Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IEE84_RS00305 Genome accession   NZ_CP061739
Coordinates   67654..68553 (-) Length   299 a.a.
NCBI ID   WP_191115319.1    Uniprot ID   -
Organism   Psychrobacter sp. 28M-43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 62654..73553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEE84_RS00285 (IEE84_00285) recN 63105..64784 (-) 1680 WP_191114487.1 DNA repair protein RecN -
  IEE84_RS00290 (IEE84_00290) - 65549..65884 (+) 336 WP_191114488.1 pyrimidine/purine nucleoside phosphorylase -
  IEE84_RS00295 (IEE84_00295) rlmB 66108..66878 (+) 771 WP_102078746.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IEE84_RS00300 (IEE84_00300) coaE 66841..67527 (-) 687 WP_191114489.1 dephospho-CoA kinase -
  IEE84_RS00305 (IEE84_00305) pilD 67654..68553 (-) 900 WP_191115319.1 prepilin peptidase Machinery gene
  IEE84_RS00310 (IEE84_00310) pilC 68561..69784 (-) 1224 WP_191114490.1 type II secretion system F family protein Machinery gene
  IEE84_RS00315 (IEE84_00315) pilB 70005..71720 (-) 1716 WP_191114491.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IEE84_RS00320 (IEE84_00320) tpiA 72105..72917 (+) 813 WP_191114492.1 triose-phosphate isomerase -
  IEE84_RS00325 (IEE84_00325) secG 73132..73440 (+) 309 WP_191114493.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 33057.64 Da        Isoelectric Point: 8.9270

>NTDB_id=484420 IEE84_RS00305 WP_191115319.1 67654..68553(-) (pilD) [Psychrobacter sp. 28M-43]
MQFIQLLQENSTLALFVFGLLGLCVGSFLNVVIHRIPLMMVYGWRQECSQFMSEQADMPREHTLPIANIVATDQPITLSR
PASRCPHCAHKIKWYENIPLISWLLLRGRCSSCKAAIGLRYPLVELVTAILSVLVIYKFGVNATGLSAMVLVWTLVALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQSWFVTPAQSIWGLLLGFLSLWIVVKVFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGLILMRKQGESRPFAFGPYIAIAGIVALLYGSDIVSWYLGMYV

Nucleotide


Download         Length: 900 bp        

>NTDB_id=484420 IEE84_RS00305 WP_191115319.1 67654..68553(-) (pilD) [Psychrobacter sp. 28M-43]
ATGCAATTTATACAGTTACTACAAGAGAACAGTACCCTAGCTTTATTCGTATTTGGCCTATTAGGTCTTTGCGTTGGCAG
TTTTCTAAATGTTGTGATTCATCGCATACCGCTGATGATGGTCTATGGTTGGAGACAAGAGTGTAGCCAATTTATGTCTG
AACAGGCAGATATGCCACGCGAGCATACGCTACCTATAGCAAATATTGTCGCTACTGACCAACCAATTACTTTAAGTCGA
CCTGCCTCACGCTGTCCTCATTGCGCACATAAAATTAAGTGGTATGAAAATATCCCTTTAATAAGTTGGCTGTTATTACG
TGGACGCTGTTCAAGCTGCAAAGCGGCTATCGGTCTGCGTTATCCTCTCGTTGAGCTAGTAACTGCCATCCTATCCGTAT
TAGTAATATATAAGTTTGGGGTAAACGCTACAGGTTTGTCCGCAATGGTTTTAGTATGGACATTGGTAGCTCTAACAGGT
ATCGACTTTGACACACAGCTACTACCTGATCGTCTCACCTTTCCATTGGCAGGCTTAGGGTTGGCGGTGAATTCACAAAG
CTGGTTTGTCACACCAGCACAGTCGATATGGGGATTACTACTTGGGTTTTTATCGCTATGGATAGTAGTCAAGGTATTCT
ATTTAATTACTAAGAAACATGGAATGGGTCAAGGGGATTTTAAACTACTAGCAGTGTTAGGTGCTTGGCTTGGACCTATG
ATGTTACCTTTGATTATTCTACTATCATCTTTACTCGGCTCTATCGTTGGGCTAATCTTGATGAGAAAACAGGGTGAAAG
CCGACCTTTCGCTTTCGGACCTTATATCGCTATTGCAGGTATTGTTGCCTTATTATACGGCTCAGATATCGTCAGCTGGT
ATCTCGGTATGTATGTCTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(149-258)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

53.472

96.321

0.515
  pilD Acinetobacter baumannii D1279779

52.941

96.656

0.512
  pilD Vibrio cholerae strain A1552

48.239

94.983

0.458
  pilD Vibrio campbellii strain DS40M4

48.571

93.645

0.455
  pilD Neisseria gonorrhoeae MS11

40.972

96.321

0.395