Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INQ42_RS09865 Genome accession   NZ_CP063657
Coordinates   2181589..2183319 (+) Length   576 a.a.
NCBI ID   WP_194034115.1    Uniprot ID   -
Organism   Novilysobacter avium strain H23M41     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2168194..2181466 2181589..2183319 flank 123


Gene organization within MGE regions


Location: 2168194..2183319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ42_RS09820 (INQ42_09820) - 2168194..2168886 (-) 693 WP_194034106.1 M949_RS01915 family surface polysaccharide biosynthesis protein -
  INQ42_RS09825 (INQ42_09825) - 2169825..2172611 (-) 2787 WP_194034107.1 glycoside hydrolase family 19 protein -
  INQ42_RS09830 (INQ42_09830) - 2172644..2175241 (-) 2598 WP_194034108.1 type VI secretion system Vgr family protein -
  INQ42_RS09835 (INQ42_09835) comP 2175636..2176067 (+) 432 WP_194034109.1 pilin Machinery gene
  INQ42_RS09840 (INQ42_09840) comP 2176172..2176597 (+) 426 WP_194034110.1 pilin Machinery gene
  INQ42_RS09845 (INQ42_09845) - 2176676..2178745 (+) 2070 WP_194034111.1 sulfatase-like hydrolase/transferase -
  INQ42_RS09850 (INQ42_09850) - 2178859..2180430 (+) 1572 WP_194034112.1 hypothetical protein -
  INQ42_RS09855 (INQ42_09855) - 2180773..2181075 (+) 303 WP_194034113.1 type II toxin-antitoxin system HigB family toxin -
  INQ42_RS09860 (INQ42_09860) - 2181092..2181466 (+) 375 WP_194034114.1 type II toxin-antitoxin system HigA family antitoxin -
  INQ42_RS09865 (INQ42_09865) pilB 2181589..2183319 (+) 1731 WP_194034115.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 63002.23 Da        Isoelectric Point: 5.6042

>NTDB_id=496151 INQ42_RS09865 WP_194034115.1 2181589..2183319(+) (pilB) [Novilysobacter avium strain H23M41]
MNAVATANLVGITGIARRLVQDGALSESAAREAMAAATEQRKPLAAYIIEKRLVAPAHLAAANSVEFGVPIFDAAAMDPE
QSAIKLVKEELLRKHTVLPLFKRGNRLFVGISEPTNTHALSEIKFQTNFTVEAILVDEESIKRHLDRWQENADALGDAMG
EDDEGLENLDIGGGEDELSSESGVDAKTDDTPVVKFINKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQSAKVPVK
LQPRISARLKVMAQLDIAERRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKMGIEKLGYEDDQRDLF
LAAVKRPYGMVLVTGPTGSGKTVSLYTALNILNDETRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVSLVIAQRLARRLHDCKRPVELPEHA
LLAEGFTAEEIASEDFTIYEAVGCADCTEGYKGRTGIYQVMPMTDEIQGIVLAGGNAMQIADAAQKSGVRDLRQSALMKV
RNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=496151 INQ42_RS09865 WP_194034115.1 2181589..2183319(+) (pilB) [Novilysobacter avium strain H23M41]
ATGAATGCCGTAGCTACTGCCAACCTTGTTGGCATCACCGGTATTGCCCGCCGCCTGGTGCAGGATGGCGCGCTGTCCGA
GTCTGCTGCACGTGAGGCCATGGCCGCCGCCACCGAGCAACGCAAGCCGCTCGCCGCGTACATCATCGAGAAGCGACTCG
TCGCTCCCGCGCATCTTGCCGCGGCCAACTCTGTCGAGTTCGGTGTACCGATTTTCGATGCCGCCGCGATGGACCCGGAG
CAGTCGGCGATCAAGCTGGTCAAGGAAGAGCTGCTTCGCAAGCACACGGTGCTGCCGCTGTTCAAGCGCGGCAACCGCCT
GTTCGTCGGTATTTCCGAACCCACCAACACGCACGCGTTGTCGGAGATCAAGTTCCAGACCAACTTCACCGTCGAGGCGA
TCCTGGTCGACGAGGAAAGCATCAAGCGCCATCTGGACCGCTGGCAGGAGAACGCCGATGCACTCGGCGACGCCATGGGC
GAGGACGACGAGGGGCTTGAGAACCTGGACATCGGCGGCGGGGAGGACGAACTCTCATCCGAGAGCGGGGTCGACGCCAA
AACGGACGACACCCCTGTCGTCAAGTTCATCAACAAGGTGCTGGTGGATGCGATCCGTCGCGGCGCGTCTGACATCCACT
TCGAGCCGTATGAAACCGAGTACCGGGTGCGCCTGCGCATCGATGGGCTCCTGAAGCAGTCGGCCAAGGTGCCGGTCAAA
TTGCAGCCGCGCATATCCGCACGCCTCAAGGTGATGGCGCAGCTGGATATCGCCGAGCGCCGTGTACCGCAGGACGGGCG
CATCAAGCTCAACCTGAGCAAGACCAAGCAGATCGACTTTCGAGTCAGCACGCTGCCGACTCTATTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCGGCGAAGATGGGCATTGAGAAGCTCGGCTACGAGGACGACCAGCGCGACCTGTTC
CTGGCCGCGGTCAAGCGTCCCTACGGGATGGTGCTGGTCACCGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTGTACAC
GGCGCTGAACATTCTCAACGACGAGACGCGCAACATCTCCACCGTTGAGGATCCGGTCGAGATCCGCGTGCCGGGCATCA
ATCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGTGAGATCCGCGACCTGGAAACCGCCGAGATCGGCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCACCTCGT
CGGTGTCGCTGGTGATCGCCCAGCGTCTGGCCCGCCGCCTGCACGACTGCAAGCGCCCGGTGGAACTGCCCGAGCACGCG
CTGCTGGCCGAAGGCTTTACCGCCGAGGAGATCGCGTCTGAAGACTTCACGATCTACGAGGCGGTTGGGTGCGCCGACTG
CACCGAAGGCTACAAGGGCCGTACCGGCATCTACCAGGTCATGCCGATGACCGACGAGATCCAGGGCATCGTGCTGGCGG
GGGGCAACGCGATGCAGATTGCCGATGCCGCGCAGAAGTCCGGCGTGCGCGACCTGCGCCAGTCGGCCTTGATGAAGGTC
CGCAATGGCGTCACAAGCCTGGCGGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.106

98.611

0.543
  pilB Acinetobacter baylyi ADP1

53.792

98.438

0.53
  pilB Legionella pneumophila strain ERS1305867

49.472

98.611

0.488
  pilB Vibrio cholerae strain A1552

52.611

89.757

0.472
  pilF Neisseria gonorrhoeae MS11

47.711

98.611

0.47
  pilB Vibrio campbellii strain DS40M4

50.81

85.764

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

50.403

86.111

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.353

100

0.387
  pilF Thermus thermophilus HB27

36.61

100

0.375