Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IM816_RS04690 Genome accession   NZ_CP063231
Coordinates   1083895..1085622 (-) Length   575 a.a.
NCBI ID   WP_250339971.1    Uniprot ID   -
Organism   Luteibacter flocculans strain EIF3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1078895..1090622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM816_RS04670 (IM816_04675) - 1079682..1080932 (+) 1251 WP_072322917.1 bifunctional diguanylate cyclase/phosphodiesterase -
  IM816_RS04675 (IM816_04680) coaE 1080945..1081550 (-) 606 WP_250339968.1 dephospho-CoA kinase -
  IM816_RS04680 (IM816_04685) - 1081547..1082416 (-) 870 WP_250339969.1 A24 family peptidase -
  IM816_RS04685 (IM816_04690) pilC 1082495..1083760 (-) 1266 WP_250339970.1 type II secretion system F family protein Machinery gene
  IM816_RS04690 (IM816_04695) pilB 1083895..1085622 (-) 1728 WP_250339971.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IM816_RS04695 (IM816_04700) - 1085805..1087202 (+) 1398 WP_250339972.1 MBOAT family O-acyltransferase -
  IM816_RS04700 (IM816_04705) - 1087171..1088346 (+) 1176 WP_250339973.1 hypothetical protein -
  IM816_RS04705 (IM816_04710) - 1088343..1089197 (+) 855 WP_250339974.1 glycosyltransferase family 2 protein -
  IM816_RS04710 (IM816_04715) - 1089274..1090392 (+) 1119 WP_250339975.1 glycosyltransferase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62670.39 Da        Isoelectric Point: 6.3654

>NTDB_id=493972 IM816_RS04690 WP_250339971.1 1083895..1085622(-) (pilB) [Luteibacter flocculans strain EIF3]
MASQMHQPMLAGLTGMARRLVSEGVLPEADVRKAVQDAAEKRVSLSAWLVDHNLVDSGKLSQVASSEFGMPLMDIGSMAP
GAMPLDLVTEALITKHQALPLFKRGKRLFVGIADPMQSHALDEIKFHSNHMVEPVLVERGQLRRVIDSALSAMNANVPGF
EDGGLDELVMETGDDDGEGTTGIDANANDDAPVVKFVNKILVDAIKRGASDIHFEPFETVYRVRLRMDGILRIVATAPIK
LGNRIASRIKVMSGLDIAERRVPQDGRIKLNLTKTRAIDFRVSTLPTLFGEKIVLRILDGSSAKLGIDKLGYEELQKKLY
LEAIEKPYGMVLVTGPTGSGKTVSLYTALNILNTEGRNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPYNITSSVTLIIAQRLARRLHDCKRAIQLPPAA
LLAEGFTQEEIDEGITIYEAVGCDSCNEGYKGRVGIYQVMPMVEDIQKIVLEGGNALQIAEVARKAGINDLRASALLKVR
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=493972 IM816_RS04690 WP_250339971.1 1083895..1085622(-) (pilB) [Luteibacter flocculans strain EIF3]
ATGGCATCACAAATGCATCAGCCCATGCTCGCCGGCTTGACCGGCATGGCACGGCGCCTCGTCAGTGAAGGTGTGCTGCC
AGAGGCGGACGTGCGCAAGGCCGTGCAGGATGCCGCCGAGAAACGTGTTTCTCTCTCAGCCTGGCTGGTTGACCACAATC
TGGTCGACAGCGGCAAGCTGTCTCAGGTGGCCTCGTCGGAATTCGGCATGCCGTTGATGGACATCGGTTCCATGGCGCCG
GGCGCGATGCCACTGGACCTGGTGACCGAGGCTTTGATCACGAAGCACCAGGCACTTCCCCTGTTCAAGCGCGGCAAGCG
GCTGTTCGTCGGCATCGCCGACCCGATGCAGTCGCACGCGCTCGACGAGATCAAGTTCCACTCGAACCACATGGTCGAGC
CGGTACTGGTCGAGCGTGGGCAGTTGCGCCGGGTGATCGATAGCGCCCTTTCGGCGATGAACGCCAACGTGCCCGGTTTC
GAGGACGGCGGGCTCGACGAACTGGTAATGGAAACGGGCGACGACGACGGCGAAGGCACCACCGGCATCGACGCGAACGC
CAACGACGACGCCCCGGTCGTCAAGTTCGTGAACAAGATCCTGGTGGACGCCATCAAGCGCGGCGCCTCGGATATCCACT
TCGAGCCGTTCGAAACGGTTTATCGCGTGCGCCTGCGAATGGACGGCATCCTGCGCATCGTAGCCACTGCGCCGATCAAG
CTGGGCAACCGGATCGCCTCGCGCATCAAGGTCATGAGCGGCCTGGACATCGCCGAACGCCGCGTGCCGCAAGATGGCCG
CATCAAGCTCAACCTGACCAAGACCCGCGCGATCGACTTCCGCGTCAGCACGTTGCCCACGCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGTTCGTCGGCCAAGCTCGGCATCGACAAGCTGGGTTACGAGGAACTCCAGAAGAAGCTGTAT
CTGGAAGCTATCGAGAAACCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGTAAGACTGTGTCGCTGTATAC
GGCGCTCAACATCCTCAACACCGAGGGACGCAATATCTCCACGGTGGAAGACCCGGTCGAAATCCGCGTCGAGGGCATCA
ATCAGGTTCAGCAGAACACCAAGCGCGGCATGACCTTCGCGGCCGCCCTACGCTCCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGAGATCGCCGTGAAAGCCGCCCAAACCGGCCATATGGTGCTGTC
CACCCTGCACACGAACGACGCGCCACAGACGATCTCCCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCTCCT
CGGTCACCCTGATCATCGCCCAGCGCCTGGCCCGACGCCTGCACGACTGCAAGCGGGCGATCCAGCTCCCGCCGGCCGCA
CTGCTGGCGGAGGGGTTCACCCAGGAGGAAATCGACGAGGGCATCACCATCTACGAAGCGGTGGGGTGCGACAGCTGCAA
CGAGGGCTACAAGGGCCGCGTGGGCATATACCAGGTCATGCCCATGGTCGAGGACATCCAGAAGATCGTCCTGGAGGGGG
GCAACGCTCTCCAGATCGCCGAAGTGGCCCGCAAGGCCGGGATCAACGACCTCCGCGCCTCGGCGCTGCTCAAGGTGAGG
AACGGTGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(20-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.124

98.435

0.543
  pilB Acinetobacter baylyi ADP1

54.69

98.261

0.537
  pilB Legionella pneumophila strain ERS1305867

50.436

99.652

0.503
  pilB Vibrio cholerae strain A1552

48.496

98.261

0.477
  pilF Neisseria gonorrhoeae MS11

50.094

92.348

0.463
  pilB Vibrio campbellii strain DS40M4

46.573

98.957

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

47.406

97.217

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.256

100

0.374
  pilF Thermus thermophilus HB27

37.343

96.87

0.362