Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   KFU64_RS22435 Genome accession   NZ_CP073976
Coordinates   4615259..4616779 (+) Length   506 a.a.
NCBI ID   WP_000058964.1    Uniprot ID   P22787
Organism   Escherichia coli strain MB20     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4610259..4621779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU64_RS22410 (KFU64_22215) ilvE 4611813..4612742 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  KFU64_RS22415 (KFU64_22220) ilvM 4612762..4613025 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  KFU64_RS22420 (KFU64_22225) ilvG 4613022..4614668 (-) 1647 WP_001012608.1 acetolactate synthase 2 catalytic subunit -
  KFU64_RS22425 (KFU64_22230) ilvX 4614671..4614721 (-) 51 WP_001387183.1 peptide IlvX -
  KFU64_RS22430 (KFU64_22235) ilvL 4614808..4614906 (-) 99 WP_001311244.1 ilv operon leader peptide -
  KFU64_RS22435 (KFU64_22240) comM 4615259..4616779 (+) 1521 WP_000058964.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  KFU64_RS22440 (KFU64_22245) maoP 4616804..4617142 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  KFU64_RS22445 (KFU64_22250) hdfR 4617261..4618100 (+) 840 WP_000379245.1 HTH-type transcriptional regulator HdfR -
  KFU64_RS22450 (KFU64_22255) tnpA 4618270..4618728 (-) 459 WP_257182458.1 IS200/IS605-like element IS200C family transposase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55146.35 Da        Isoelectric Point: 7.6191

>NTDB_id=489214 KFU64_RS22435 WP_000058964.1 4615259..4616779(+) (comM) [Escherichia coli strain MB20]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=489214 KFU64_RS22435 WP_000058964.1 4615259..4616779(+) (comM) [Escherichia coli strain MB20]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCGATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTGGCTCCAGCTGATCTGCCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGTGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCATCGTTAACTGCGATGGTAGGCGGTGGCGCAATTCCAGGGCCCGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATA
A

Domains

Predicted by InterProScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P22787

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.904

100

0.595
  comM Vibrio cholerae O1 biovar El Tor strain E7946

59.562

99.209

0.591
  comM Vibrio cholerae strain A1552

59.562

99.209

0.591
  comM Glaesserella parasuis strain SC1401

58.777

100

0.589
  comM Vibrio campbellii strain DS40M4

58.367

99.209

0.579
  comM Acinetobacter baylyi ADP1

48.996

98.419

0.482
  comM Legionella pneumophila str. Paris

47.992

98.419

0.472
  comM Legionella pneumophila strain ERS1305867

47.992

98.419

0.472
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443