Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F7R26_RS17910 Genome accession   NZ_CP062803
Coordinates   3898025..3899770 (+) Length   581 a.a.
NCBI ID   WP_150986824.1    Uniprot ID   A0A643FSW4
Organism   Cupriavidus basilensis strain DSM 11853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3893025..3904770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7R26_RS17875 (F7R26_017870) proB 3893029..3894147 (-) 1119 WP_043349741.1 glutamate 5-kinase -
  F7R26_RS17880 (F7R26_017875) obgE 3894187..3895287 (-) 1101 WP_150986825.1 GTPase ObgE -
  F7R26_RS17890 (F7R26_017880) rpmA 3895437..3895697 (-) 261 WP_006162509.1 50S ribosomal protein L27 -
  F7R26_RS17895 (F7R26_017885) rplU 3895768..3896079 (-) 312 WP_006162510.1 50S ribosomal protein L21 -
  F7R26_RS17900 (F7R26_017890) ispB 3896652..3897581 (+) 930 WP_150986886.1 octaprenyl diphosphate synthase -
  F7R26_RS17910 (F7R26_017900) pilF 3898025..3899770 (+) 1746 WP_150986824.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F7R26_RS17915 (F7R26_017905) - 3899801..3901066 (+) 1266 WP_150986823.1 type II secretion system F family protein -
  F7R26_RS17920 (F7R26_017910) pilD 3901070..3901987 (+) 918 WP_241754358.1 prepilin peptidase Machinery gene
  F7R26_RS17925 (F7R26_017915) coaE 3902055..3902666 (+) 612 WP_150986822.1 dephospho-CoA kinase -
  F7R26_RS17930 (F7R26_017920) zapD 3902941..3903699 (+) 759 WP_150986821.1 cell division protein ZapD -
  F7R26_RS17935 (F7R26_017925) - 3903704..3903889 (+) 186 WP_150986820.1 DNA gyrase inhibitor YacG -
  F7R26_RS17940 (F7R26_017930) - 3903938..3904378 (-) 441 WP_150986819.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63896.47 Da        Isoelectric Point: 7.0952

>NTDB_id=491485 F7R26_RS17910 WP_150986824.1 3898025..3899770(+) (pilF) [Cupriavidus basilensis strain DSM 11853]
MTLGLALAQSRRIAPALLAQLEQSAREKKSQLIDEIIASGTMTAHDVAIFAADKYQLPLIDLNQYNLNKVPPALAGNREF
HAHRLLPLGRRENRLVLALSDPGNQAGLDAIKQKYNLPVEPVVVEHDKLMQYVRSASDDSGGTIKSIGPGHVERKMIDYD
PVASAGALRNKTAADIDDAPVVRFLHKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPVDIRDKISTRIKVL
SRLDISEKRVPQDGRMKLLIALPKEKGGDKDGKEPKETVEKAIDFRVSTLPTLFGEKIVIRILESSSDKLDIDQLGYEPA
QKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNEKAGLTFSAALRSFLR
QDPDIIMVGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCTCKRPGE
LAREVLADAGFQDEDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEPMQQIILAHGTALEIAEQARKDGVLSLREA
GLAKVKQGLTSLEEVLATTNM

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=491485 F7R26_RS17910 WP_150986824.1 3898025..3899770(+) (pilF) [Cupriavidus basilensis strain DSM 11853]
ATGACACTTGGTCTAGCTCTTGCACAGAGCCGGCGTATCGCGCCGGCCCTGCTCGCCCAGCTTGAACAGTCGGCACGAGA
AAAAAAGTCGCAGCTGATCGACGAGATCATCGCCAGCGGCACCATGACTGCACATGATGTGGCGATCTTCGCGGCTGATA
AGTATCAGCTGCCGCTGATCGATCTGAATCAGTACAACCTCAACAAGGTGCCGCCGGCGCTGGCGGGCAATCGCGAGTTC
CACGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAGAACCGCCTGGTGCTGGCGTTGTCCGACCCGGGCAACCAGGCCGG
GCTGGACGCCATCAAGCAGAAATACAACCTGCCGGTGGAACCCGTCGTGGTCGAGCACGACAAGCTGATGCAATACGTGC
GCTCGGCTTCCGACGACAGCGGCGGCACGATCAAGTCGATCGGCCCGGGGCATGTCGAGCGCAAGATGATCGACTACGAC
CCGGTGGCGTCCGCCGGGGCGCTGCGCAACAAGACCGCGGCCGACATCGACGACGCGCCCGTGGTCCGCTTCCTGCACAA
GCTGCTGACCGAAGCCTTCCATCGCGGCGCCTCCGATCTTCACTTCGAGCCGTTCGAGACCTTCTACCGCATCCGCTTCC
GGGTGGATGGCGTGCTGCAGGAAGTGGCACGGCCGCCGGTGGACATCCGGGACAAGATCTCCACCCGGATCAAGGTGCTG
TCGCGCCTGGACATCTCGGAAAAGCGCGTGCCGCAAGATGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGAAAAGGG
CGGCGACAAAGACGGCAAGGAGCCCAAGGAAACCGTGGAGAAGGCGATCGACTTCCGCGTCTCCACGCTGCCGACGCTGT
TCGGCGAGAAGATCGTGATCCGGATCCTGGAGTCGTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTACGAGCCGGCG
CAAAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGAC
GGTGTCGCTGTACACCTTCCTGAACCGGCTGAACCAGGGCGACATCAATATCTCCACCGCCGAGGATCCGGCGGAAATCC
AGTTGCCCGGCATCAACCAGGTCAACGTCAACGAAAAAGCCGGCCTGACCTTCTCCGCCGCGCTGCGCTCCTTCCTGCGC
CAGGATCCTGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCGGCACAGACTGG
CCACCTGGTGTTCTCGACGCTGCATACCAACGACGCGCCGACCACGCTCACGCGCCTGATGAACATGGGTGTCGCGCCCT
TCAATATCGCGTCGAGCGTGCTGATGATCACCGCGCAGCGGCTTGCGCGGCGGCTGTGCACCTGCAAGCGCCCCGGCGAG
CTGGCGCGCGAGGTGCTGGCCGACGCCGGCTTCCAGGACGAGGACCTCGACGGCAGCTGGCAGCCTTACCACCCGGTCGG
GTGCGAGCGCTGCAACGGCAGCGGCTACAAGGGCCGCTGCGGCATCTACCAGGTGATGCCGATCACCGAGCCGATGCAGC
AGATCATCCTCGCGCATGGCACGGCACTGGAGATCGCCGAACAAGCGCGTAAAGATGGCGTGCTATCGTTGCGCGAAGCT
GGCCTGGCCAAGGTGAAGCAGGGTCTCACCTCGCTCGAAGAAGTGCTGGCCACAACAAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-471)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A643FSW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.424

100

0.511
  pilB Acinetobacter baumannii D1279779

48.649

100

0.496
  pilB Acinetobacter baylyi ADP1

49.293

97.418

0.48
  pilB Legionella pneumophila strain ERS1305867

47.434

97.246

0.461
  pilB Vibrio cholerae strain A1552

43.966

99.828

0.439
  pilB Vibrio parahaemolyticus RIMD 2210633

45.57

95.181

0.434
  pilB Vibrio campbellii strain DS40M4

44.909

94.664

0.425
  pilF Thermus thermophilus HB27

37.694

100

0.377