Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IAE60_RS18370 Genome accession   NZ_CP060731
Coordinates   3913127..3914863 (+) Length   578 a.a.
NCBI ID   WP_187573332.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain GTZY2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3908127..3919863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAE60_RS18355 (IAE60_18355) pilA2 3908582..3908998 (+) 417 WP_187573329.1 pilin Machinery gene
  IAE60_RS18360 (IAE60_18360) - 3909063..3911030 (+) 1968 WP_187573330.1 hypothetical protein -
  IAE60_RS18365 (IAE60_18365) - 3911027..3912976 (+) 1950 WP_187573331.1 hypothetical protein -
  IAE60_RS18370 (IAE60_18370) pilB 3913127..3914863 (+) 1737 WP_187573332.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IAE60_RS18375 (IAE60_18375) pilC 3915087..3916343 (+) 1257 WP_187573333.1 type II secretion system F family protein Machinery gene
  IAE60_RS18380 (IAE60_18380) pilD 3916353..3917216 (+) 864 WP_187573334.1 A24 family peptidase Machinery gene
  IAE60_RS18385 (IAE60_18385) coaE 3917250..3917867 (+) 618 WP_187573335.1 dephospho-CoA kinase -
  IAE60_RS18390 (IAE60_18390) - 3918014..3918439 (-) 426 WP_244966612.1 transposase -
  IAE60_RS18395 (IAE60_18395) - 3918818..3919768 (-) 951 WP_187573337.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62575.93 Da        Isoelectric Point: 5.9527

>NTDB_id=478154 IAE60_RS18370 WP_187573332.1 3913127..3914863(+) (pilB) [Pseudoxanthomonas mexicana strain GTZY2]
MNAVATPNLVGITGIARRLVQDGAMDEATARSAMANASEAKVPLAQWVAEKRLVAPAHLAAANAIEFGMPLIDVTAFDPA
HHAVKLVSEELIQKHQVLPLFKRGGKLFVGTSNPTQTAALTEIQFHTNLVVEPVLVDDDQLRRTLDQWLNAASNLSGSFG
GDDDEGLDNLEVGTDEDMGGGADSAVDAKGDDTPVVKFVNKVLVDAIKRGASDIHFEPYETDYRVRLRIDGILKQVAKAP
VKLSSRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPDQQK
LFEEAIQKPYGMVLVTGPTGSGKTVSLYTALGILNDEVRNISTVEDPVEIRLPGVNQVQQNTKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPKCKKPVELP
KPALLAEGFTEAEIDAGFTLYDAVGCDECTEGYKGRTGIYQVMPMSDDIQAIVLEGGNAMQIAAVAQKAGIRDLRQSALM
KVRNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=478154 IAE60_RS18370 WP_187573332.1 3913127..3914863(+) (pilB) [Pseudoxanthomonas mexicana strain GTZY2]
ATGAACGCTGTCGCCACACCCAATCTGGTGGGCATCACCGGCATCGCCCGCCGCCTGGTCCAGGACGGCGCGATGGATGA
AGCCACCGCGCGCAGCGCGATGGCCAACGCTTCCGAGGCCAAGGTGCCATTGGCGCAGTGGGTGGCGGAGAAGCGGCTGG
TGGCCCCGGCCCACCTGGCCGCCGCCAACGCCATCGAATTCGGCATGCCGCTGATCGATGTCACTGCGTTCGACCCCGCC
CATCACGCCGTCAAGCTGGTCAGCGAGGAGCTGATCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTCGGCACCAGCAATCCCACCCAGACCGCGGCCCTGACCGAAATCCAGTTCCACACCAACCTGGTGGTGGAGCCCG
TGCTGGTCGATGATGACCAGCTGCGTCGCACCCTGGACCAGTGGCTCAACGCCGCCAGCAACCTGTCCGGCAGCTTCGGC
GGCGACGACGACGAGGGCCTGGACAACCTGGAGGTGGGTACCGACGAGGACATGGGCGGTGGCGCGGACAGCGCCGTCGA
CGCCAAAGGCGACGACACGCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTGGACGCCATCAAGCGCGGCGCTTCGGACA
TCCACTTCGAACCCTACGAGACCGACTACCGCGTACGCCTGCGCATCGACGGCATCCTCAAGCAGGTGGCCAAGGCGCCG
GTGAAGCTGAGCTCGCGCATCGCCGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAAAAGCGCGTGCCGCAGGA
CGGCCGCATCAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACACTGCCCACGCTGTTCGGCGAGA
AGATCGTGCTGCGCATCCTGGACGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAG
CTTTTCGAAGAAGCCATCCAGAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGCT
GTACACGGCGCTGGGCATCCTGAACGACGAGGTCCGCAACATCTCCACTGTCGAGGACCCGGTGGAAATCCGCCTGCCCG
GCGTGAACCAGGTGCAGCAGAACACCAAACGCGGCATGACCTTCGCCGCGGCCCTGCGCAGCTTCCTGCGCCAAGACCCC
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACATGGT
GCTGTCCACCCTGCACACCAACGACGCCCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCCCCGTACAACATCA
CCAGCTCAGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGGCGGCTGTGCCCCAAGTGCAAGAAACCGGTCGAGCTGCCC
AAGCCCGCCCTGCTGGCCGAAGGCTTCACCGAGGCCGAGATCGACGCCGGCTTCACCCTGTACGACGCGGTCGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGGACCGGCATCTACCAGGTCATGCCGATGTCCGACGACATTCAGGCCATCGTGC
TGGAAGGCGGCAACGCCATGCAGATCGCCGCCGTCGCCCAGAAGGCCGGCATCCGCGACCTGCGCCAGTCCGCACTGATG
AAGGTCCGCAACGGCGTCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.667

98.616

0.559
  pilB Acinetobacter baylyi ADP1

54.922

100

0.55
  pilB Legionella pneumophila strain ERS1305867

52.113

98.27

0.512
  pilF Neisseria gonorrhoeae MS11

49.825

98.616

0.491
  pilB Vibrio cholerae strain A1552

52.317

89.619

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.384

98.097

0.455
  pilB Vibrio campbellii strain DS40M4

45.439

98.616

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.409

100

0.375