Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   KIM43_RS30555 Genome accession   NZ_CP074773
Coordinates   5881368..5882888 (+) Length   506 a.a.
NCBI ID   WP_000058976.1    Uniprot ID   -
Organism   Escherichia coli strain 97_3250     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 5876368..5887888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM43_RS30530 (KIM43_30105) ilvE 5877922..5878851 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  KIM43_RS30535 (KIM43_30110) ilvM 5878871..5879134 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  KIM43_RS30540 (KIM43_30115) ilvG 5879131..5880777 (-) 1647 WP_001012624.1 acetolactate synthase 2 catalytic subunit -
  KIM43_RS30545 (KIM43_30120) ilvX 5880780..5880830 (-) 51 WP_001387183.1 peptide IlvX -
  KIM43_RS30550 (KIM43_30125) ilvL 5880917..5881015 (-) 99 WP_001375501.1 ilv operon leader peptide -
  KIM43_RS30555 (KIM43_30130) comM 5881368..5882888 (+) 1521 WP_000058976.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  KIM43_RS30560 (KIM43_30135) maoP 5882913..5883251 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  KIM43_RS30565 (KIM43_30140) hdfR 5883370..5884209 (+) 840 WP_000379246.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55132.33 Da        Isoelectric Point: 7.6191

>NTDB_id=492458 KIM43_RS30555 WP_000058976.1 5881368..5882888(+) (comM) [Escherichia coli strain 97_3250]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=492458 KIM43_RS30555 WP_000058976.1 5881368..5882888(+) (comM) [Escherichia coli strain 97_3250]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACTGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTGGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCGCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGTTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCCAAAATAACCTATCCAGCCCGTTTCCAGCTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterProScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.708

100

0.593
  comM Vibrio cholerae O1 biovar El Tor strain E7946

59.363

99.209

0.589
  comM Vibrio cholerae strain A1552

59.363

99.209

0.589
  comM Glaesserella parasuis strain SC1401

58.58

100

0.587
  comM Vibrio campbellii strain DS40M4

58.167

99.209

0.577
  comM Acinetobacter baylyi ADP1

48.996

98.419

0.482
  comM Legionella pneumophila str. Paris

47.992

98.419

0.472
  comM Legionella pneumophila strain ERS1305867

47.992

98.419

0.472
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443