Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   IRB21_RS19435 Genome accession   NZ_CP064089
Coordinates   3714699..3715826 (-) Length   375 a.a.
NCBI ID   WP_242731121.1    Uniprot ID   -
Organism   Bacillus altitudinis strain N435-3     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3709699..3720826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRB21_RS19400 flgL 3709882..3710793 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  IRB21_RS19405 flgK 3710802..3712322 (-) 1521 WP_024719239.1 flagellar hook-associated protein FlgK -
  IRB21_RS19410 - 3712338..3712820 (-) 483 WP_007499977.1 flagellar protein FlgN -
  IRB21_RS19415 flgM 3712839..3713102 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  IRB21_RS19420 - 3713182..3713601 (-) 420 WP_025208062.1 TIGR03826 family flagellar region protein -
  IRB21_RS19425 comFC 3713658..3714344 (-) 687 WP_172793290.1 ComF family protein Machinery gene
  IRB21_RS19430 - 3714341..3714634 (-) 294 WP_008348267.1 competence protein ComFB -
  IRB21_RS19435 comFA 3714699..3715826 (-) 1128 WP_242731121.1 DEAD/DEAH box helicase Machinery gene
  IRB21_RS19440 - 3716198..3717040 (-) 843 WP_024719242.1 DegV family protein -
  IRB21_RS19445 degU 3717252..3717941 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  IRB21_RS19450 degS 3718006..3719181 (-) 1176 WP_008360205.1 sensor histidine kinase Regulator
  IRB21_RS19455 - 3719403..3720071 (+) 669 WP_035701587.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42906.89 Da        Isoelectric Point: 10.3925

>NTDB_id=498988 IRB21_RS19435 WP_242731121.1 3714699..3715826(-) (comFA) [Bacillus altitudinis strain N435-3]
MLGKVSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKIGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQGQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
TESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEEEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=498988 IRB21_RS19435 WP_242731121.1 3714699..3715826(-) (comFA) [Bacillus altitudinis strain N435-3]
ATGCTTGGAAAAGTAAGTGAATGTGGCTTTCTCTATGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAACTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCATTTCCTTATTCAATGGATGAAAGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAATAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGGTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACTATTCAAGATGTTCAAGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
ACAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAGAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

56.25

98.133

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae D39

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae R6

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae TIGR4

40.96

94.4

0.387
  comFA/cflA Streptococcus mitis NCTC 12261

40.678

94.4

0.384
  comFA Latilactobacillus sakei subsp. sakei 23K

38.587

98.133

0.379
  comFA/cflA Streptococcus mitis SK321

39.548

94.4

0.373
  comFA Lactococcus lactis subsp. cremoris KW2

37.705

97.6

0.368