Detailed information NTG0484962

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IF130_RS05010
• Genome accession: NZ_CP061840
• Coordinates: 1083402..1084466 (+)
• Length: 354 a.a.
• NCBI ID: WP_011213353.1
• Uniprot ID: A0A3A6VL78
• Organism: Legionella pneumophila subsp. pneumophila strain AW-13-4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1078402..1089466

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IF130_RS05000 (IF130_05000)	-	1079444..1081828 (-)	2385	WP_011213351.1	penicillin-binding protein 1A	-
IF130_RS05005 (IF130_05005)	-	1081978..1082985 (-)	1008	WP_011213352.1	hypothetical protein	-
IF130_RS05010 (IF130_05010)	pilM	1083402..1084466 (+)	1065	WP_011213353.1	type IV pilus assembly protein PilM	Machinery gene
IF130_RS05015 (IF130_05015)	pilN	1084470..1085018 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
IF130_RS05020 (IF130_05020)	pilO	1085032..1085631 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
IF130_RS05025 (IF130_05025)	pilP	1085628..1086212 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
IF130_RS05030 (IF130_05030)	pilQ	1086216..1088312 (+)	2097	WP_011213357.1	type IV pilus secretin PilQ	Machinery gene
IF130_RS05035 (IF130_05035)	aroK	1088758..1089285 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38788.79 Da
• Isoelectric Point: 5.0367

>NTDB_id=484962 IF130_RS05010 WP_011213353.1 1083402..1084466(+) (pilM) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKRLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=484962 IF130_RS05010 WP_011213353.1 1083402..1084466(+) (pilM) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAGATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A3A6VL78

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.655	100	0.427
pilM	Acinetobacter baumannii D1279779	42.373	100	0.424
comM	Acinetobacter baylyi ADP1	40.96	100	0.41