Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IEE84_RS00315 Genome accession   NZ_CP061739
Coordinates   70005..71720 (-) Length   571 a.a.
NCBI ID   WP_191114491.1    Uniprot ID   -
Organism   Psychrobacter sp. 28M-43     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 65005..76720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEE84_RS00290 (IEE84_00290) - 65549..65884 (+) 336 WP_191114488.1 pyrimidine/purine nucleoside phosphorylase -
  IEE84_RS00295 (IEE84_00295) rlmB 66108..66878 (+) 771 WP_102078746.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IEE84_RS00300 (IEE84_00300) coaE 66841..67527 (-) 687 WP_191114489.1 dephospho-CoA kinase -
  IEE84_RS00305 (IEE84_00305) pilD 67654..68553 (-) 900 WP_191115319.1 prepilin peptidase Machinery gene
  IEE84_RS00310 (IEE84_00310) pilC 68561..69784 (-) 1224 WP_191114490.1 type II secretion system F family protein Machinery gene
  IEE84_RS00315 (IEE84_00315) pilB 70005..71720 (-) 1716 WP_191114491.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IEE84_RS00320 (IEE84_00320) tpiA 72105..72917 (+) 813 WP_191114492.1 triose-phosphate isomerase -
  IEE84_RS00325 (IEE84_00325) secG 73132..73440 (+) 309 WP_191114493.1 preprotein translocase subunit SecG -
  IEE84_RS00340 (IEE84_00340) rimP 74318..74818 (+) 501 WP_191114494.1 ribosome maturation factor RimP -
  IEE84_RS00345 (IEE84_00345) nusA 74954..76438 (+) 1485 WP_057757751.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63053.31 Da        Isoelectric Point: 4.9343

>NTDB_id=484422 IEE84_RS00315 WP_191114491.1 70005..71720(-) (pilB) [Psychrobacter sp. 28M-43]
MSIATSKYGGLAQQLISDGVVSEANMKTAQTESQQQQIGLVPYLVDNKLADAYQLAQMLSQAFGDPLFDLDALNIDVIPK
DLVDEKIVRKFNALPLFKRGQRLFVALSDPTRVDAIDAIAFNSRLSIETIVVEENKLKKRIESAYADTMQSFDSFSDSDL
NVGFEDGDEDKEDGETQLNDGVDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSFRVRFRVDGVMQKMANPPVQLANKIA
ARLKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQQEMFLEALHK
PQGMLLITGPTGSGKTVSLYTGINILNTGATNISTAEDPVEINLEGINQVNVNAKVGLTFANALKSFLRQDPDIVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLEIG
FTDTDLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVSPDISRIIMEDGNAIDIKDAAIKNGFRDLRRSGILKVLQGVT
SIQEMMRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=484422 IEE84_RS00315 WP_191114491.1 70005..71720(-) (pilB) [Psychrobacter sp. 28M-43]
ATGTCTATCGCAACCAGTAAGTATGGTGGTCTAGCGCAGCAACTTATTAGCGACGGTGTCGTTAGCGAAGCGAATATGAA
GACGGCACAGACTGAGTCGCAACAGCAGCAAATAGGACTGGTACCTTATCTGGTAGACAATAAATTGGCCGATGCTTATC
AACTTGCCCAAATGCTATCCCAAGCTTTCGGCGACCCTCTATTTGATCTTGATGCATTAAACATTGATGTTATTCCAAAA
GACTTGGTGGATGAAAAAATCGTTCGCAAGTTTAATGCATTACCACTCTTTAAACGAGGACAACGACTGTTTGTCGCGCT
GAGTGATCCCACACGCGTTGATGCTATTGATGCAATAGCCTTTAATTCTCGACTGTCGATAGAAACCATTGTTGTCGAAG
AAAATAAGCTCAAAAAGCGTATAGAAAGTGCATATGCAGATACGATGCAAAGCTTTGATAGCTTCTCTGATAGTGATTTG
AATGTTGGCTTTGAAGACGGTGATGAAGATAAAGAGGATGGCGAAACTCAGCTGAACGACGGCGTCGATGAAGCACCTGT
CGTAAAATTCGTCAATAAAATGCTAGTCGATGCTATCCGTATGGGTGCTTCTGATTTACATTTCGAGCCATACGAAAAAT
CTTTCAGAGTACGATTTCGTGTCGATGGCGTCATGCAAAAAATGGCCAATCCACCGGTTCAACTTGCGAATAAAATTGCA
GCACGCTTAAAGGTCATGTCTCAGATGGATATCTCAGAACGTCGGGTACCACAAGATGGGCGTATTAAACTAAAAATTTC
TAAAAATAAAGCGATTGATTTCCGGGTAAACTCATTACCGACGTTATTCGGTGAAAAGCTTGTTTTGCGTATCCTTGACC
CATCATCAGCCATGCTAGGTATTGATGCATTGGGCTATGAACCAGATCAGCAAGAAATGTTTTTAGAAGCATTACACAAA
CCACAAGGCATGTTGCTTATCACTGGCCCTACTGGTTCTGGTAAGACTGTCTCCCTTTACACTGGTATCAATATCTTGAA
TACTGGCGCAACCAATATCTCAACAGCTGAAGATCCAGTCGAAATTAACCTTGAAGGGATTAACCAAGTCAATGTCAATG
CCAAAGTTGGTCTGACGTTTGCCAATGCGCTCAAGTCTTTTTTACGTCAAGATCCTGATATTGTCATGGTCGGTGAGATT
CGTGACCTTGAGACTGCTGAGATTGCTATTAAAGCGGCGCAGACAGGTCATATGGTTCTTTCTACCTTGCATACCAACTC
GGCACCTGAAACATTAACGCGTCTACGCAATATGGGCGTCGCCTCCTTCAACATTGCCACATCAGTCAACCTAGTTATTG
CCCAACGATTGGCACGACGTCTCTGCAAGAACTGTAAAAAGCCTATTAGCATTCCGCGTCAGAGCTTATTAGAGATTGGT
TTTACCGATACTGATTTAGATAACACTGATAATATCATTTATGAACCAGTAGGCTGTAACGAATGCCGTGAAGGTTATAA
AGGTCGTGTAGGTATTTATGAAGTCATGAAAGTGAGCCCTGATATTTCACGAATTATTATGGAAGACGGAAATGCAATAG
ATATCAAAGACGCTGCCATTAAAAATGGTTTCCGTGATTTGCGTCGTTCTGGGATTTTAAAAGTGTTGCAAGGTGTCACA
AGTATTCAAGAAATGATGCGAGTAACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.717

99.65

0.685
  pilB Acinetobacter baylyi ADP1

64.841

99.124

0.643
  pilB Legionella pneumophila strain ERS1305867

50.697

100

0.51
  pilF Neisseria gonorrhoeae MS11

50.089

97.898

0.49
  pilB Vibrio campbellii strain DS40M4

48.029

97.723

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

49.813

93.87

0.468
  pilB Vibrio cholerae strain A1552

49.529

92.995

0.461