Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IDM46_RS10775 Genome accession   NZ_CP061714
Coordinates   2301840..2303564 (+) Length   574 a.a.
NCBI ID   WP_185115699.1    Uniprot ID   A0A7X0QPE6
Organism   Luteimonas sp. MC1825     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2296840..2308564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IDM46_RS10755 (IDM46_10755) - 2297368..2298735 (-) 1368 WP_223877959.1 oligosaccharide flippase family protein -
  IDM46_RS10760 (IDM46_10760) - 2298651..2299682 (-) 1032 WP_185115696.1 glycosyltransferase -
  IDM46_RS10765 (IDM46_10765) - 2299761..2300864 (-) 1104 WP_185115697.1 UDP-N-acetylglucosamine 2-epimerase -
  IDM46_RS10770 (IDM46_10770) - 2300857..2301708 (-) 852 WP_185115698.1 glycosyltransferase family 2 protein -
  IDM46_RS10775 (IDM46_10775) pilB 2301840..2303564 (+) 1725 WP_185115699.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IDM46_RS10780 (IDM46_10780) pilC 2303855..2305009 (+) 1155 WP_223878074.1 type II secretion system F family protein Machinery gene
  IDM46_RS10785 (IDM46_10785) - 2305014..2305874 (+) 861 WP_185115701.1 A24 family peptidase -
  IDM46_RS10790 (IDM46_10790) coaE 2305876..2306493 (+) 618 WP_185115702.1 dephospho-CoA kinase -
  IDM46_RS10795 (IDM46_10795) - 2306499..2306765 (+) 267 WP_185115703.1 hypothetical protein -
  IDM46_RS10800 (IDM46_10800) - 2306777..2307247 (-) 471 WP_185115704.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62542.96 Da        Isoelectric Point: 5.5952

>NTDB_id=484348 IDM46_RS10775 WP_185115699.1 2301840..2303564(+) (pilB) [Luteimonas sp. MC1825]
MNAVSAANLVGITGIARRLVLDGVLDDAAARDALEKATQERKPIALYLREHKLVSSAQMAAANSIEFGMPVFDPLTMDGS
LSAIKLVSEELLSKHAVLPLFKRGSRLFVGISDPTNTPAMDEVKFHTNLAVEPILIDEDKIRRCVELWQDAGDTFGDTLG
DSEGLENLDISGGEDEGGDSAVEAKGDDTPVVKFINKVLIDAIKKGASDIHFEPYENDYRVRLRIDGILKQVAKMPVKLN
QRVTARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGVEKLGYEPEQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAVALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKQPQELPDNAL
LAEGFTADEIHQGFTIYDAVGCDECTGGYKGRLGVYQVLPMSEDIQTIVLQGGNAIQIAEVAKASGVRDLRQSALLKVKN
GLTSLAEINRITVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=484348 IDM46_RS10775 WP_185115699.1 2301840..2303564(+) (pilB) [Luteimonas sp. MC1825]
ATGAACGCAGTTTCGGCAGCCAACCTCGTCGGCATCACCGGTATCGCCCGCCGCCTCGTACTCGACGGCGTGCTCGACGA
CGCCGCCGCACGCGATGCGCTCGAGAAGGCCACCCAGGAGCGCAAGCCGATCGCGCTCTACCTGCGCGAGCACAAGTTGG
TCAGTTCGGCACAGATGGCGGCGGCTAATTCGATTGAGTTCGGCATGCCGGTGTTCGACCCCCTCACCATGGACGGGAGC
TTGAGCGCCATCAAGCTGGTGAGCGAGGAGCTTCTCTCCAAGCATGCGGTACTGCCGCTCTTCAAACGCGGCAGCCGGTT
GTTCGTCGGCATCTCAGACCCCACCAATACGCCGGCGATGGATGAGGTCAAGTTCCATACCAACCTCGCGGTCGAGCCAA
TCCTGATTGACGAGGACAAGATCCGTCGCTGCGTGGAGCTCTGGCAGGACGCTGGCGATACCTTCGGCGATACGCTGGGC
GACTCCGAGGGCCTGGAAAACCTTGACATCTCGGGAGGCGAGGATGAAGGGGGAGACTCAGCCGTCGAGGCCAAGGGCGA
CGACACCCCGGTCGTCAAGTTCATCAACAAGGTGCTGATCGACGCCATCAAGAAGGGCGCCTCGGACATCCACTTTGAGC
CGTATGAGAACGATTACCGGGTTCGCCTGCGCATCGACGGCATCCTGAAGCAGGTAGCCAAGATGCCCGTGAAGCTGAAC
CAGCGTGTCACTGCGCGTCTCAAGGTCATGGCCCAGCTCGATATCGCCGAGAAGCGGGTCCCTCAGGACGGTCGTATCAA
GCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAAAAGGTGGTGCTGC
GCATCCTGGACGGCAGCGCCGCCAAGCTTGGCGTGGAGAAGCTCGGCTACGAGCCCGAACAGCAGAAGCTGTTCCTGGAC
GCTATCCACAAGCCCTACGGCATGGTGCTGGTCACTGGCCCCACCGGCTCCGGCAAGACGGTGAGCCTGTACACCGCGCT
GGGCATCCTGAACGACGAGACCCGCAATATCTCGACCGCCGAGGACCCGGTGGAAATCCGCCTGCCGGGCGTGAACCAGG
TCCAGCAGAACGTAAAGCGCGGCATGACCTTTGCCGTAGCGCTCCGCAGCTTCCTCCGCCAGGACCCGGACATCATCATG
GTGGGCGAGATCCGCGATCTGGAGACGGCGGAGATCGCCATCAAGGCAGCACAAACCGGCCACATGGTGCTTTCCACCCT
GCACACGAACGACGCGCCCCAGACCATTGCCCGTCTGATGAACATGGGCATTGCCCCTTACAACATCACCAGTTCGGTGA
CCCTGGTGATCGCGCAGCGCCTGGCGAGACGCCTGTGCAACAAGTGCAAGCAGCCGCAGGAGTTGCCCGACAACGCGCTC
TTGGCCGAAGGCTTCACGGCCGACGAGATCCACCAGGGATTCACCATCTACGATGCGGTGGGCTGCGACGAGTGCACCGG
CGGCTACAAGGGCCGGCTTGGCGTGTACCAGGTGCTGCCGATGAGTGAGGATATCCAGACGATCGTCCTTCAAGGCGGGA
ATGCCATCCAGATCGCCGAGGTGGCCAAGGCCTCCGGCGTCAGGGATCTGCGCCAATCGGCGCTGCTGAAGGTGAAAAAC
GGGCTGACCAGCCTGGCGGAGATCAATCGCATCACCGTGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X0QPE6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.723

98.258

0.528
  pilB Acinetobacter baylyi ADP1

53.451

98.432

0.526
  pilB Legionella pneumophila strain ERS1305867

51.761

98.955

0.512
  pilF Neisseria gonorrhoeae MS11

48.063

98.955

0.476
  pilB Vibrio cholerae strain A1552

51.751

89.547

0.463
  pilB Vibrio campbellii strain DS40M4

51.12

85.54

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

50.202

86.411

0.434
  pilF Thermus thermophilus HB27

39.847

90.941

0.362