Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7F35_RS05085 Genome accession   NZ_CP060295
Coordinates   1070189..1071922 (+) Length   577 a.a.
NCBI ID   WP_187111867.1    Uniprot ID   -
Organism   Variovorax sp. PAMC26660     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1065189..1076922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F35_RS05050 (H7F35_05050) - 1065223..1065786 (+) 564 WP_261803529.1 CNP1-like family protein -
  H7F35_RS05055 (H7F35_05055) proB 1065824..1066966 (-) 1143 WP_187111864.1 glutamate 5-kinase -
  H7F35_RS05060 (H7F35_05060) cgtA 1067043..1068119 (-) 1077 WP_187111865.1 Obg family GTPase CgtA -
  H7F35_RS05065 (H7F35_05065) rpmA 1068191..1068451 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  H7F35_RS05070 (H7F35_05070) rplU 1068464..1068775 (-) 312 WP_187111866.1 50S ribosomal protein L21 -
  H7F35_RS05075 (H7F35_05075) - 1068957..1069949 (+) 993 WP_187114149.1 polyprenyl synthetase family protein -
  H7F35_RS05085 (H7F35_05085) pilB 1070189..1071922 (+) 1734 WP_187111867.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7F35_RS05090 (H7F35_05090) pilC 1071947..1073176 (+) 1230 WP_187111868.1 type II secretion system F family protein Machinery gene
  H7F35_RS05095 (H7F35_05095) - 1073176..1074141 (+) 966 WP_187111869.1 prepilin peptidase -
  H7F35_RS05100 (H7F35_05100) coaE 1074150..1074758 (+) 609 WP_187111870.1 dephospho-CoA kinase -
  H7F35_RS05105 (H7F35_05105) - 1074832..1076325 (+) 1494 WP_187111871.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62694.91 Da        Isoelectric Point: 5.7436

>NTDB_id=475515 H7F35_RS05085 WP_187111867.1 1070189..1071922(+) (pilB) [Variovorax sp. PAMC26660]
MAAAELPVKEPTQIALPGLARALVSAGKLPAKIAEDIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
NSIVGSEFEFDDVSADTSGDANEQAIAEVEDAPVVRFLHKMLLDAVGMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKER
LLRAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCRTPVDVPR
EALLDAGFREEDLDGSWKPYRPVGCSACSGGYKGRVGIYQVMPITEAIQEIILRDGSALDIAKQSETEGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=475515 H7F35_RS05085 WP_187111867.1 1070189..1071922(+) (pilB) [Variovorax sp. PAMC26660]
ATGGCCGCTGCCGAACTTCCTGTTAAAGAACCTACGCAGATTGCCCTCCCCGGGCTTGCGCGCGCCCTGGTTTCGGCCGG
CAAACTCCCCGCCAAGATCGCAGAAGACATCTACCAGAAGTCGCTGAGCGGCCGCACCAGCTTCATTGCCGAGCTGACTG
GCACTGGCGCCGTGTCGGCCGCCGATCTGGCTCACACACTTTCAAGCGCCTTCGGTGCCCCGCTGCTCGACCTGGATGCC
ATTGATCACCAGCGCCTGCCCAAGGACCTGCTCGATCCGAAGCTTTGTCATGCGTACCGCATCGTCGTTCTCAGCAAACG
CAACAACCGCCTGATCGTTGCAACGGCGGATCCGTCTGATCAACAAGCGGCGGAGAAGATCAAGTTCGCTTCCCAGATGG
GTGTGGACTGGGTCATCGCCGAGTACGACAAGCTCTCCCGAATGATCGAAGCCGCCGCAGTCAGCGCGGCGGAGACCATC
AACAGCATCGTCGGCAGCGAGTTTGAATTCGACGATGTGTCCGCCGACACCTCGGGCGACGCGAACGAGCAGGCCATTGC
CGAGGTCGAGGACGCGCCGGTCGTTCGCTTCCTCCACAAGATGCTGCTCGATGCCGTCGGCATGCGGGCTTCGGACATCC
ACTTCGAGCCCTACGAGCACCACTACCGCGTGCGCTTTCGCGTCGACGGCGAGCTGCGTGAGATCGCCAGCCCGCCAACA
GTCATCAAGGACAAGCTGGCTTCGCGGATCAAGGTCATTTCCCGGCTCGACATCTCCGAAAAGCGCGTGCCGCAAGACGG
CCGCATGAAGCTCAAGATCGGGCCCGATCGCGTGATCGACTTTCGCGTGAGCACCTTGCCCACGCTGTTCGGCGAGAAGA
TCGTCGTTCGTATTCTTGACCCGAGCAGTGCACGCCTGGGCATCGATGCCCTCGGCTACGACGCGGACGAGAAAGAGCGC
CTTCTTCGCGCCATCGGCCGCCCCTACGGCATGGTGCTCGTGACCGGGCCCACCGGTTCCGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAATCTGCTCAACCAGCCGGGCGTCAACATCGCGACGGCGGAAGACCCTTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAGCGGGCGGGGCTGACCTTTGCCACCGCGCTGCGCGCCTTCCTGCGGCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACCGGTCACCTGGTGCT
GTCGACACTGCACACGAACGACGCGCCGACCACACTCACGCGGATGCGCAACATGGGCATTGCGCCCTTCAACATTGCGT
CCAGCGTGATCCTGATCACCGCGCAGCGCCTGGCGCGCAGACTGTGCACCGTCTGCCGCACGCCCGTGGATGTCCCGCGC
GAAGCGCTGCTCGACGCGGGCTTCAGGGAGGAAGACCTGGACGGCTCCTGGAAACCGTATCGGCCCGTCGGCTGCTCCGC
ATGCAGCGGCGGCTACAAAGGCCGGGTGGGCATCTACCAGGTGATGCCGATCACCGAAGCCATCCAGGAAATCATCCTGC
GCGATGGCAGCGCACTCGACATCGCAAAGCAGTCCGAGACCGAAGGCGTGCGTTCCCTGCGCCAGTCGGGTCTTCGAAAA
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTGGTGGCGTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.049

98.44

0.532
  pilB Acinetobacter baumannii D1279779

54.011

97.227

0.525
  pilF Neisseria gonorrhoeae MS11

52.575

97.574

0.513
  pilB Legionella pneumophila strain ERS1305867

48.929

97.054

0.475
  pilB Vibrio cholerae strain A1552

47.445

94.974

0.451
  pilB Vibrio campbellii strain DS40M4

45.633

97.227

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

45.617

96.88

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.147

94.107

0.378
  pilF Thermus thermophilus HB27

37.828

98.96

0.374