Detailed information NTG0483839

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC774_RS01595
• Genome accession: NZ_CP061655
• Coordinates: 343457..344515 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS33
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 338457..349515

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC774_RS01580 (IC774_01580)	-	339564..339896 (+)	333	WP_077168921.1	hypothetical protein	-
IC774_RS01585 (IC774_01585)	-	339899..340723 (+)	825	WP_143221083.1	putative RNA methyltransferase	-
IC774_RS01590 (IC774_01590)	ponA	340741..343295 (-)	2555	Protein_310	penicillin-binding protein PBP1a	-
IC774_RS01595 (IC774_01595)	pilM	343457..344515 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC774_RS01600 (IC774_01600)	pilN	344515..345159 (+)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC774_RS01605 (IC774_01605)	pilO	345156..345896 (+)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC774_RS01610 (IC774_01610)	pilP	345907..346434 (+)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC774_RS01615 (IC774_01615)	pilQ	346496..348622 (+)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC774_RS01620 (IC774_01620)	aroK	348634..349203 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483839 IC774_RS01595 WP_004705187.1 343457..344515(+) (pilM) [Acinetobacter seifertii strain AS33]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483839 IC774_RS01595 WP_004705187.1 343457..344515(+) (pilM) [Acinetobacter seifertii strain AS33]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426