Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IL982_RS00830 Genome accession   NZ_CP063049
Coordinates   136478..136912 (-) Length   144 a.a.
NCBI ID   WP_197573560.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain KC-Dl-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 131478..141912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL982_RS00810 (IL982_00810) coaE 131828..132430 (-) 603 WP_086367532.1 dephospho-CoA kinase -
  IL982_RS00815 (IL982_00815) pilD 132432..133310 (-) 879 WP_188013997.1 prepilin peptidase Machinery gene
  IL982_RS00820 (IL982_00820) pilC 133493..134719 (-) 1227 WP_086367530.1 type II secretion system F family protein Machinery gene
  IL982_RS00825 (IL982_00825) pilB 134786..136477 (-) 1692 WP_197573559.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IL982_RS00830 (IL982_00830) pilA 136478..136912 (-) 435 WP_197573560.1 pilin Machinery gene
  IL982_RS00835 (IL982_00835) ampD 137333..137911 (+) 579 WP_086367527.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IL982_RS00840 (IL982_00840) pdhR 138271..139044 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IL982_RS00845 (IL982_00845) aceE 139125..141785 (+) 2661 WP_188013993.1 pyruvate dehydrogenase (acetyl-transferring), homodimeric type -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15023.22 Da        Isoelectric Point: 4.7871

>NTDB_id=492547 IL982_RS00830 WP_197573560.1 136478..136912(-) (pilA) [Photobacterium damselae subsp. damselae strain KC-Dl-1]
MKGQKGFTLIELMIVVAIIGVLSAIAIPAYKDYVSKSEYASAIATVRALVTEAELIYQEKGSLAADTPLTDLGTAADGKA
NPLGTISIASANNIKFLFGSDSSLNTAFFTFSRADDGWKCVGTNVGSVKLPKIDACAAPAQQTE

Nucleotide


Download         Length: 435 bp        

>NTDB_id=492547 IL982_RS00830 WP_197573560.1 136478..136912(-) (pilA) [Photobacterium damselae subsp. damselae strain KC-Dl-1]
ATGAAAGGACAAAAGGGTTTTACCTTAATTGAATTGATGATCGTAGTGGCGATCATAGGTGTGTTATCAGCAATCGCAAT
TCCAGCTTACAAAGATTACGTATCAAAAAGTGAATATGCGTCAGCTATAGCAACAGTTAGAGCTTTAGTAACGGAAGCTG
AACTGATTTATCAAGAGAAAGGTTCATTAGCGGCAGATACTCCACTAACAGATCTAGGTACAGCAGCTGATGGTAAAGCT
AATCCATTGGGAACAATATCTATAGCATCAGCAAATAATATAAAATTCTTATTTGGTTCAGATAGTTCGTTAAATACTGC
ATTTTTTACCTTTTCCCGTGCAGACGATGGTTGGAAATGTGTAGGAACAAATGTGGGGTCTGTCAAACTACCAAAAATAG
ATGCTTGTGCAGCGCCAGCTCAACAGACCGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

55.556

87.5

0.486
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.568

100

0.438
  pilA Vibrio cholerae strain A1552

42.568

100

0.438
  pilA Vibrio cholerae C6706

42.568

100

0.438
  pilA Pseudomonas aeruginosa PAK

45.652

95.833

0.437
  pilA Acinetobacter baumannii strain A118

37.162

100

0.382