NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	IL989_RS11605	Genome accession	NZ_CP062984
Coordinates	2385854..2386924 (-)	Length	356 a.a.
NCBI ID	WP_193350432.1	Uniprot ID	-
Organism	Bacillus amyloliquefaciens strain PP19 isolate PP19
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2380854..2391924

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IL989_RS11555 (IL989_11555)	sipW	2380913..2381497 (-)	585	WP_071348018.1	signal peptidase I SipW	-
IL989_RS11560 (IL989_11560)	tapA	2381469..2382140 (-)	672	WP_193350429.1	amyloid fiber anchoring/assembly protein TapA	-
IL989_RS11565 (IL989_11565)	-	2382398..2382727 (+)	330	WP_045510605.1	DUF3889 domain-containing protein	-
IL989_RS11570 (IL989_11570)	-	2382768..2382947 (-)	180	WP_016938971.1	YqzE family protein	-
IL989_RS11575 (IL989_11575)	comGG	2383004..2383381 (-)	378	WP_193350430.1	competence type IV pilus minor pilin ComGG	Machinery gene
IL989_RS11580 (IL989_11580)	comGF	2383383..2383883 (-)	501	WP_227014200.1	competence type IV pilus minor pilin ComGF	-
IL989_RS11585 (IL989_11585)	comGE	2383792..2384106 (-)	315	WP_193350431.1	competence type IV pilus minor pilin ComGE	Machinery gene
IL989_RS11590 (IL989_11590)	comGD	2384090..2384527 (-)	438	WP_065522768.1	competence type IV pilus minor pilin ComGD	Machinery gene
IL989_RS11595 (IL989_11595)	comGC	2384517..2384825 (-)	309	WP_081301877.1	competence type IV pilus major pilin ComGC	Machinery gene
IL989_RS11600 (IL989_11600)	comGB	2384830..2385867 (-)	1038	WP_013352871.1	competence type IV pilus assembly protein ComGB	Machinery gene
IL989_RS11605 (IL989_11605)	comGA	2385854..2386924 (-)	1071	WP_193350432.1	competence type IV pilus ATPase ComGA	Machinery gene
IL989_RS11610 (IL989_11610)	-	2387118..2388068 (-)	951	WP_013352873.1	magnesium transporter CorA family protein	-
IL989_RS11615 (IL989_11615)	-	2388215..2389516 (+)	1302	WP_193350433.1	hemolysin family protein	-
IL989_RS11620 (IL989_11620)	-	2389554..2390384 (-)	831	WP_193350434.1	STAS domain-containing protein	-
IL989_RS11630 (IL989_11630)	-	2390597..2390977 (-)	381	WP_013352876.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
IL989_RS11635 (IL989_11635)	-	2391212..2391457 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40528.82 Da Isoelectric Point: 9.0345

                                >NTDB_id=492259 IL989_RS11605 WP_193350432.1 2385854..2386924(-) (comGA) [Bacillus amyloliquefaciens strain PP19 isolate PP19]

MENIETYSRNIINEAYMARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAVLLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCETDNCSVYCRLYRQTRRAGIYELLYGKNLTQCFKEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=492259 IL989_RS11605 WP_193350432.1 2385854..2386924(-) (comGA) [Bacillus amyloliquefaciens strain PP19 isolate PP19]

ATGGAGAATATTGAAACATACAGCAGGAACATTATCAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTTCC

GAGGGAAAGGGATGCTTTCATTCATTTTCGCATCGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTTAAATTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCACTGTCT

CTGCCGCTTCCAACGGAAACCGTTCATTTGAGGATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

ATTGCCTAAGAGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGTATTATTATCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTTTTTACCGGCCCGACCGGATCTGGAAAAACAACTACCCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTGGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCACCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG

GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTACTGTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTCGGGGTGAACATGACGGAAATTGAACAAACTGTTATAGC

CATCGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCGAAACGGACAACTGTTCCGTATACTGCCGTCTGTACA

GGCAGACACGCCGTGCGGGGATTTATGAACTGCTTTACGGAAAAAATCTCACCCAGTGCTTTAAAGAAGCAAAAGGAGAG

CACGCCAATTTTCAATATCAGACGCTTCGCAGGCTGATCAGAAAAGGCATCGCGCTCGGTTATGTCACGACTGACAATTA

TGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.303	100	0.823
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.003	95.787	0.374

Detailed information NTG0492259