Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   IC774_RS01600 Genome accession   NZ_CP061655
Coordinates   344515..345159 (+) Length   214 a.a.
NCBI ID   WP_004705190.1    Uniprot ID   N8R2F5
Organism   Acinetobacter seifertii strain AS33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339515..350159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC774_RS01580 (IC774_01580) - 339564..339896 (+) 333 WP_077168921.1 hypothetical protein -
  IC774_RS01585 (IC774_01585) - 339899..340723 (+) 825 WP_143221083.1 putative RNA methyltransferase -
  IC774_RS01590 (IC774_01590) ponA 340741..343295 (-) 2555 Protein_310 penicillin-binding protein PBP1a -
  IC774_RS01595 (IC774_01595) pilM 343457..344515 (+) 1059 WP_004705187.1 pilus assembly protein PilM Machinery gene
  IC774_RS01600 (IC774_01600) pilN 344515..345159 (+) 645 WP_004705190.1 PilN domain-containing protein Machinery gene
  IC774_RS01605 (IC774_01605) pilO 345156..345896 (+) 741 WP_077168923.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC774_RS01610 (IC774_01610) pilP 345907..346434 (+) 528 WP_077168924.1 pilus assembly protein PilP Machinery gene
  IC774_RS01615 (IC774_01615) pilQ 346496..348622 (+) 2127 WP_151792203.1 type IV pilus secretin PilQ family protein Machinery gene
  IC774_RS01620 (IC774_01620) aroK 348634..349203 (+) 570 WP_004705195.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24168.55 Da        Isoelectric Point: 5.3957

>NTDB_id=483840 IC774_RS01600 WP_004705190.1 344515..345159(+) (pilN) [Acinetobacter seifertii strain AS33]
MATINLLPWREELREQRKKQFITLCFGVVVLGITTVFGGWFYFDHKLDDQEQANQLITSTNQNLDQQLKALNGLQEQRDA
IIERMKLIQDLQSQRPVVVRLVDELVRVTPPAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
TEDKKDKAASSLLPRVEDHYGSFVVTVDLGEMGMTTADNNADKPAVAKDTGAAK

Nucleotide


Download         Length: 645 bp        

>NTDB_id=483840 IC774_RS01600 WP_004705190.1 344515..345159(+) (pilN) [Acinetobacter seifertii strain AS33]
ATGGCAACAATTAATTTACTACCTTGGCGTGAAGAGCTGAGGGAGCAAAGAAAAAAACAATTTATAACGTTATGCTTTGG
GGTGGTTGTGTTAGGAATTACAACCGTATTTGGGGGGTGGTTCTATTTTGATCATAAATTAGATGATCAAGAACAAGCAA
ATCAACTTATTACAAGTACTAATCAAAACCTTGATCAGCAATTAAAAGCATTAAATGGTTTACAAGAACAACGTGATGCC
ATCATTGAGCGTATGAAATTGATTCAAGATTTACAGAGTCAGCGTCCAGTCGTGGTAAGACTGGTTGATGAGTTAGTCAG
AGTCACGCCGCCTGCAATGTATTTAACGAAATTTAGCCGAACTGGGGATAAATTTACGATTGAAGGTAAAGCTGAAAGTC
CAAATACGGTAGCTGAACTACTCCGTAATCTAGAAGCATCTCCTTGGTATCGAAATGCTTTTATGAACTCTTTCTTGGCT
ACTGAAGATAAAAAAGATAAGGCAGCAAGTTCTCTTCTTCCTAGGGTAGAAGATCATTACGGTAGTTTTGTTGTGACTGT
CGATTTAGGGGAAATGGGCATGACCACAGCTGATAATAATGCTGATAAACCAGCCGTAGCTAAAGATACGGGGGCAGCAA
AATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8R2F5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

92.056

100

0.921
  comN Acinetobacter baylyi ADP1

70.616

98.598

0.696