Detailed information NTG0481846

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D8K17_RS08905
• Genome accession: NZ_CP061322
• Coordinates: 1754027..1754674 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis strain S50
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1749027..1759674

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D8K17_RS08880 (D8K17_008900)	atpF	1749097..1749603 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
D8K17_RS08885 (D8K17_008905)	atpB	1749618..1750331 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
D8K17_RS08890 (D8K17_008910)	-	1750376..1750591 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
D8K17_RS08895 (D8K17_008915)	-	1750775..1751551 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
D8K17_RS08900 (D8K17_008920)	comEC	1751836..1754046 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D8K17_RS08905 (D8K17_008925)	comEA	1754027..1754674 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
D8K17_RS08910 (D8K17_008930)	-	1754734..1756095 (-)	1362	WP_021214597.1	ABC transporter permease	-
D8K17_RS08915 (D8K17_008935)	-	1756092..1757024 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
D8K17_RS08920 (D8K17_008940)	-	1757129..1757527 (-)	399	WP_004255236.1	hypothetical protein	-
D8K17_RS08925 (D8K17_008945)	-	1757631..1758194 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
D8K17_RS08930 (D8K17_008950)	-	1758367..1759545 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=481846 D8K17_RS08905 WP_010906131.1 1754027..1754674(-) (comEA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=481846 D8K17_RS08905 WP_010906131.1 1754027..1754674(-) (comEA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386