Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H2514_RS01950 Genome accession   NZ_CP061847
Coordinates   465635..466498 (-) Length   287 a.a.
NCBI ID   WP_036191991.1    Uniprot ID   A0A0A0EV35
Organism   Lysobacter sp. CW239     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 460635..471498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2514_RS01940 (H2514_01940) mutT 463353..464396 (+) 1044 WP_036191987.1 8-oxo-dGTP diphosphatase MutT -
  H2514_RS01945 (H2514_01945) coaE 464858..465496 (-) 639 WP_036191989.1 dephospho-CoA kinase -
  H2514_RS01950 (H2514_01950) pilD 465635..466498 (-) 864 WP_036191991.1 A24 family peptidase Machinery gene
  H2514_RS01955 (H2514_01955) pilC 466558..467820 (-) 1263 WP_036191993.1 type II secretion system F family protein Machinery gene
  H2514_RS01960 (H2514_01960) pilB 468137..469864 (-) 1728 WP_036191997.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2514_RS01965 (H2514_01965) - 470143..470424 (+) 282 WP_036192000.1 type II toxin-antitoxin system RelE/ParE family toxin -
  H2514_RS01970 (H2514_01970) - 470434..470730 (+) 297 WP_036192002.1 HigA family addiction module antitoxin -
  H2514_RS01975 (H2514_01975) - 470776..471210 (-) 435 WP_224743902.1 DUF1643 domain-containing protein -
  H2514_RS13540 - 471233..471367 (-) 135 WP_255516726.1 hypothetical protein -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31697.49 Da        Isoelectric Point: 8.3317

>NTDB_id=485073 H2514_RS01950 WP_036191991.1 465635..466498(-) (pilD) [Lysobacter sp. CW239]
MAFLDQNPEIGYPLVAGFGLLLGSFLNVVILRLPKRMEWQWQRDSREVLGQPDDYDPPPPGIVVERSHCPHCKHQLSWYE
NIPVFSWLALRGRCRNCKARISVQYPLVELLTAVLMVACVARFGFGWQGFGAMVFTGFLISLSGIDLRTQLLPDQLTLPL
MWLGLIAAVDNLYMPAKPALIGAVVGYLSLWVVWWLFKQITGKEGMGHGDFKLLAAIGAWTGLSGILPTVLLSSLVGAII
GSIWLAAKGRDKATPIPFGPYLAVAGWIVFFWGEQMVDGYLRLAGLG

Nucleotide


Download         Length: 864 bp        

>NTDB_id=485073 H2514_RS01950 WP_036191991.1 465635..466498(-) (pilD) [Lysobacter sp. CW239]
ATGGCATTCCTCGACCAGAACCCCGAAATCGGCTACCCGCTGGTGGCCGGTTTCGGCCTGCTGCTGGGCAGCTTCCTGAA
CGTGGTGATCCTGCGCCTGCCCAAGCGGATGGAGTGGCAGTGGCAGCGCGACAGCCGCGAAGTGCTGGGCCAGCCGGACG
ATTACGATCCGCCGCCGCCGGGCATCGTGGTGGAGCGATCGCACTGTCCGCACTGCAAGCACCAGTTGTCGTGGTACGAG
AACATCCCGGTGTTCAGCTGGCTGGCCCTGCGCGGGCGCTGCCGCAACTGCAAGGCGCGGATCTCCGTGCAGTACCCGCT
GGTGGAGCTGCTGACCGCGGTGCTGATGGTTGCCTGCGTGGCGCGCTTCGGCTTCGGCTGGCAGGGCTTCGGGGCGATGG
TGTTCACCGGCTTCCTGATCTCGCTGTCGGGCATCGACCTGCGCACCCAGTTGCTGCCCGACCAGCTCACCCTGCCGTTG
ATGTGGCTGGGCCTGATCGCCGCGGTCGACAACCTGTACATGCCGGCCAAACCGGCGCTGATCGGCGCGGTGGTCGGCTA
CCTGAGCCTGTGGGTGGTGTGGTGGCTGTTCAAGCAGATCACCGGCAAGGAAGGCATGGGCCACGGCGACTTCAAGCTGC
TGGCCGCAATCGGCGCCTGGACCGGGCTGTCGGGCATCCTGCCGACGGTGCTGCTGTCGTCGCTGGTCGGGGCGATCATC
GGCTCGATCTGGCTGGCCGCGAAGGGCCGCGACAAGGCCACGCCGATCCCGTTCGGCCCCTACCTCGCCGTCGCTGGCTG
GATCGTGTTCTTCTGGGGCGAGCAGATGGTGGACGGGTATCTGCGGCTTGCCGGACTGGGCTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(133-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A0EV35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

48.611

100

0.488
  pilD Acinetobacter nosocomialis M2

47.569

100

0.477
  pilD Vibrio cholerae strain A1552

48.727

95.819

0.467
  pilD Vibrio campbellii strain DS40M4

49.442

93.728

0.463
  pilD Neisseria gonorrhoeae MS11

45.387

94.425

0.429