Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IF130_RS05020 Genome accession   NZ_CP061840
Coordinates   1085032..1085631 (+) Length   199 a.a.
NCBI ID   WP_011213355.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. pneumophila strain AW-13-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1080032..1090631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF130_RS05005 (IF130_05005) - 1081978..1082985 (-) 1008 WP_011213352.1 hypothetical protein -
  IF130_RS05010 (IF130_05010) pilM 1083402..1084466 (+) 1065 WP_011213353.1 type IV pilus assembly protein PilM Machinery gene
  IF130_RS05015 (IF130_05015) pilN 1084470..1085018 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  IF130_RS05020 (IF130_05020) pilO 1085032..1085631 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  IF130_RS05025 (IF130_05025) pilP 1085628..1086212 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  IF130_RS05030 (IF130_05030) pilQ 1086216..1088312 (+) 2097 WP_011213357.1 type IV pilus secretin PilQ Machinery gene
  IF130_RS05035 (IF130_05035) aroK 1088758..1089285 (+) 528 WP_010946667.1 shikimate kinase AroK -
  IF130_RS05040 (IF130_05040) aroB 1089272..1090381 (+) 1110 WP_011213358.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22523.24 Da        Isoelectric Point: 5.3369

>NTDB_id=484964 IF130_RS05020 WP_011213355.1 1085032..1085631(+) (pilO) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=484964 IF130_RS05020 WP_011213355.1 1085032..1085631(+) (pilO) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
ATGGACACGGTCAATTTAAATGAATTAACTCTAGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTAATTTT
AGGCATCAGTATACTGATAATTGGATTAGGATATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGCTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCTGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATCGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACCTTGCATGAATTTAGTGTTGAGGGGGTATCATCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGCCATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

100

100

1
  comO Acinetobacter baylyi ADP1

39.791

95.98

0.382
  pilO Acinetobacter baumannii D1279779

38.22

95.98

0.367