Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INQ48_RS25980 Genome accession   NZ_CP063166
Coordinates   5552527..5554260 (+) Length   577 a.a.
NCBI ID   WP_203202656.1    Uniprot ID   -
Organism   Variovorax paradoxus strain VAI-C     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5547527..5559260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ48_RS25950 (INQ48_25930) proB 5548156..5549295 (-) 1140 WP_203202653.1 glutamate 5-kinase -
  INQ48_RS25955 (INQ48_25935) cgtA 5549374..5550450 (-) 1077 WP_203202654.1 Obg family GTPase CgtA -
  INQ48_RS25960 (INQ48_25940) rpmA 5550522..5550782 (-) 261 WP_057592096.1 50S ribosomal protein L27 -
  INQ48_RS25965 (INQ48_25945) rplU 5550795..5551106 (-) 312 WP_057592097.1 50S ribosomal protein L21 -
  INQ48_RS25970 (INQ48_25950) - 5551292..5552284 (+) 993 WP_203202655.1 polyprenyl synthetase family protein -
  INQ48_RS25980 (INQ48_25960) pilB 5552527..5554260 (+) 1734 WP_203202656.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INQ48_RS25985 (INQ48_25965) pilC 5554291..5555526 (+) 1236 WP_203202657.1 type II secretion system F family protein Machinery gene
  INQ48_RS25990 (INQ48_25970) - 5555526..5556491 (+) 966 WP_203202658.1 A24 family peptidase -
  INQ48_RS25995 (INQ48_25975) coaE 5556508..5557116 (+) 609 WP_203202659.1 dephospho-CoA kinase -
  INQ48_RS26000 (INQ48_25980) - 5557191..5558681 (+) 1491 WP_203202660.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62648.90 Da        Isoelectric Point: 6.0490

>NTDB_id=493684 INQ48_RS25980 WP_203202656.1 5552527..5554260(+) (pilB) [Variovorax paradoxus strain VAI-C]
MAAAELPVKENPQVALPGLARALVSAGKLPVKTAEDLYQKSLSSRSSFIAELTGTGAVSASDLAHTLSNAFGAPLLDLDA
IDHQRLPKDLLDPKLCQAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMVEAAAASAAETI
NSIVGTEFEFDDVSADTAGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAISRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMTCRTPADIPR
EALIEAGFKEHEVDGSWKPYRPVGCAACTGGYKGRVGIYQVMPISEAIQEIILRDGSALDIAQQSEAEGVRSLRKSGLRK
VMQGLTSLEEVVAVTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=493684 INQ48_RS25980 WP_203202656.1 5552527..5554260(+) (pilB) [Variovorax paradoxus strain VAI-C]
ATGGCTGCAGCCGAACTTCCCGTTAAAGAAAACCCGCAAGTCGCCTTGCCAGGGCTGGCACGCGCGCTGGTTTCGGCGGG
CAAGCTGCCGGTGAAGACGGCGGAGGATCTTTATCAGAAGTCGTTGAGCAGCCGCTCGAGCTTCATCGCCGAGCTCACGG
GCACCGGCGCCGTCTCGGCCTCCGACCTCGCTCACACGCTGTCGAATGCGTTCGGCGCCCCGCTGCTCGACCTCGACGCC
ATCGACCACCAGCGCCTGCCGAAGGACCTGCTAGATCCCAAGCTCTGCCAGGCCTATCGCATCGTCGTCCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCCACGGCCGATCCGTCCGACCAGCAGGCCGCCGAGAAGATCAAGTTCGCCTCCCAGATGG
GCGTGGACTGGGTCATCGCCGAGTACGACAAGCTCTCGCGCATGGTCGAGGCCGCCGCCGCGAGCGCGGCCGAAACCATC
AACAGCATCGTCGGCACGGAGTTCGAGTTCGACGACGTCAGCGCCGACACCGCGGGCGACGCCAATGAGCAGGCGATCGC
CGAGGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGCGGTCAGCATGCGCGCCTCGGACATCC
ACTTCGAGCCCTACGAGCACCACTACCGCGTGCGCTTCCGGGTCGACGGCGAACTGCGCGAGGTCGCGAGCCCGCCCACG
GTGATCAAGGACAAGCTGGCGTCGCGCATCAAGGTCATCTCGCGGCTCGACATCTCCGAGAAGCGCGTGCCCCAGGACGG
GCGCATGAAGCTCAAGATCGGTCCCGACCGGGTGATCGACTTCCGCGTGAGCACACTGCCCACGCTGTTCGGCGAGAAGA
TCGTGGTCCGGATTCTCGACCCCAGCAGCGCGCGCATGGGCATCGATGCGCTGGGCTACGACGCCGACGAGAAGGAACGC
CTGCTGCACGCCATCAGCCGCCCCTATGGCATGGTGCTCGTGACCGGACCGACCGGTTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTCAACCTGCTGAACCAGCCGGGCGTGAACATCGCGACCGCCGAGGACCCGTCGGAAATCAACCTGCCGGGCG
TCAACCAGGTCAATGTGAACGAGAAGGCCGGCCTGACCTTCGCGGCCGCGCTGCGGGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGCCACCTGGTGCT
GTCGACGCTGCACACCAACGACGCCCCCACCACCCTCACGCGGATGCGCAACATGGGCATCGCGCCCTTCAACATCGCAT
CGAGCGTGATCCTGATCACCGCCCAGCGGCTCGCGCGGCGGCTGTGCATGACCTGCCGCACGCCCGCGGACATCCCGCGC
GAGGCGCTGATCGAGGCCGGCTTCAAGGAGCACGAGGTCGACGGCAGCTGGAAGCCCTACCGGCCCGTGGGCTGCGCGGC
CTGCACCGGCGGCTACAAGGGCCGGGTGGGCATCTACCAGGTGATGCCGATCTCCGAGGCGATCCAGGAGATCATCCTGC
GCGACGGCAGCGCGCTCGACATCGCGCAGCAGTCGGAAGCCGAAGGCGTGCGCTCGCTGCGCAAGTCGGGCCTGCGCAAA
GTGATGCAGGGCCTCACCTCGCTCGAGGAAGTGGTGGCGGTCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-156)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilB Acinetobacter baylyi ADP1

53.086

98.267

0.522
  pilF Neisseria gonorrhoeae MS11

52.847

97.4

0.515
  pilB Legionella pneumophila strain ERS1305867

49.113

97.747

0.48
  pilB Vibrio campbellii strain DS40M4

45.745

97.747

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

97.4

0.447
  pilB Vibrio cholerae strain A1552

47.5

90.121

0.428
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.643

91.681

0.373
  pilF Thermus thermophilus HB27

37.566

98.267

0.369