Detailed information NTG0481084

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: IAU66_RS12935
• Genome accession: NZ_CP061168
• Coordinates: 2672292..2672906 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain T-5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2667292..2677906

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IAU66_RS12905	rpsT	2667592..2667858 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
IAU66_RS12910	holA	2667875..2668916 (-)	1042	Protein_2525	DNA polymerase III subunit delta	-
IAU66_RS12915	-	2668956..2669110 (-)	155	Protein_2526	hypothetical protein	-
IAU66_RS12920	-	2669151..2669285 (+)	135	WP_003152870.1	YqzM family protein	-
IAU66_RS12925	comEC	2669304..2671655 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IAU66_RS12930	-	2671656..2672225 (-)	570	WP_003152868.1	ComE operon protein 2	-
IAU66_RS12935	comEA	2672292..2672906 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
IAU66_RS12940	comER	2672965..2673786 (+)	822	WP_014305449.1	late competence protein ComER	-
IAU66_RS12945	-	2673855..2674592 (-)	738	WP_024085626.1	class I SAM-dependent methyltransferase	-
IAU66_RS12950	rsfS	2674589..2674945 (-)	357	WP_003152864.1	ribosome silencing factor	-
IAU66_RS12955	yqeK	2674963..2675523 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IAU66_RS12960	-	2675513..2676082 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
IAU66_RS12965	yhbY	2676093..2676383 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
IAU66_RS12970	aroE	2676377..2677219 (-)	843	WP_188322870.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=481084 IAU66_RS12935 WP_041482377.1 2672292..2672906(-) (comEA) [Bacillus amyloliquefaciens strain T-5]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=481084 IAU66_RS12935 WP_041482377.1 2672292..2672906(-) (comEA) [Bacillus amyloliquefaciens strain T-5]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363