Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSTAA_RS05155 Genome accession   NC_017532
Coordinates   1103902..1105605 (+) Length   567 a.a.
NCBI ID   WP_014595957.1    Uniprot ID   A0AA42HGA5
Organism   Stutzerimonas stutzeri DSM 4166     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1098902..1110605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSTAA_RS05135 (PSTAA_1013) cysD 1099027..1099944 (+) 918 WP_014595954.1 sulfate adenylyltransferase subunit CysD -
  PSTAA_RS05140 (PSTAA_1014) cysN 1099956..1101854 (+) 1899 WP_014595955.1 sulfate adenylyltransferase subunit CysN -
  PSTAA_RS21910 (PSTAA_1015) - 1102098..1102652 (-) 555 WP_080558856.1 REP-associated tyrosine transposase -
  PSTAA_RS21915 - 1103108..1103592 (-) 485 Protein_1030 pilin -
  PSTAA_RS05155 (PSTAA_1016) pilB 1103902..1105605 (+) 1704 WP_014595957.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSTAA_RS05160 (PSTAA_1017) pilC 1105608..1106825 (+) 1218 WP_014595958.1 type II secretion system F family protein Machinery gene
  PSTAA_RS05165 (PSTAA_1018) pilD 1106828..1107697 (+) 870 WP_014595959.1 prepilin peptidase Machinery gene
  PSTAA_RS05170 (PSTAA_1019) coaE 1107829..1108437 (+) 609 WP_014595960.1 dephospho-CoA kinase -
  PSTAA_RS05175 yacG 1108434..1108631 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  PSTAA_RS05180 (PSTAA_1020) - 1108644..1109324 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  PSTAA_RS05185 (PSTAA_1021) - 1109382..1110422 (-) 1041 WP_014595961.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62067.14 Da        Isoelectric Point: 5.6480

>NTDB_id=48283 PSTAA_RS05155 WP_014595957.1 1103902..1105605(+) (pilB) [Stutzerimonas stutzeri DSM 4166]
MSENISLSGLARQMVVAGVLDEKTAQQAQAQAVRGKTPFVTYVVQNKLAKGRAIAELASDQFGVALLDLSALNKESLPKD
LISEKLARQHRVLPLSKRGTKLYVAISDPANHQSIADIQFSTGLSIESILVEDDKLGEAIDKLYESGATGLEELGEVDLD
GVDVESVTEDGKAEEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVDVPRDVLLREGFP
EQKLGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=48283 PSTAA_RS05155 WP_014595957.1 1103902..1105605(+) (pilB) [Stutzerimonas stutzeri DSM 4166]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGTTGGACGAAAAAACGGCCCAGCA
GGCGCAGGCTCAAGCCGTTCGCGGCAAAACACCCTTTGTCACCTATGTAGTGCAGAACAAGTTGGCCAAAGGCCGCGCCA
TAGCCGAGCTTGCTTCCGATCAATTCGGTGTAGCGCTGCTGGACCTGTCTGCGCTGAACAAGGAGTCGCTGCCCAAGGAT
CTGATCAGTGAGAAGTTGGCCCGCCAACATCGGGTGCTGCCGCTTTCCAAGCGCGGCACCAAGCTTTATGTCGCCATCTC
CGATCCGGCTAATCATCAATCGATTGCCGATATCCAGTTCAGTACCGGGCTGAGCATCGAGTCGATTCTGGTGGAAGACG
ACAAGCTGGGCGAGGCCATCGACAAACTGTACGAAAGCGGCGCCACCGGACTAGAGGAGCTGGGCGAAGTCGATCTGGAT
GGGGTCGATGTCGAGTCCGTCACCGAAGATGGAAAAGCTGAGGAAGGCGGAGAGGCTGCTGACGACGCCCCTGTAGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGTGGTGGCTCGTCCGATTTGCACTTCGAGCCCTACGAAAAAGCCTACC
GCGTGCGGCTTCGCACGGATGGCATCCTGCATGAGGTCGCGCGTCCGCCGATTCAGCTTGCACCGCGAATTTCTGCGCGC
CTAAAGGTCATGGCCGGGCTGGATATCTCCGAGCGGCGTAAGCCGCAGGATGGCCGGATCAAGATGAAGCTGTCTAAGAC
CAAGGCCATCGATTTCCGCGTAAATACGCTACCCACCCTATGGGGCGAGAAAATCGTGATGCGTATCCTCGACCCGTCCA
GTGCGCAGATGGGGATCGATGCCCTGGGTTACGAGGAAGAACAGAAAGAGCTCTACATGAATGCCTTGCGCCAGCCACAA
GGCATGATCCTGGTTACCGGCCCGACTGGGTCGGGCAAGACGGTTTCGCTGTATACCGGTCTGAATATCCTCAATACCGT
GGACGTGAACATCTCCACCGCGGAAGACCCAGTGGAGATCAATCTGGAGGGTATTAACCAGGTCAACGTAAACCCGCGCC
AAGGTATGGACTTCGCTCAGGCCCTCCGTGCCTTTCTACGTCAGGACCCGGACATCATCATGGTAGGGGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCTGCGCAGACCGGGCATATGGTGATGTCGACCCTGCACACCAACAGCGCCGC
CGAAACGCTTACCCGTCTGCGCAATATGGGTGTGCCCGCGTTCAATATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCACGAAAGCTCTGCAATAGCTGCAAGAAAGAGGTTGATGTCCCCCGTGACGTTTTGCTGCGCGAAGGGTTCCCT
GAGCAGAAGCTTGGAACCTTCAAGATTTATGGTCCGGTAGGTTGCGACAACTGCAAGGGTGGCTACAAGGGGCGCGTCGG
TATTTATGAAGTGGTTAAAAACACGCCATCGTTGCAGCGGATTATCATGGAAGACGGAAACTCCATCGATATTTCAACCC
AGATGCGCAAAGACGGCTTCAACGACCTGCGAACCTCGGCTTTGCTGAAAGCCATGCAGGGCGTGACCAGCCTCGAAGAA
GTCAATCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.106

99.471

0.598
  pilB Acinetobacter baylyi ADP1

59.503

99.295

0.591
  pilB Legionella pneumophila strain ERS1305867

54.912

100

0.552
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.492
  pilF Neisseria gonorrhoeae MS11

48.845

99.295

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

52.692

91.711

0.483
  pilB Vibrio cholerae strain A1552

52.827

90.476

0.478
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.633

96.12

0.381

Multiple sequence alignment