Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LP316_RS00790 Genome accession   NZ_CP062946
Coordinates   186093..187793 (+) Length   566 a.a.
NCBI ID   WP_193022219.1    Uniprot ID   A0A7L9S6F3
Organism   Thalassotalea sp. LPB0316     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 181093..192793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP316_RS00765 (LP316_00765) ampE 182451..183305 (-) 855 WP_193022215.1 regulatory signaling modulator protein AmpE -
  LP316_RS00770 (LP316_00770) ampD 183342..183893 (-) 552 WP_193022216.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LP316_RS00775 (LP316_00775) - 183943..184464 (+) 522 WP_193022217.1 TIGR02281 family clan AA aspartic protease -
  LP316_RS00780 (LP316_00780) nadC 184465..185328 (+) 864 WP_193022218.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LP316_RS16100 (LP316_00785) - 185547..185972 (+) 426 WP_413470664.1 pilin -
  LP316_RS00790 (LP316_00790) pilB 186093..187793 (+) 1701 WP_193022219.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LP316_RS00795 (LP316_00795) pilC 187862..189091 (+) 1230 WP_193022220.1 type II secretion system F family protein Machinery gene
  LP316_RS00800 (LP316_00800) pilD 189212..190099 (+) 888 WP_193022221.1 prepilin peptidase Machinery gene
  LP316_RS00805 (LP316_00805) coaE 190125..190754 (+) 630 WP_193022222.1 dephospho-CoA kinase -
  LP316_RS00810 (LP316_00810) zapD 190853..191599 (+) 747 WP_193022223.1 cell division protein ZapD -
  LP316_RS00815 (LP316_00815) yacG 191680..191916 (+) 237 WP_193022224.1 DNA gyrase inhibitor YacG -
  LP316_RS00820 (LP316_00820) mutT 192016..192402 (-) 387 WP_193022225.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62704.77 Da        Isoelectric Point: 4.9650

>NTDB_id=492060 LP316_RS00790 WP_193022219.1 186093..187793(+) (pilB) [Thalassotalea sp. LPB0316]
MKGLHQQSSLLSALAKHQLVSDEQLDEISSGFISQNKPFVRYLVEDKHLNASKVASIFSQSFGYPYIDLDAFDTSLIPEG
IRNEKLIRKHSALPIYLRGKVLFVAMSDPTNLDALEEIQFNTGYTTELILANENSLSTAIERILEEDTDALDISDMDTEE
LAGIDVEEERKDDTNEEGKDDAPIVVYINKILLDAIRKGASDLHFEPYEKAYRIRFRIDGILTEIARPPVTLSSRMAARL
KVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDMLGYEPEQKKIYMDALAQPQG
MILVTGPTGSGKTVSLYTGLNILNTEQRNISTAEDPVEINLEGINQVQINTRAGLTFPSALRSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSIIIAQRLARRLCMACKEPEHVPDEELIKQGFTH
EQLPNVKLFKAVGCDQCTGGYKGRVGIYEVIKIGEKLSSIIMEGGNSLDIAEQCQKEGFNNLRQSGLIKAMQGVTSLEEV
NRVTSG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=492060 LP316_RS00790 WP_193022219.1 186093..187793(+) (pilB) [Thalassotalea sp. LPB0316]
ATGAAAGGACTTCATCAACAATCAAGTTTGCTATCGGCACTTGCTAAGCATCAGCTGGTGTCAGACGAACAACTAGACGA
GATCAGCTCTGGTTTTATTAGCCAGAACAAACCTTTTGTCCGCTATTTAGTCGAAGACAAACACCTCAATGCCAGTAAAG
TAGCCAGTATTTTTTCCCAAAGTTTCGGCTACCCTTATATCGATCTAGATGCCTTTGATACAAGCCTTATACCCGAGGGT
ATTCGCAACGAAAAGCTGATCCGTAAACACAGCGCATTGCCAATTTACCTGCGCGGTAAAGTATTATTTGTGGCCATGTC
GGATCCAACTAACTTAGATGCGTTGGAAGAAATCCAGTTTAATACCGGTTATACCACTGAGCTTATTCTTGCCAATGAAA
ATAGCTTGTCTACCGCGATAGAGCGGATTTTAGAAGAAGATACTGATGCGCTCGATATTTCTGACATGGATACCGAAGAG
CTCGCCGGTATCGATGTCGAAGAAGAGCGCAAAGATGATACCAACGAAGAGGGTAAAGATGATGCCCCAATTGTTGTTTA
CATCAATAAAATCCTGCTCGATGCCATCAGAAAAGGCGCCTCGGATTTACACTTTGAGCCCTATGAGAAAGCTTATCGCA
TTCGCTTTCGTATCGACGGCATTTTAACTGAAATAGCGCGTCCGCCAGTGACCTTATCATCTAGGATGGCAGCGCGTTTG
AAAGTTATGTCAAAACTCGATATTGCTGAGCGCCGTGTGCCGCAAGATGGTCGAATCAAACTTGCGTTATCGAAAAAGAA
ATCAATCGACTTTCGCGTCAGTACCCTGCCCACCATGTGGGGCGAAAAAATTGTAATGCGTATTCTCGATAGCTCAAGTG
CCATGTTAGGCATTGACATGTTAGGCTATGAGCCAGAGCAAAAGAAAATCTACATGGACGCATTAGCTCAACCGCAAGGC
ATGATTCTGGTGACCGGCCCAACGGGTTCTGGTAAAACCGTATCGCTTTATACGGGTTTAAACATTCTCAATACCGAGCA
GCGCAATATATCGACAGCAGAAGATCCCGTTGAAATTAACCTAGAAGGTATCAACCAAGTACAAATCAATACCAGAGCTG
GGTTAACTTTCCCAAGCGCTTTGCGCTCATTTTTACGTCAAGATCCCGATATTGTCATGGTGGGTGAAATTCGAGATTTA
GAAACGGCGGAAATCGCAATAAAAGCGGCGCAAACAGGTCACTTAGTGTTATCGACACTACACACTAACTCGGCAGCAGA
AACGCTTACCCGTTTATTAAATATGGGCGTGCCCTCGTATAACGTCGCAAGCTCAGTCAGTATTATTATTGCTCAGCGAT
TAGCGCGACGCCTATGTATGGCTTGTAAAGAGCCTGAGCACGTTCCAGATGAAGAGCTGATCAAGCAAGGTTTTACCCAT
GAACAATTGCCAAACGTAAAGCTATTTAAAGCAGTCGGCTGTGATCAATGTACTGGCGGCTATAAAGGCCGAGTCGGTAT
TTACGAAGTGATCAAGATAGGTGAAAAGCTTTCTTCAATTATTATGGAAGGGGGCAATTCACTCGATATTGCCGAGCAAT
GTCAAAAGGAAGGTTTCAACAACTTGAGGCAATCAGGCTTAATCAAAGCAATGCAAGGTGTCACTAGCCTGGAAGAAGTC
AACCGAGTTACTAGCGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L9S6F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

61.77

93.816

0.58
  pilB Acinetobacter baumannii D1279779

61.174

93.286

0.571
  pilB Legionella pneumophila strain ERS1305867

57.627

93.816

0.541
  pilB Vibrio cholerae strain A1552

52.763

99.117

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

53.422

92.933

0.496
  pilB Vibrio campbellii strain DS40M4

52.652

93.286

0.491
  pilF Neisseria gonorrhoeae MS11

49.198

99.117

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.497

98.41

0.389