Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J1G50_RS03085 Genome accession   NZ_CP071489
Coordinates   566198..567583 (-) Length   461 a.a.
NCBI ID   WP_160405120.1    Uniprot ID   -
Organism   Glaesserella parasuis strain YHP1815     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561198..572583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G50_RS03060 (J1G50_03060) clpP 561215..561796 (+) 582 WP_005711766.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  J1G50_RS03065 (J1G50_03065) clpX 561803..563053 (+) 1251 WP_160405112.1 ATP-dependent protease ATP-binding subunit ClpX Regulator
  J1G50_RS03070 (J1G50_03070) dapE 563127..564260 (-) 1134 WP_160405114.1 succinyl-diaminopimelate desuccinylase -
  J1G50_RS03075 (J1G50_03075) - 564344..565012 (-) 669 WP_160405116.1 prepilin peptidase -
  J1G50_RS03080 (J1G50_03080) pilC 565009..566205 (-) 1197 WP_160405118.1 type II secretion system F family protein Machinery gene
  J1G50_RS03085 (J1G50_03085) pilB 566198..567583 (-) 1386 WP_160405120.1 GspE/PulE family protein Machinery gene
  J1G50_RS03090 (J1G50_03090) pilA 567630..568094 (-) 465 WP_160405122.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J1G50_RS03095 (J1G50_03095) radA/sms 568282..569658 (+) 1377 WP_005711784.1 DNA repair protein RadA Machinery gene
  J1G50_RS03100 (J1G50_03100) gmk 569711..570337 (-) 627 WP_071610761.1 guanylate kinase -
  J1G50_RS03105 (J1G50_03105) gpt 570575..571054 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52626.34 Da        Isoelectric Point: 4.9724

>NTDB_id=477205 J1G50_RS03085 WP_160405120.1 566198..567583(-) (pilB) [Glaesserella parasuis strain YHP1815]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPLYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLTQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCCGTGC
DQCYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=477205 J1G50_RS03085 WP_160405120.1 566198..567583(-) (pilB) [Glaesserella parasuis strain YHP1815]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGACATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAACCTGTGGTAATTGCTTCTGATGAG
CTTAAATACCTGCTTAACGCACTCTCGCCTGAGCAACAACCCCTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAACCCAAAATGCGT
CTGATATTCATATTGAACCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATCAGCGGTCGAACGTTTGTTACAACTAGGTATTCAAGAGTATG
AAATCCAAAATTCGCTATTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTTGTGGCACAGGCTGT
GACCAATGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGCTTAAGCAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGACTTTTCCAGCTTACGAGAGAGTGCCAAACAGAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

96.529

100

0.965
  pilB Haemophilus influenzae 86-028NP

56.957

99.783

0.568
  pilB Haemophilus influenzae Rd KW20

57.582

98.698

0.568
  pilB Vibrio cholerae strain A1552

38.554

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.293

100

0.41
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Acinetobacter baumannii D1279779

35.798

100

0.399
  pilB Legionella pneumophila strain ERS1305867

40.909

95.445

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.085

100

0.388
  pilF Neisseria gonorrhoeae MS11

36.632

100

0.377