Detailed information NTG0496671

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IOD65_RS15215
• Genome accession: NZ_CP063769
• Coordinates: 3186986..3188104 (+)
• Length: 372 a.a.
• NCBI ID: WP_005070855.1
• Uniprot ID: A0A013SVN8
• Organism: Acinetobacter sp. Ac-14
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3181986..3193104

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IOD65_RS15205 (IOD65_15205)	-	3185110..3185802 (-)	693	WP_005070852.1	YggS family pyridoxal phosphate-dependent enzyme	-
IOD65_RS15210 (IOD65_15210)	pilT	3185929..3186966 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
IOD65_RS15215 (IOD65_15215)	pilU	3186986..3188104 (+)	1119	WP_005070855.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IOD65_RS15220 (IOD65_15220)	fur	3188174..3188611 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
IOD65_RS15225 (IOD65_15225)	-	3188723..3189121 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
IOD65_RS15230 (IOD65_15230)	-	3189186..3189500 (-)	315	WP_005070859.1	RnfH family protein	-
IOD65_RS15235 (IOD65_15235)	-	3189500..3190567 (-)	1068	WP_005070863.1	hypothetical protein	-
IOD65_RS15240 (IOD65_15240)	-	3190721..3191176 (-)	456	WP_002121698.1	bacteriohemerythrin	-
IOD65_RS15245 (IOD65_15245)	-	3191428..3192276 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41931.15 Da
• Isoelectric Point: 6.6404

>NTDB_id=496671 IOD65_RS15215 WP_005070855.1 3186986..3188104(+) (pilU) [Acinetobacter sp. Ac-14]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=496671 IOD65_RS15215 WP_005070855.1 3186986..3188104(+) (pilU) [Acinetobacter sp. Ac-14]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTAAGGCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTGCTTAAAGATTTATCAATGACCAAACGCGGTATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTTGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A013SVN8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363