Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IC774_RS01605 Genome accession   NZ_CP061655
Coordinates   345156..345896 (+) Length   246 a.a.
NCBI ID   WP_077168923.1    Uniprot ID   A0A7H2VHI1
Organism   Acinetobacter seifertii strain AS33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 340156..350896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC774_RS01590 (IC774_01590) ponA 340741..343295 (-) 2555 Protein_310 penicillin-binding protein PBP1a -
  IC774_RS01595 (IC774_01595) pilM 343457..344515 (+) 1059 WP_004705187.1 pilus assembly protein PilM Machinery gene
  IC774_RS01600 (IC774_01600) pilN 344515..345159 (+) 645 WP_004705190.1 PilN domain-containing protein Machinery gene
  IC774_RS01605 (IC774_01605) pilO 345156..345896 (+) 741 WP_077168923.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC774_RS01610 (IC774_01610) pilP 345907..346434 (+) 528 WP_077168924.1 pilus assembly protein PilP Machinery gene
  IC774_RS01615 (IC774_01615) pilQ 346496..348622 (+) 2127 WP_151792203.1 type IV pilus secretin PilQ family protein Machinery gene
  IC774_RS01620 (IC774_01620) aroK 348634..349203 (+) 570 WP_004705195.1 shikimate kinase AroK -
  IC774_RS01625 (IC774_01625) aroB 349232..350314 (+) 1083 WP_077168926.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27889.51 Da        Isoelectric Point: 4.8393

>NTDB_id=483841 IC774_RS01605 WP_077168923.1 345156..345896(+) (pilO) [Acinetobacter seifertii strain AS33]
MSQDELQELSLEQTSSKNKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLHAIDNAQAQE
SNLLNEFREKDSKLRNLRQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPTKDSKSDIPVLNYSIKAKTYRYVEATDTAEQSTQAPTPPASTS
TTAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=483841 IC774_RS01605 WP_077168923.1 345156..345896(+) (pilO) [Acinetobacter seifertii strain AS33]
ATGAGTCAAGATGAACTTCAAGAATTATCATTAGAGCAAACATCTTCAAAGAACAAGAAATTTAATCTCGATAAATTCCT
TCAACAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCCATATCTGTAAAAATTACTTGTTGGATTTTTATTT
TCTTTGCTGTATTAGCTTTAGGTTATTTTGTTGCTATTCAGCCAAAGCTACACGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAACTTGCGGCAGTATCAAGTTCAACTTCAAGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACAGAGATTCCTAGTTTGGTTGAGGATATTAACTTAA
CTGGTGTGAACTCAGGATTGAAATTTAAAAATATTCGCTTGGAAAATGAAGTCAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCCACAGGTGATTATCACGCCTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATCGTAAC
CATGCATGACTTTACTGTTGATGTATCTCCAACTAAAGACAGCAAGTCTGATATTCCTGTATTGAATTATTCTATTAAAG
CGAAAACCTATCGCTATGTAGAAGCAACAGATACAGCGGAGCAATCGACCCAAGCTCCAACACCACCTGCTTCAACTAGC
ACTACTGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2VHI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

91.463

100

0.915
  comO Acinetobacter baylyi ADP1

67.347

99.593

0.671