Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I5154_RS23495 Genome accession   NZ_CP060242
Coordinates   4999027..5000727 (+) Length   566 a.a.
NCBI ID   WP_034075677.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain B-I-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4994027..5005727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5154_RS23475 (I5154_004826) - 4994740..4996995 (-) 2256 WP_003094643.1 DUF1631 domain-containing protein -
  I5154_RS23480 (I5154_004827) nadC 4997281..4998129 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I5154_RS23490 (I5154_004829) - 4998333..4998797 (-) 465 WP_031630343.1 pilin -
  I5154_RS23495 (I5154_004830) pilB 4999027..5000727 (+) 1701 WP_034075677.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I5154_RS23500 (I5154_004831) pilC 5000731..5001951 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  I5154_RS23505 (I5154_004832) pilD 5001955..5002827 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  I5154_RS23510 (I5154_004833) coaE 5002824..5003435 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  I5154_RS23515 (I5154_004834) yacG 5003432..5003632 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  I5154_RS23520 (I5154_004835) - 5003669..5003878 (-) 210 WP_003094660.1 hypothetical protein -
  I5154_RS23525 (I5154_004836) - 5003984..5004673 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  I5154_RS23530 (I5154_004837) - 5004670..5005140 (-) 471 WP_003094664.1 hypothetical protein -
  I5154_RS23535 (I5154_004838) - 5005137..5005562 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62414.30 Da        Isoelectric Point: 5.5814

>NTDB_id=474669 I5154_RS23495 WP_034075677.1 4999027..5000727(+) (pilB) [Pseudomonas aeruginosa strain B-I-1]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
AISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQN
MILVTGPTGSGKTVSLYTGLNILNTTDINISTVEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=474669 I5154_RS23495 WP_034075677.1 4999027..5000727(+) (pilB) [Pseudomonas aeruginosa strain B-I-1]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GCCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAGCGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCGACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGACGCTCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTGGGCAAGGCAGAAAGCGCCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCAAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGAAC
ATGATTCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGTCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.368

100

0.578
  pilB Acinetobacter baylyi ADP1

56.261

100

0.564
  pilB Legionella pneumophila strain ERS1305867

54.291

100

0.548
  pilB Vibrio cholerae strain A1552

51.083

97.88

0.5
  pilF Neisseria gonorrhoeae MS11

49.823

99.647

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

52.247

94.346

0.493
  pilB Vibrio campbellii strain DS40M4

51.032

94.17

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.439

100

0.408
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378