Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   LP316_RS00800 Genome accession   NZ_CP062946
Coordinates   189212..190099 (+) Length   295 a.a.
NCBI ID   WP_193022221.1    Uniprot ID   -
Organism   Thalassotalea sp. LPB0316     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 184212..195099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP316_RS00780 (LP316_00780) nadC 184465..185328 (+) 864 WP_193022218.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LP316_RS16100 (LP316_00785) - 185547..185972 (+) 426 WP_413470664.1 pilin -
  LP316_RS00790 (LP316_00790) pilB 186093..187793 (+) 1701 WP_193022219.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LP316_RS00795 (LP316_00795) pilC 187862..189091 (+) 1230 WP_193022220.1 type II secretion system F family protein Machinery gene
  LP316_RS00800 (LP316_00800) pilD 189212..190099 (+) 888 WP_193022221.1 prepilin peptidase Machinery gene
  LP316_RS00805 (LP316_00805) coaE 190125..190754 (+) 630 WP_193022222.1 dephospho-CoA kinase -
  LP316_RS00810 (LP316_00810) zapD 190853..191599 (+) 747 WP_193022223.1 cell division protein ZapD -
  LP316_RS00815 (LP316_00815) yacG 191680..191916 (+) 237 WP_193022224.1 DNA gyrase inhibitor YacG -
  LP316_RS00820 (LP316_00820) mutT 192016..192402 (-) 387 WP_193022225.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 295 a.a.        Molecular weight: 33744.07 Da        Isoelectric Point: 6.9905

>NTDB_id=492062 LP316_RS00800 WP_193022221.1 189212..190099(+) (pilD) [Thalassotalea sp. LPB0316]
MFADIFALFEQSPSYFYTTVFIFSLMVGSFLNVVIYRFPKMLEREWYRDCREFLADEVKDIPEKQFDTLTLSKPASSCPK
CHHKIRFYENIPVISWLLLKGKCSQCQTAISARYPIIELSTAILSVAVACFFGPTMTTLWVILLTWFLICLTMIDFDHML
LPDQLVYPMLWLGLLVNINGQFATLSDAVIGAVAGYMSLYSVFWLFKLLTGKEGMGHGDFKLVAMFGAWFGWQLLPLHIL
MASVVAAIVGISLIAFKQHNRENPIPFGPYLAVAGWICLLWGNDIWRWYLGYIMA

Nucleotide


Download         Length: 888 bp        

>NTDB_id=492062 LP316_RS00800 WP_193022221.1 189212..190099(+) (pilD) [Thalassotalea sp. LPB0316]
ATGTTTGCAGACATTTTCGCCCTTTTTGAACAATCACCAAGTTACTTTTACACCACCGTATTTATCTTCTCTCTTATGGT
TGGTAGTTTTTTAAATGTGGTTATTTATCGCTTTCCTAAAATGCTAGAGCGCGAATGGTACCGCGATTGCAGAGAATTCT
TAGCTGATGAAGTTAAGGATATACCCGAAAAGCAATTTGACACACTGACCTTGTCAAAGCCAGCGTCAAGTTGTCCTAAA
TGTCATCACAAAATTCGCTTCTACGAGAATATTCCTGTTATTAGCTGGCTGTTGCTCAAAGGTAAATGTAGCCAATGCCA
AACTGCAATTTCTGCGCGTTACCCCATCATAGAACTTAGTACGGCAATACTTAGTGTCGCTGTGGCCTGTTTTTTCGGAC
CTACGATGACCACCCTTTGGGTTATTTTACTGACGTGGTTTTTGATCTGTTTAACTATGATTGACTTTGACCACATGTTA
CTGCCGGATCAGTTAGTTTATCCGATGCTGTGGCTTGGTTTACTGGTTAATATTAACGGTCAATTTGCTACCTTGTCAGA
CGCCGTAATTGGTGCTGTAGCTGGCTATATGAGCCTTTATTCTGTGTTTTGGTTATTCAAATTATTAACCGGTAAAGAAG
GTATGGGACATGGCGATTTCAAACTCGTTGCCATGTTTGGCGCTTGGTTTGGTTGGCAACTTTTACCCCTTCACATCCTT
ATGGCCTCTGTCGTTGCAGCAATTGTTGGCATCAGTCTGATTGCCTTTAAACAACACAACCGAGAAAACCCGATTCCATT
TGGCCCATATCTCGCTGTAGCCGGCTGGATTTGCCTGTTATGGGGTAACGACATTTGGCGATGGTACTTAGGTTATATAA
TGGCTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-131)

Type IV leader peptidase family (Peptidase_A24)

(143-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

52.595

97.966

0.515
  pilD Acinetobacter nosocomialis M2

50

97.627

0.488
  pilD Acinetobacter baumannii D1279779

50

97.627

0.488
  pilD Vibrio cholerae strain A1552

50.89

95.254

0.485
  pilD Neisseria gonorrhoeae MS11

47.368

90.169

0.427