Detailed information NTG0484972

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IF130_RS07230
• Genome accession: NZ_CP061840
• Coordinates: 1597257..1598120 (+)
• Length: 287 a.a.
• NCBI ID: WP_011213806.1
• Uniprot ID: I7HZ94
• Organism: Legionella pneumophila subsp. pneumophila strain AW-13-4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1592257..1603120

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IF130_RS07210 (IF130_07210)	-	1592750..1593076 (-)	327	WP_011213802.1	DUF1820 family protein	-
IF130_RS07215 (IF130_07215)	-	1593259..1594002 (-)	744	WP_011213803.1	methyltransferase domain-containing protein	-
IF130_RS07220 (IF130_07220)	pilB	1594261..1595985 (+)	1725	WP_011213804.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IF130_RS07225 (IF130_07225)	pilC	1595991..1597211 (+)	1221	WP_011213805.1	type II secretion system F family protein	Machinery gene
IF130_RS07230 (IF130_07230)	pilD	1597257..1598120 (+)	864	WP_011213806.1	A24 family peptidase	Machinery gene
IF130_RS07235 (IF130_07235)	-	1598134..1599339 (-)	1206	WP_011213807.1	cyclic nucleotide-binding domain-containing protein	-
IF130_RS07240 (IF130_07240)	-	1599447..1600040 (-)	594	WP_011213808.1	hypothetical protein	-
IF130_RS07245 (IF130_07245)	-	1600056..1600511 (-)	456	WP_011213809.1	hypothetical protein	-
IF130_RS07250 (IF130_07250)	-	1600645..1601460 (+)	816	WP_011213810.1	pyruvate, water dikinase regulatory protein	-
IF130_RS07255 (IF130_07255)	-	1601580..1603028 (-)	1449	WP_011213811.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32817.40 Da
• Isoelectric Point: 7.8442

>NTDB_id=484972 IF130_RS07230 WP_011213806.1 1597257..1598120(+) (pilD) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYIFLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=484972 IF130_RS07230 WP_011213806.1 1597257..1598120(+) (pilD) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCTGTTGGAAGTTT
GCTTAATGTGATAATCTACCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTTTTAAGAGGACGCTGTTACCAATGCGATAGTCCAATTTCAATTCGCTA
CCCTTTTGTTGAGACTCTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAACTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATATATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCTTTATGGCTATTCATTAATCTGTTTTATCTGATGACAGGTAAGGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACGGGGGCT
ATTATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: I7HZ94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	48.352	95.122	0.46
pilD	Acinetobacter baumannii D1279779	47.636	95.819	0.456
pilD	Vibrio campbellii strain DS40M4	45.324	96.864	0.439
pilD	Neisseria gonorrhoeae MS11	42.446	96.864	0.411