Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KCG51_RS09450 Genome accession   NZ_CP073119
Coordinates   1998860..1999318 (+) Length   152 a.a.
NCBI ID   WP_254342475.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1993860..2004318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG51_RS09435 (KCG51_09465) - 1994595..1994891 (+) 297 WP_003684318.1 helix-turn-helix transcriptional regulator -
  KCG51_RS09440 (KCG51_09470) glnD 1994911..1997468 (+) 2558 Protein_1825 [protein-PII] uridylyltransferase -
  KCG51_RS09445 (KCG51_09475) pilE 1998262..1998759 (+) 498 WP_254342474.1 pilin Machinery gene
  KCG51_RS09450 (KCG51_09480) pilE 1998860..1999318 (+) 459 WP_254342475.1 pilin Machinery gene
  KCG51_RS09455 (KCG51_09485) - 1999530..2001055 (-) 1526 Protein_1828 DHA2 family efflux MFS transporter permease subunit -
  KCG51_RS09460 (KCG51_09490) - 2001157..2002347 (-) 1191 WP_254342476.1 efflux RND transporter periplasmic adaptor subunit -
  KCG51_RS09465 (KCG51_09495) - 2002572..2003978 (-) 1407 WP_254342478.1 efflux transporter outer membrane subunit -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16195.88 Da        Isoelectric Point: 8.9487

>NTDB_id=487085 KCG51_RS09450 WP_254342475.1 1998860..1999318(+) (pilE) [Neisseria subflava strain HP0069]
MKKLRKAQTGFSLIELMIVIGIIGILAVAALPAYQNYVARAQVAEGIMLAGSMKSYVEEAYADKGSLPLDLNIKQLASDD
NKVGNYVKIVRIEDNGTIVAAIGNQAAVPVKNTEIYLTPSFAKTGATKGTTVWSCGGTMDTHFLPVSCKQSL

Nucleotide


Download         Length: 459 bp        

>NTDB_id=487085 KCG51_RS09450 WP_254342475.1 1998860..1999318(+) (pilE) [Neisseria subflava strain HP0069]
ATGAAAAAACTACGCAAAGCCCAAACCGGTTTTAGCCTGATTGAGCTGATGATTGTTATCGGCATCATTGGCATTTTGGC
AGTAGCAGCTCTTCCTGCATACCAAAACTACGTTGCCAGAGCACAAGTAGCAGAAGGCATCATGCTGGCAGGCAGTATGA
AAAGCTACGTTGAAGAAGCCTATGCAGATAAAGGCAGCTTGCCGCTTGACCTCAATATCAAGCAACTCGCTTCAGACGAC
AATAAAGTCGGTAACTATGTAAAGATTGTCCGTATTGAAGATAATGGCACCATTGTTGCCGCCATCGGTAATCAAGCCGC
TGTACCTGTGAAGAATACCGAAATCTACCTGACACCAAGTTTTGCAAAAACAGGGGCTACTAAAGGTACAACAGTTTGGT
CTTGCGGTGGCACCATGGATACACACTTCCTTCCCGTATCCTGCAAACAAAGCTTGTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)

Pilin (bacterial filament) (Pilin)

(39-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

38.994

100

0.408
  pilE Neisseria gonorrhoeae MS11

38.125

100

0.401
  pilA Ralstonia pseudosolanacearum GMI1000

32.571

100

0.375
  pilA2 Legionella pneumophila str. Paris

37.584

98.026

0.368
  pilA2 Legionella pneumophila strain ERS1305867

37.584

98.026

0.368
  pilA Haemophilus influenzae 86-028NP

36.667

98.684

0.362