Detailed information NTG0485220

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAINH2_RS12065
• Genome accession: NZ_CP061852
• Coordinates: 2537414..2538028 (-)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain INH2-4b
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2532414..2543028

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAINH2_RS12035 (BAINH2_12035)	rpsT	2532714..2532980 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAINH2_RS12040 (BAINH2_12040)	holA	2532997..2534038 (-)	1042	Protein_2341	DNA polymerase III subunit delta	-
BAINH2_RS12045 (BAINH2_12045)	-	2534078..2534232 (-)	155	Protein_2342	hypothetical protein	-
BAINH2_RS12050 (BAINH2_12050)	-	2534273..2534407 (+)	135	WP_003152870.1	YqzM family protein	-
BAINH2_RS12055 (BAINH2_12055)	comEC	2534426..2536777 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAINH2_RS12060 (BAINH2_12060)	-	2536778..2537347 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAINH2_RS12065 (BAINH2_12065)	comEA	2537414..2538028 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAINH2_RS12070 (BAINH2_12070)	comER	2538087..2538908 (+)	822	WP_015417855.1	late competence protein ComER	-
BAINH2_RS12075 (BAINH2_12075)	-	2538977..2539714 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
BAINH2_RS12080 (BAINH2_12080)	rsfS	2539711..2540067 (-)	357	WP_007408260.1	ribosome silencing factor	-
BAINH2_RS12085 (BAINH2_12085)	yqeK	2540085..2540645 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAINH2_RS12090 (BAINH2_12090)	-	2540635..2541204 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BAINH2_RS12095 (BAINH2_12095)	yhbY	2541215..2541505 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAINH2_RS12100 (BAINH2_12100)	aroE	2541499..2542341 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=485220 BAINH2_RS12065 WP_053573180.1 2537414..2538028(-) (comEA) [Bacillus amyloliquefaciens strain INH2-4b]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=485220 BAINH2_RS12065 WP_053573180.1 2537414..2538028(-) (comEA) [Bacillus amyloliquefaciens strain INH2-4b]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363