Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IF130_RS07220 Genome accession   NZ_CP061840
Coordinates   1594261..1595985 (+) Length   574 a.a.
NCBI ID   WP_011213804.1    Uniprot ID   A0A3A6VPZ0
Organism   Legionella pneumophila subsp. pneumophila strain AW-13-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1589261..1600985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF130_RS07200 (IF130_07200) - 1589608..1592136 (+) 2529 WP_011213800.1 EAL domain-containing protein -
  IF130_RS07205 (IF130_07205) - 1592138..1592707 (-) 570 WP_011213801.1 hypoxanthine-guanine phosphoribosyltransferase -
  IF130_RS07210 (IF130_07210) - 1592750..1593076 (-) 327 WP_011213802.1 DUF1820 family protein -
  IF130_RS07215 (IF130_07215) - 1593259..1594002 (-) 744 WP_011213803.1 methyltransferase domain-containing protein -
  IF130_RS07220 (IF130_07220) pilB 1594261..1595985 (+) 1725 WP_011213804.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IF130_RS07225 (IF130_07225) pilC 1595991..1597211 (+) 1221 WP_011213805.1 type II secretion system F family protein Machinery gene
  IF130_RS07230 (IF130_07230) pilD 1597257..1598120 (+) 864 WP_011213806.1 A24 family peptidase Machinery gene
  IF130_RS07235 (IF130_07235) - 1598134..1599339 (-) 1206 WP_011213807.1 cyclic nucleotide-binding domain-containing protein -
  IF130_RS07240 (IF130_07240) - 1599447..1600040 (-) 594 WP_011213808.1 hypothetical protein -
  IF130_RS07245 (IF130_07245) - 1600056..1600511 (-) 456 WP_011213809.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62676.28 Da        Isoelectric Point: 5.5880

>NTDB_id=484970 IF130_RS07220 WP_011213804.1 1594261..1595985(+) (pilB) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGAI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVEADKLSALIENLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=484970 IF130_RS07220 WP_011213804.1 1594261..1595985(+) (pilB) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGACAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATATCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTGCCCATGCTGGATATTAATTGCATCGATGTGGGCGCTATT
CCTGTCAATCTCGTTAATGAGAAATTAATTAAACGCCACGCCATGGTACCTCTTTTTAGTCGCGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAAGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCGATAGTTG
TGGAAGCAGATAAGCTTAGTGCTCTGATTGAAAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTGGAAGGTTTGGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCCCCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATACCGAATCAGGTACAGGCAAGATGGTATTTTACATGAGGTCGCTACCCCCCCGGCAAGCTTGTCA
TCTCGTATTACAGCACGCATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAGGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGTACATGTCCAACTTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGCGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATCATG
GTTGGTGAGATACGTGATCTTGAAACAGCAGAGATTGCTATAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAACATGGGAATACCTACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGTGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAGCTGATTTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTTGGGTTGTTTGAGGTTTTACCGATGACGAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGCTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6VPZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

99.477

100

0.995
  pilB Acinetobacter baylyi ADP1

49.828

100

0.505
  pilB Acinetobacter baumannii D1279779

50.794

98.78

0.502
  pilB Vibrio cholerae strain A1552

50.741

94.077

0.477
  pilB Vibrio campbellii strain DS40M4

47.756

97.038

0.463
  pilF Neisseria gonorrhoeae MS11

46.996

98.606

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.98

93.902

0.46
  pilF Thermus thermophilus HB27

39.147

93.902

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

92.16

0.366