Detailed information NTG0498602

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: H9K44_RS03140
• Genome accession: NZ_CP064035
• Coordinates: 645694..647379 (-)
• Length: 561 a.a.
• NCBI ID: WP_169608822.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain DLM1799
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	639553..657115	645694..647379	within	0

Gene organization within MGE regions

Location: 639553..657115

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9K44_RS03095 (H9K44_03100)	rpsP	639553..639801 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
H9K44_RS03100 (H9K44_03105)	rimM	639830..640378 (+)	549	WP_005462552.1	ribosome maturation factor RimM	-
H9K44_RS03105 (H9K44_03110)	trmD	640406..641149 (+)	744	WP_005462562.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
H9K44_RS03110 (H9K44_03115)	rplS	641191..641544 (+)	354	WP_005462554.1	50S ribosomal protein L19	-
H9K44_RS03115 (H9K44_03120)	yacG	641867..642061 (-)	195	WP_005462546.1	DNA gyrase inhibitor YacG	-
H9K44_RS03120 (H9K44_03125)	zapD	642130..642870 (-)	741	WP_023624252.1	cell division protein ZapD	-
H9K44_RS03125 (H9K44_03130)	coaE	642898..643512 (-)	615	WP_005480887.1	dephospho-CoA kinase	-
H9K44_RS03130 (H9K44_03135)	pilD	643513..644382 (-)	870	WP_169608825.1	A24 family peptidase	Machinery gene
H9K44_RS03135 (H9K44_03140)	pilC	644447..645670 (-)	1224	WP_005479682.1	type II secretion system F family protein	Machinery gene
H9K44_RS03140 (H9K44_03145)	pilB	645694..647379 (-)	1686	WP_169608822.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H9K44_RS03145 (H9K44_03150)	-	647376..647834 (-)	459	WP_169608820.1	pilin	-
H9K44_RS03150 (H9K44_03155)	nadC	648097..648984 (-)	888	WP_015297249.1	carboxylating nicotinate-nucleotide diphosphorylase	-
H9K44_RS03155 (H9K44_03160)	ampD	649077..649628 (+)	552	WP_020841256.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
H9K44_RS03160 (H9K44_03165)	pdhR	650034..650801 (+)	768	WP_005462576.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
H9K44_RS03165 (H9K44_03170)	aceE	650850..653513 (+)	2664	WP_005462566.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
H9K44_RS03170 (H9K44_03175)	aceF	653533..655419 (+)	1887	WP_031830968.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
H9K44_RS03175 (H9K44_03180)	lpdA	655688..657115 (+)	1428	WP_005479684.1	dihydrolipoyl dehydrogenase	-

Sequence

Protein

• Length: 561 a.a.
• Molecular weight: 62374.21 Da
• Isoelectric Point: 5.6485

>NTDB_id=498602 H9K44_RS03140 WP_169608822.1 645694..647379(-) (pilB) [Vibrio parahaemolyticus strain DLM1799]
MITNLSTILRQAGLLTFSQEESLLEQVKASGISMPEALLSSGLFTSSELTEHLSSIFGLSQPEFSQYEYASLCQQLGLRE
LITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPNHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPDINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGIESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLGIQTTDN
IFRAHPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide

• Length: 1686 bp

>NTDB_id=498602 H9K44_RS03140 WP_169608822.1 645694..647379(-) (pilB) [Vibrio parahaemolyticus strain DLM1799]
ATGATAACCAACCTCTCAACAATTCTTCGCCAAGCAGGATTACTAACATTTTCCCAAGAAGAATCGTTGCTTGAGCAAGT
CAAGGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACATCAAGCGAACTGACCGAGCACTTAA
GTTCTATTTTTGGCTTAAGTCAGCCCGAGTTCTCTCAATATGAATATGCTTCGCTTTGCCAACAACTCGGCCTGCGTGAA
TTAATCACACGACATAATGCACTCCCGCTCCACCGTACTCCTTCAACTTTATTATTAGCGGTTGCCGACCCCACGAATCA
ACAAGCTGAAGATGATTTCCGCTTTGCCACTGGTTTACAGGTTGAATTGGTGTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATCCGACGATTATATGGTCGTTCATTGAGTCACGAGAAGTCTGGATTAAAAGAGATCAACCAAGAAGAATTAGCCAAC
CTGGTTGATGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGAGTTCGTC
TACGTTGCGATGGCATTTTGATCGAAACTCAGCAACCGCCAAATCACTTGAGTCGTCGCTTATCAGCTCGTATAAAAATT
CTCTCTAAACTTGATATTGCCGAGCGACGTTTACCACAAGATGGGCGAATTAAACTCAAACTGAATCAAGACACCGCGAT
TGATATGCGGGTATCCACTCTACCTACCTTGTTTGGAGAGAAGATCGTACTACGACTGCTCGATAGCAGCTCTGCATCAC
TGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAGCTGTATTTAGAGGCTCTGCGTCGTCCACAAGGTATGATT
CTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGGCTAAATATTCTCAATAAACCAGATATCAA
CATCTCCACAGCGGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTTTTG
GCTTTGCAGAAGCACTACGTTCTTTTCTGCGTCAAGATCCCGATGTGGTCATGGTGGGTGAAATTCGAGACCTAGATACT
GCGGAAATCGCCATCAAGGCTTCCCAAACCGGTCACTTAGTGCTCTCTACTTTGCATACCAACTCTGCGGCTGAAACCAT
CATTCGTTTATCAAACATGGGCATAGAAAGTTTTAACCTCGCCTCTTCTCTTAGTTTGATTATTGCCCAACGCCTAGCTC
GCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGGCATTCAAACAACTGACAAC
ATTTTTAGAGCCCATCCAGATGGTTGCAACGAATGCACACATGGTTATTCTGGCCGAACAGGTATCTATGAAGTCATGCG
CTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCGGTACATGAACTGGAAAAGCTTGCCATTGCGAACGGCA
TGAGCACCTTGCAAATGTCCGGAATAGAAAAACTCAAACAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTCTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (38-144)

• Type II/IV secretion system protein (T2SSE) (188-455)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio parahaemolyticus RIMD 2210633	97.683	100	0.977
pilB	Vibrio campbellii strain DS40M4	90.196	100	0.902
pilB	Vibrio cholerae strain A1552	74.021	100	0.742
pilB	Acinetobacter baumannii D1279779	51.711	93.761	0.485
pilB	Legionella pneumophila strain ERS1305867	48.201	99.109	0.478
pilB	Acinetobacter baylyi ADP1	50.382	93.405	0.471
pilF	Neisseria gonorrhoeae MS11	45.035	100	0.453
pilB/pilB1	Synechocystis sp. PCC 6803	36.975	100	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	36.667	100	0.373
pilF	Thermus thermophilus HB27	36.525	100	0.367