Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   STH8232_RS02065 Genome accession   NC_017581
Coordinates   376758..378077 (+) Length   439 a.a.
NCBI ID   WP_014621181.1    Uniprot ID   -
Organism   Streptococcus thermophilus JIM 8232     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 371758..383077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STH8232_RS02035 (STH8232_0462) - 372613..373377 (+) 765 WP_002949740.1 ABC transporter ATP-binding protein -
  STH8232_RS02040 (STH8232_0463) - 373377..374087 (+) 711 WP_014621177.1 ABC transporter ATP-binding protein -
  STH8232_RS10265 - 374223..374883 (+) 661 Protein_350 CBS and ACT domain-containing protein -
  STH8232_RS02055 (STH8232_0466) cysK 375049..375975 (-) 927 WP_014621180.1 cysteine synthase A -
  STH8232_RS02060 (STH8232_0467) - 376077..376703 (-) 627 WP_011680769.1 YigZ family protein -
  STH8232_RS02065 (STH8232_0468) comFA/cflA 376758..378077 (+) 1320 WP_014621181.1 DEAD/DEAH box helicase Machinery gene
  STH8232_RS02070 (STH8232_0469) - 378058..378720 (+) 663 WP_014621182.1 ComF family protein -
  STH8232_RS02075 (STH8232_0470) hpf 378799..379347 (+) 549 WP_014621183.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50612.75 Da        Isoelectric Point: 9.7105

>NTDB_id=48981 STH8232_RS02065 WP_014621181.1 376758..378077(+) (comFA/cflA) [Streptococcus thermophilus JIM 8232]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEIIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPRFFWQGRLQKSLTNEKLPRSLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFPQVDVFVCMANHHLYTSSSLIQIGGRVGRSPER
PIGKLYFFHEGLSKSMLQCRKEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=48981 STH8232_RS02065 WP_014621181.1 376758..378077(+) (comFA/cflA) [Streptococcus thermophilus JIM 8232]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGACTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATAATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGATGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAACGAGAAGCTTCCAAGGTCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTTCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTATAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGAAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

54.756

98.178

0.538
  comFA/cflA Streptococcus pneumoniae Rx1

54.756

98.178

0.538
  comFA/cflA Streptococcus pneumoniae D39

54.756

98.178

0.538
  comFA/cflA Streptococcus pneumoniae R6

54.756

98.178

0.538
  comFA/cflA Streptococcus mitis NCTC 12261

54.419

97.95

0.533
  comFA/cflA Streptococcus mitis SK321

53.488

97.95

0.524
  comFA Lactococcus lactis subsp. cremoris KW2

45.476

95.672

0.435
  comFA Latilactobacillus sakei subsp. sakei 23K

36.782

99.089

0.364

Multiple sequence alignment