Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H7A79_RS09885 Genome accession   NZ_CP060414
Coordinates   1872636..1874315 (-) Length   559 a.a.
NCBI ID   WP_135034423.1    Uniprot ID   -
Organism   Neisseria musculi strain NW831     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1867636..1879315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7A79_RS09860 - 1868684..1868881 (-) 198 WP_187000128.1 DNA gyrase inhibitor YacG -
  H7A79_RS09865 (H7A79_1818) zapD 1868891..1869649 (-) 759 WP_135034419.1 cell division protein ZapD -
  H7A79_RS09870 (H7A79_1819) coaE 1869646..1870266 (-) 621 WP_187000129.1 dephospho-CoA kinase -
  H7A79_RS09875 (H7A79_1820) pilD 1870377..1871240 (-) 864 WP_187000130.1 A24 family peptidase Machinery gene
  H7A79_RS09880 (H7A79_1821) pilG 1871242..1872480 (-) 1239 WP_135034422.1 type II secretion system F family protein Machinery gene
  H7A79_RS09885 (H7A79_1822) pilF 1872636..1874315 (-) 1680 WP_135034423.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7A79_RS09895 (H7A79_1824) - 1875012..1875632 (+) 621 WP_135034424.1 isochorismatase family protein -
  H7A79_RS09900 - 1875932..1876057 (-) 126 WP_255518792.1 hypothetical protein -
  H7A79_RS09905 (H7A79_1825) groES 1876211..1876498 (+) 288 WP_135034425.1 co-chaperone GroES -
  H7A79_RS09910 (H7A79_1826) groL 1876577..1878217 (+) 1641 WP_187000131.1 chaperonin GroEL -
  H7A79_RS09915 (H7A79_1827) - 1878582..1879232 (-) 651 WP_187000132.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62143.79 Da        Isoelectric Point: 6.5915

>NTDB_id=475841 H7A79_RS09885 WP_135034423.1 1872636..1874315(-) (pilF) [Neisseria musculi strain NW831]
MSVGILRVLVQSQIVDAAQVERYQSVLKSNKSILPLLFEEGVITPKALGELLARVFSYPLLDLRYYPRSNILSDVLSEEQ
MLQNRCIPIFQRGRKVYFAVSDPTRIQSFQKIAFASGISVDLVVVCDDQLNTLLEWLGQRSTSILNEMSKEHDASTPAQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGALNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCQACKAPMERPPVSALKKAGFTDEDLAKDWVM
YRAVGCDSCRGKGYKGRAGVYEVMPITEEMQRVIMNNGTEVDIMNMAYKEGMVDLRRSGLLKVMQGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=475841 H7A79_RS09885 WP_135034423.1 1872636..1874315(-) (pilF) [Neisseria musculi strain NW831]
ATGAGCGTCGGTATATTAAGAGTATTGGTGCAAAGCCAAATCGTTGATGCAGCACAAGTCGAACGGTATCAAAGCGTATT
GAAAAGCAATAAGTCCATCCTTCCTCTGTTGTTTGAAGAAGGTGTGATTACGCCCAAAGCGTTGGGGGAGTTGCTGGCGA
GGGTGTTTAGTTATCCGTTGTTGGATCTGCGTTATTATCCGCGCAGCAATATCCTTTCAGATGTGCTTTCAGAAGAGCAG
ATGCTGCAAAACCGCTGTATTCCGATTTTTCAGCGGGGGAGAAAAGTTTATTTCGCGGTGTCTGATCCTACCCGAATTCA
GAGTTTTCAAAAAATTGCTTTCGCTTCCGGTATATCGGTTGATTTAGTTGTGGTGTGCGATGATCAACTGAACACCCTGC
TTGAGTGGCTGGGTCAGCGCTCGACTTCTATCTTGAACGAAATGAGCAAGGAGCATGACGCCTCCACTCCTGCCCAGGCG
CTGTATATCGATAATGAAGAGGCGGAAGATGGGCCGATTCCCCGCTTTATCCATAAAACACTTTCAGATGCATTGAATGC
CGGTGCGTCCGATATCCATTTCGAGTTTTACGAACAGATGGCGAGGGTGCGTTTCCGCGTGGACGGCCAGCTGCGCGAAG
TGGTGCAGCCGCCTGTGGCTGTGCGCGGCCAGTTGGCATCGCGTATCAAAGTGATGGCACGTTTGGATATTTCAGAAAAA
CGTGTGCCGCAAGACGGCCGTATTCAAATTGCTTTTCACAAGCACGGCCGCCCGATTGACTTCCGTGTCAGTACGCTGCC
TACCCTGTTTGGCGAAAAAGTAGTGATGCGTATTTTGAATTCGGATGCGGGGGCGCTCAATATCGATCAGCTCGGTTTCG
AGCCTTTTCAAAAAGAGATGCTGCTTGAAGCCATTCACCGCCCATATGGAATGGTGCTGGTAACAGGCCCTACCGGATCA
GGTAAAACAGTGTCGCTTTATACCTGCTTGAATATTTTGAATACCGAGAGTGTGAATATTGCCACTGCGGAAGACCCGGC
CGAGATTAATCTGCCGGGCATCAACCAAGTAAACGTAAATGATAAACAGGGACTTACTTTTGCGGCAGCGCTGAAGTCTT
TCCTGCGCCAAGACCCGGATATCATTATGGTGGGCGAGATTCGTGACTTGGAAACAGCGGATATTGCCATTAAGGCCGCA
CAAACCGGGCATATGGTGTTTTCTACTCTGCATACCAACAATGCACCGGCCACTTTGTCGCGTATGTTGAATATGGGGGT
GGCTCCTTTCAATATTGCCAGTTCGGTCAGCCTGATCATGGCGCAACGCTTATTGCGCCGCTTATGCCAGGCCTGCAAGG
CGCCGATGGAACGCCCGCCTGTATCGGCTTTGAAAAAAGCGGGTTTTACAGACGAAGATTTGGCTAAAGACTGGGTAATG
TATCGTGCAGTAGGGTGTGATAGCTGCCGTGGAAAAGGCTATAAAGGCCGTGCCGGTGTTTATGAGGTAATGCCGATTAC
CGAAGAAATGCAGCGGGTGATTATGAATAACGGCACCGAAGTGGATATTATGAATATGGCTTATAAAGAAGGTATGGTGG
ATTTGCGCCGTTCCGGTTTGTTAAAAGTAATGCAGGGGATAACTTCTTTAGAAGAAGTTTTAGCTCACACCAACGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

78.533

100

0.785
  pilB Acinetobacter baumannii D1279779

52.603

99.642

0.524
  pilB Acinetobacter baylyi ADP1

51.246

100

0.515
  pilB Legionella pneumophila strain ERS1305867

47.415

100

0.476
  pilB Vibrio cholerae strain A1552

46.099

100

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.456
  pilB Vibrio campbellii strain DS40M4

44.345

99.642

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.815

93.56

0.363