Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IC628_RS02325 Genome accession   NZ_CP061861
Coordinates   475900..477006 (+) Length   368 a.a.
NCBI ID   WP_086957245.1    Uniprot ID   A0A1V1V6D8
Organism   Photobacterium damselae subsp. piscicida strain AS-16-0555-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470900..482006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC628_RS02295 (IC628_02295) - 471736..472329 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  IC628_RS02300 (IC628_02300) yggU 472431..472730 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  IC628_RS02305 (IC628_02305) - 472727..473284 (-) 558 WP_005297682.1 YggT family protein -
  IC628_RS02310 (IC628_02310) proC 473296..474114 (-) 819 WP_086957247.1 pyrroline-5-carboxylate reductase -
  IC628_RS02315 (IC628_02315) - 474130..474831 (-) 702 WP_086957246.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC628_RS02320 (IC628_02320) - 474854..475888 (+) 1035 Protein_431 type IV pilus twitching motility protein PilT -
  IC628_RS02325 (IC628_02325) pilU 475900..477006 (+) 1107 WP_086957245.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC628_RS02330 (IC628_02330) ruvX 477334..477759 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  IC628_RS02335 (IC628_02335) - 477756..478319 (-) 564 WP_044174430.1 YqgE/AlgH family protein -
  IC628_RS02340 (IC628_02340) gshB 478473..479420 (-) 948 WP_086957244.1 glutathione synthase -
  IC628_RS02345 (IC628_02345) rsmE 479441..480172 (-) 732 WP_044174434.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IC628_RS02350 (IC628_02350) endA 480304..481008 (-) 705 WP_086957243.1 deoxyribonuclease I -
  IC628_RS02355 (IC628_02355) - 481139..481444 (-) 306 Protein_438 SprT-like domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41368.53 Da        Isoelectric Point: 5.5903

>NTDB_id=485332 IC628_RS02325 WP_086957245.1 475900..477006(+) (pilU) [Photobacterium damselae subsp. piscicida strain AS-16-0555-7]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGETLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=485332 IC628_RS02325 WP_086957245.1 475900..477006(+) (pilU) [Photobacterium damselae subsp. piscicida strain AS-16-0555-7]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGAGTGTTGCATCCCATTGGCGAGACGTTAGATCGTACTGGGGTTGATTTGCTATTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTACGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGGCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACGAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTTACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V1V6D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.392

99.728

0.682
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.739

93.75

0.391
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367