Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2514_RS01960 Genome accession   NZ_CP061847
Coordinates   468137..469864 (-) Length   575 a.a.
NCBI ID   WP_036191997.1    Uniprot ID   A0A0A0ERC5
Organism   Lysobacter sp. CW239     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463137..474864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2514_RS01940 (H2514_01940) mutT 463353..464396 (+) 1044 WP_036191987.1 8-oxo-dGTP diphosphatase MutT -
  H2514_RS01945 (H2514_01945) coaE 464858..465496 (-) 639 WP_036191989.1 dephospho-CoA kinase -
  H2514_RS01950 (H2514_01950) pilD 465635..466498 (-) 864 WP_036191991.1 A24 family peptidase Machinery gene
  H2514_RS01955 (H2514_01955) pilC 466558..467820 (-) 1263 WP_036191993.1 type II secretion system F family protein Machinery gene
  H2514_RS01960 (H2514_01960) pilB 468137..469864 (-) 1728 WP_036191997.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2514_RS01965 (H2514_01965) - 470143..470424 (+) 282 WP_036192000.1 type II toxin-antitoxin system RelE/ParE family toxin -
  H2514_RS01970 (H2514_01970) - 470434..470730 (+) 297 WP_036192002.1 HigA family addiction module antitoxin -
  H2514_RS01975 (H2514_01975) - 470776..471210 (-) 435 WP_224743902.1 DUF1643 domain-containing protein -
  H2514_RS13540 - 471233..471367 (-) 135 WP_255516726.1 hypothetical protein -
  H2514_RS13545 (H2514_01980) - 471556..472014 (-) 459 WP_036192005.1 pilin -
  H2514_RS01985 (H2514_01985) - 472450..472728 (+) 279 WP_036192015.1 type II toxin-antitoxin system RelE/ParE family toxin -
  H2514_RS01990 (H2514_01990) - 472747..473028 (+) 282 WP_036192407.1 HigA family addiction module antitoxin -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62816.04 Da        Isoelectric Point: 5.7574

>NTDB_id=485075 H2514_RS01960 WP_036191997.1 468137..469864(-) (pilB) [Lysobacter sp. CW239]
MNAVATPNLMGITGIARRLVQDGALTEASAREAMAGATDARKPLATYLTEKRLVAPAHLAAANSIEFGVPLFDAAAMDPE
QSAVKLVNEELLRKHRVLPLFRRGNRLFVGIAEPTNTHALSEIKFQTNFTVEAILVDEESIKRHLDRWLENADALGDAMG
GDDEGLENLDVGGDDDLSSDDGVEAKTDDTPVVKFINKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQAARVPVKL
QARISARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDDQRDLFL
AAVKRPYGMVLVTGPTGSGKTVSLYTALNILNDETRNISTVEDPVEIRVPGINQVQMNPKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVSLVIAQRLARRLHDCKRPVELPEHAL
LAEGFTAEEIAAPEFRIYEAVGCADCTEGYKGRTGVYQVMPMSDEIQAIVLAGGNAMQIAEAAQAAGVRDLRQSALMKVR
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=485075 H2514_RS01960 WP_036191997.1 468137..469864(-) (pilB) [Lysobacter sp. CW239]
ATGAACGCTGTCGCCACTCCCAACCTGATGGGCATCACCGGTATCGCCCGCCGTCTGGTGCAGGACGGGGCGCTGACCGA
GGCCTCTGCGCGCGAGGCGATGGCCGGGGCGACGGACGCGCGCAAGCCGTTGGCGACCTACCTCACCGAGAAGCGGTTGG
TCGCGCCGGCGCATCTTGCCGCCGCCAATTCGATCGAGTTCGGGGTGCCGTTGTTCGATGCGGCGGCGATGGACCCGGAG
CAGTCGGCGGTCAAGCTGGTCAACGAGGAGCTGCTGCGCAAGCACCGCGTGCTGCCGTTGTTCCGGCGCGGTAACCGGTT
GTTTGTGGGCATTGCCGAGCCAACCAACACCCACGCGTTGTCGGAGATCAAGTTCCAGACCAACTTCACGGTCGAGGCGA
TCCTGGTCGACGAGGAGAGCATCAAGCGCCACCTGGACCGCTGGCTGGAAAACGCGGATGCTCTCGGCGATGCGATGGGC
GGGGACGACGAGGGGCTGGAGAACCTCGATGTCGGCGGCGACGACGACCTGTCCTCCGACGATGGTGTCGAAGCCAAGAC
CGACGACACGCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTCGACGCGATTCGCCGCGGTGCGTCGGACATCCACTTCG
AGCCTTACGAGACCGAATACCGGGTGCGGCTGCGCATCGACGGTCTGCTGAAGCAGGCGGCACGGGTGCCGGTGAAGTTG
CAGGCCCGTATTTCCGCGCGCTTGAAGGTGATGGCGCAGTTGGACATCGCCGAGAAACGGGTGCCGCAGGACGGCCGCAT
CAAGCTCAACCTGAGCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCCTGCCGACGCTGTTCGGCGAGAAGATCGTGC
TGCGTATCCTGGACGGCAGCGCGGCCAGGCTGGGCATCGAGAAGCTCGGTTACGAGGACGACCAGCGCGACCTGTTCCTG
GCGGCGGTGAAGCGGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGTTGTACACCGC
GCTGAACATCCTCAACGACGAGACCCGCAACATCTCCACGGTCGAGGACCCGGTGGAAATCCGCGTGCCGGGCATCAACC
AGGTGCAGATGAACCCCAAACGCGGCATGACGTTTGCCGCGGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGACGTGATC
ATGGTCGGCGAGATCCGCGACCTGGAAACCGCCGAGATCGGCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCGAC
CCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCACCTCGTCGG
TGTCACTGGTGATCGCCCAGCGCCTGGCGCGACGGCTGCACGACTGCAAGCGGCCGGTGGAGCTGCCGGAGCATGCGCTG
CTGGCCGAAGGCTTCACCGCCGAGGAGATCGCGGCGCCGGAGTTCCGGATCTACGAAGCCGTGGGCTGCGCGGACTGCAC
CGAAGGCTACAAGGGCCGTACCGGCGTGTACCAGGTGATGCCGATGAGCGACGAGATCCAGGCCATCGTGCTGGCCGGCG
GCAACGCGATGCAGATTGCCGAGGCCGCACAAGCCGCCGGCGTGCGCGACCTGCGCCAGTCCGCACTGATGAAGGTGCGC
AACGGGGTGACCAGCCTGGCGGAGATCAACCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A0ERC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.844

100

0.551
  pilB Acinetobacter baumannii D1279779

55.3

98.435

0.544
  pilB Legionella pneumophila strain ERS1305867

50.088

98.609

0.494
  pilF Neisseria gonorrhoeae MS11

47.88

98.435

0.471
  pilB Vibrio cholerae strain A1552

52.437

89.217

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

51.724

85.739

0.443
  pilB Vibrio campbellii strain DS40M4

50.909

86.087

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.288

100

0.377