Detailed information NTG0476750

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: H9L11_RS03675
• Genome accession: NZ_CP060589
• Coordinates: 800967..802652 (+)
• Length: 561 a.a.
• NCBI ID: WP_033905593.1
• Uniprot ID: A0AAW9B5F0
• Organism: Vibrio sp. sp1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	791213..808892	800967..802652	within	0

Gene organization within MGE regions

Location: 791213..808892

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9L11_RS03640 (H9L11_03640)	lpdA	791213..792640 (-)	1428	WP_005379993.1	dihydrolipoyl dehydrogenase	-
H9L11_RS03645 (H9L11_03645)	aceF	792908..794800 (-)	1893	WP_033905589.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
H9L11_RS03650 (H9L11_03650)	aceE	794820..797483 (-)	2664	WP_005386612.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
H9L11_RS03655 (H9L11_03655)	pdhR	797532..798299 (-)	768	WP_005379989.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
H9L11_RS03660 (H9L11_03660)	ampD	798705..799256 (-)	552	WP_005386614.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
H9L11_RS03665 (H9L11_03665)	nadC	799349..800236 (+)	888	WP_033905590.1	carboxylating nicotinate-nucleotide diphosphorylase	-
H9L11_RS03670 (H9L11_03670)	pilA	800499..800963 (+)	465	WP_033905592.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
H9L11_RS03675 (H9L11_03675)	pilB	800967..802652 (+)	1686	WP_033905593.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H9L11_RS03680 (H9L11_03680)	pilC	802676..803899 (+)	1224	WP_205390996.1	type II secretion system F family protein	Machinery gene
H9L11_RS03685 (H9L11_03685)	pilD	803964..804833 (+)	870	WP_017635919.1	A24 family peptidase	Machinery gene
H9L11_RS03690 (H9L11_03690)	coaE	804834..805448 (+)	615	WP_017635920.1	dephospho-CoA kinase	-
H9L11_RS03695 (H9L11_03695)	zapD	805476..806216 (+)	741	WP_005373086.1	cell division protein ZapD	-
H9L11_RS03700 (H9L11_03700)	yacG	806375..806569 (+)	195	WP_017635922.1	DNA gyrase inhibitor YacG	-
H9L11_RS03705 (H9L11_03705)	rplS	806882..807235 (-)	354	WP_005379971.1	50S ribosomal protein L19	-
H9L11_RS03710 (H9L11_03710)	trmD	807277..808038 (-)	762	WP_033905595.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
H9L11_RS03715 (H9L11_03715)	rimM	808066..808614 (-)	549	WP_005379964.1	ribosome maturation factor RimM	-
H9L11_RS03720 (H9L11_03720)	rpsP	808644..808892 (-)	249	WP_005379962.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 561 a.a.
• Molecular weight: 62103.87 Da
• Isoelectric Point: 5.4031

>NTDB_id=476750 H9L11_RS03675 WP_033905593.1 800967..802652(+) (pilB) [Vibrio sp. sp1]
MQTNLSTILRQKGLLAFSQEESLLEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAVADPTNQQAEDDFRFATGLQVELVLGEFSQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide

• Length: 1686 bp

>NTDB_id=476750 H9L11_RS03675 WP_033905593.1 800967..802652(+) (pilB) [Vibrio sp. sp1]
ATGCAAACCAACCTCTCCACGATTCTTCGTCAAAAAGGACTATTGGCTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACATCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTATGCTTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACGCGGCATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTACTCGCCGTTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCCACTGGTTTACAGGTTGAATTGGTCTTGGGGGAATTTAGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTATTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TGCGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTGAGTCGTCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTGCCTACTCTATTTGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGTCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACCGGGCTGAGTATCCTCAATAAACCAGAAATCAA
CATTTCTACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCGGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAGATCGCCATCAAAGCCTCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAACTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGAATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (188-455)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (38-144)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio parahaemolyticus RIMD 2210633	95.187	100	0.952
pilB	Vibrio campbellii strain DS40M4	89.661	100	0.897
pilB	Vibrio cholerae strain A1552	74.021	100	0.742
pilB	Legionella pneumophila strain ERS1305867	48.746	99.465	0.485
pilB	Acinetobacter baumannii D1279779	51.81	93.583	0.485
pilB	Acinetobacter baylyi ADP1	47.935	99.287	0.476
pilF	Neisseria gonorrhoeae MS11	45.035	100	0.453
pilB/pilB1	Synechocystis sp. PCC 6803	37.268	100	0.394
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	36.684	100	0.371
pilF	Thermus thermophilus HB27	36.299	100	0.364