Detailed information NTG0477830

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: H9W85_RS24155
• Genome accession: NZ_CP060703
• Coordinates: 5230365..5232065 (+)
• Length: 566 a.a.
• NCBI ID: WP_187682590.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NRD619
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5212897..5229605	5230365..5232065	flank	760

Gene organization within MGE regions

Location: 5212897..5232065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9W85_RS24075 (H9W85_24075)	-	5212897..5213202 (+)	306	WP_034010508.1	hypothetical protein	-
H9W85_RS29935	-	5213205..5213642 (+)	438	WP_309039959.1	hypothetical protein	-
H9W85_RS24085 (H9W85_24085)	-	5213681..5214037 (+)	357	WP_262411292.1	hypothetical protein	-
H9W85_RS24090 (H9W85_24090)	-	5214037..5215199 (+)	1163	WP_086008654.1	IS3-like element IS222 family transposase	-
H9W85_RS24095 (H9W85_24095)	-	5216052..5216216 (+)	165	WP_153519866.1	hypothetical protein	-
H9W85_RS24100 (H9W85_24100)	-	5216233..5217825 (+)	1593	WP_023082018.1	anti-phage dCTP deaminase	-
H9W85_RS24105 (H9W85_24105)	-	5218628..5219134 (-)	507	WP_034064628.1	GNAT family N-acetyltransferase	-
H9W85_RS24110 (H9W85_24110)	-	5219908..5220177 (+)	270	WP_262411293.1	hypothetical protein	-
H9W85_RS24115 (H9W85_24115)	-	5220177..5220422 (+)	246	WP_187682587.1	hypothetical protein	-
H9W85_RS24120 (H9W85_24120)	-	5221197..5221583 (-)	387	WP_187682588.1	ankyrin repeat domain-containing protein	-
H9W85_RS24125 (H9W85_24125)	-	5221567..5224341 (-)	2775	WP_187682589.1	RHS repeat-associated core domain-containing protein	-
H9W85_RS24130 (H9W85_24130)	-	5224356..5225504 (-)	1149	WP_166737577.1	DUF6531 domain-containing protein	-
H9W85_RS24135 (H9W85_24135)	-	5227719..5227961 (+)	243	WP_023109353.1	hypothetical protein	-
H9W85_RS24145 (H9W85_24145)	-	5228220..5229578 (-)	1359	WP_238335499.1	O-antigen ligase family protein	-
H9W85_RS24150 (H9W85_24150)	-	5229670..5230134 (-)	465	Protein_4788	pilin	-
H9W85_RS24155 (H9W85_24155)	pilB	5230365..5232065 (+)	1701	WP_187682590.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62360.30 Da
• Isoelectric Point: 5.5785

>NTDB_id=477830 H9W85_RS24155 WP_187682590.1 5230365..5232065(+) (pilB) [Pseudomonas aeruginosa strain NRD619]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPYVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=477830 H9W85_RS24155 WP_187682590.1 5230365..5232065(+) (pilB) [Pseudomonas aeruginosa strain NRD619]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGC
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGTACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTCACCCGCCTGCTGAACATGGGTGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (190-457)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.368	100	0.578
pilB	Acinetobacter baylyi ADP1	56.614	100	0.567
pilB	Legionella pneumophila strain ERS1305867	54.321	100	0.544
pilB	Vibrio cholerae strain A1552	51.342	98.763	0.507
pilF	Neisseria gonorrhoeae MS11	50	99.647	0.498
pilB	Vibrio parahaemolyticus RIMD 2210633	51.97	94.17	0.489
pilB	Vibrio campbellii strain DS40M4	51.032	94.17	0.481
pilB/pilB1	Synechocystis sp. PCC 6803	37.318	100	0.408
pilF	Thermus thermophilus HB27	38.489	100	0.387
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.545	97.173	0.375