACGGTCGCGAAATACCGCGAGGCACTGCGGATCGCGCCGGCCGCGCTGCGCAAGGCGCAGGCCGCGGCG
CGGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.505

100

0.52