Detailed information NTG0472311

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: H4K32_RS15275
• Genome accession: NZ_CP059855
• Coordinates: 2953798..2954412 (+)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Pm9
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2948798..2959412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H4K32_RS15240	aroE	2949485..2950327 (+)	843	WP_025852995.1	shikimate dehydrogenase	-
H4K32_RS15245	yhbY	2950321..2950611 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
H4K32_RS15250	-	2950622..2951191 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
H4K32_RS15255	yqeK	2951181..2951762 (+)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
H4K32_RS15260	rsfS	2951759..2952115 (+)	357	WP_003152864.1	ribosome silencing factor	-
H4K32_RS15265	-	2952112..2952849 (+)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
H4K32_RS15270	comER	2952918..2953739 (-)	822	WP_014305449.1	late competence protein ComER	-
H4K32_RS15275	comEA	2953798..2954412 (+)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
H4K32_RS15280	-	2954479..2955048 (+)	570	WP_003152868.1	ComE operon protein 2	-
H4K32_RS15285	comEC	2955049..2957400 (+)	2352	WP_046559887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
H4K32_RS15290	-	2957419..2957553 (-)	135	WP_003152870.1	YqzM family protein	-
H4K32_RS15295	-	2957594..2957748 (+)	155	Protein_2985	hypothetical protein	-
H4K32_RS15300	holA	2957788..2958829 (+)	1042	Protein_2986	DNA polymerase III subunit delta	-
H4K32_RS15305	rpsT	2958846..2959112 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=472311 H4K32_RS15275 WP_003152867.1 2953798..2954412(+) (comEA) [Bacillus velezensis strain Pm9]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=472311 H4K32_RS15275 WP_003152867.1 2953798..2954412(+) (comEA) [Bacillus velezensis strain Pm9]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363