Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC793_RS17185 Genome accession   NZ_CP061571
Coordinates   3532315..3534027 (+) Length   570 a.a.
NCBI ID   WP_191102339.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS70     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3527315..3539027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC793_RS17150 (IC793_17145) nusA 3528204..3529688 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  IC793_RS17155 (IC793_17150) rimP 3529726..3530250 (-) 525 WP_191102338.1 ribosome maturation factor RimP -
  IC793_RS17175 (IC793_17170) secG 3530889..3531218 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  IC793_RS17180 (IC793_17175) tpiA 3531231..3532025 (-) 795 WP_151740589.1 triose-phosphate isomerase -
  IC793_RS17185 (IC793_17180) pilB 3532315..3534027 (+) 1713 WP_191102339.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC793_RS17190 (IC793_17185) - 3534055..3535280 (+) 1226 Protein_3395 type II secretion system F family protein -
  IC793_RS17195 (IC793_17190) pilD 3535280..3536140 (+) 861 WP_151740586.1 A24 family peptidase Machinery gene
  IC793_RS17200 (IC793_17195) coaE 3536142..3536738 (+) 597 WP_191012501.1 dephospho-CoA kinase -
  IC793_RS17205 (IC793_17200) - 3536735..3537649 (-) 915 WP_049064242.1 DMT family transporter -
  IC793_RS17210 (IC793_17205) rlmB 3537685..3538434 (-) 750 WP_039251214.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC793_RS17215 (IC793_17210) - 3538537..3538863 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63483.77 Da        Isoelectric Point: 5.1596

>NTDB_id=483295 IC793_RS17185 WP_191102339.1 3532315..3534027(+) (pilB) [Acinetobacter seifertii strain AS70]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEAHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFSDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECRDGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483295 IC793_RS17185 WP_191102339.1 3532315..3534027(+) (pilB) [Acinetobacter seifertii strain AS70]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGCGCATCTCTCTC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TTGAGCACCATAAACTCGAAAAAGTTTTAAACCAGCATTTCGCAGAAGAAAGCAGTTTTGATTTTAGTGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAGGATGAAGACGAAACACCTCAAGGTGATGAAGCACCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGGGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCCAATCCTCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGACCCAT
CAAGTGCGATGCTGGGGATTGACGCATTAGGATACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGCATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGTTTAAATATTTTAAATAC
TGAGAGCTCTAATATCTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCAA
AAGTAGGTCTCACTTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTTGGTGAGATTCGT
GATTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTGGAAATGGGCTTT
ACCGAACAGGACCTAGCACATCCAGACTTTCGGGTTTTTCAACCTGTGGGTTGTCCTGAATGTCGTGATGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGCGTGACTTCC
CTACAAGAAGTTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

96.667

100

0.967
  pilB Acinetobacter baylyi ADP1

79.474

100

0.795
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.057

99.298

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

51.331

92.281

0.474
  pilB Vibrio campbellii strain DS40M4

50.484

90.702

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381