Detailed information NTG0474186

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: H6X75_RS10075
• Genome accession: NZ_CP060156
• Coordinates: 1990533..1990994 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250530.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain PZ900700608
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1985533..1995994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H6X75_RS10045 (H6X75_10035)	-	1985838..1986662 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
H6X75_RS10050 (H6X75_10040)	rnpA	1986637..1987008 (-)	372	WP_000739246.1	ribonuclease P protein component	-
H6X75_RS11300	-	1987025..1987156 (-)	132	WP_000768904.1	hypothetical protein	-
H6X75_RS10055 (H6X75_10045)	-	1987157..1988347 (-)	1191	WP_000167766.1	acetate kinase	-
H6X75_RS10060 (H6X75_10050)	comYH	1988398..1989351 (-)	954	WP_000345117.1	class I SAM-dependent methyltransferase	Machinery gene
H6X75_RS10065 (H6X75_10055)	-	1989412..1990005 (-)	594	Protein_1971	methyltransferase domain-containing protein	-
H6X75_RS10070 (H6X75_10060)	comGG/cglG	1990142..1990555 (-)	414	WP_000265630.1	competence type IV pilus minor pilin ComGG	Machinery gene
H6X75_RS10075 (H6X75_10065)	comGF/cglF	1990533..1990994 (-)	462	WP_000250530.1	competence type IV pilus minor pilin ComGF	Machinery gene
H6X75_RS10080 (H6X75_10070)	comGE/cglE	1990957..1991259 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
H6X75_RS10085 (H6X75_10075)	comGD/cglD	1991222..1991626 (-)	405	WP_000588006.1	competence type IV pilus minor pilin ComGD	Machinery gene
H6X75_RS10090 (H6X75_10080)	comGC/cglC	1991619..1991945 (-)	327	WP_000738638.1	comG operon protein ComGC	Machinery gene
H6X75_RS10095 (H6X75_10085)	comGB/cglB	1991947..1992963 (-)	1017	WP_075270915.1	competence type IV pilus assembly protein ComGB	Machinery gene
H6X75_RS10100 (H6X75_10090)	comGA/cglA/cilD	1992911..1993852 (-)	942	WP_000249556.1	competence type IV pilus ATPase ComGA	Machinery gene
H6X75_RS10105 (H6X75_10095)	-	1993927..1994292 (-)	366	WP_000286415.1	DUF1033 family protein	-
H6X75_RS10110 (H6X75_10100)	-	1994443..1995501 (-)	1059	WP_000649471.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17883.39 Da
• Isoelectric Point: 6.4832

>NTDB_id=474186 H6X75_RS10075 WP_000250530.1 1990533..1990994(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700608]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=474186 H6X75_RS10075 WP_000250530.1 1990533..1990994(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700608]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae D39	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae R6	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae TIGR4	94.771	100	0.948
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915