Detailed information NTG0472185

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H2O77_RS00880
• Genome accession: NZ_CP059843
• Coordinates: 190737..191891 (+)
• Length: 384 a.a.
• NCBI ID: WP_043333447.1
• Uniprot ID: A0AAP4TZ60
• Organism: Cobetia sp. 4B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 185737..196891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2O77_RS00860 (H2O77_00860)	-	186956..187537 (-)	582	WP_043333437.1	YggT family protein	-
H2O77_RS00865 (H2O77_00865)	proC	187687..188529 (-)	843	WP_215823712.1	pyrroline-5-carboxylate reductase	-
H2O77_RS00870 (H2O77_00870)	-	188618..189370 (-)	753	WP_215823713.1	YggS family pyridoxal phosphate-dependent enzyme	-
H2O77_RS00875 (H2O77_00875)	pilT	189566..190597 (+)	1032	WP_043333445.1	type IV pilus twitching motility protein PilT	Machinery gene
H2O77_RS00880 (H2O77_00880)	pilU	190737..191891 (+)	1155	WP_043333447.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H2O77_RS00885 (H2O77_00885)	-	191998..193146 (-)	1149	WP_215823714.1	anhydro-N-acetylmuramic acid kinase	-
H2O77_RS00890 (H2O77_00890)	tyrS	193302..194510 (+)	1209	WP_215823715.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43054.35 Da
• Isoelectric Point: 6.3429

>NTDB_id=472185 H2O77_RS00880 WP_043333447.1 190737..191891(+) (pilU) [Cobetia sp. 4B]
MTPHEWLHELLQLMVSKGSSDLFISTGTPPQMKVNGRMVALGDKKLSVDQVRELVLAPMSDMQRERFEEEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKGLSETKRGLVIFVGGTGAGKSTSLAAMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGNTTQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQGLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVLNAASRFSLQGDDD

Nucleotide

• Length: 1155 bp

>NTDB_id=472185 H2O77_RS00880 WP_043333447.1 190737..191891(+) (pilU) [Cobetia sp. 4B]
ATGACTCCTCATGAATGGCTGCATGAACTTCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGACCTGTTCATCTCCACCGG
CACACCGCCGCAGATGAAGGTCAATGGCCGCATGGTGGCGCTGGGTGACAAGAAGCTGAGTGTCGATCAGGTGAGAGAGC
TGGTGCTCGCACCGATGAGTGACATGCAGCGCGAGCGCTTCGAGGAAGAGCGCGAGGCCAACTTCGCGCATAGCTTGCCG
GGAGTCGGGCGCTTCAGGATCAGCGCCTTCTACCAGCGTAGCCAGATGGGCATGGTGATTCGCCGCATCCAGCTGTCGAT
TCCCAGCCTGGAAGAGTTGCGGCTGCCGGAGATCATCAAGGGGCTTTCCGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GAGGCACCGGCGCCGGCAAGTCGACCTCGCTGGCGGCGATGATCCAGCACCGCAACCAGACCTCGAGCGGGCACATCATC
TGCATCGAAGACCCGATCGAGTATATCCACCCCCACCAGCGCTCCATCGTGACGCAGCGTGAAGTGGGGATCGATACCGA
GTCCTTCGAGGTGGCACTGCGCAATACGCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCTCGCGTGAGA
CCATGGAGCACGCGCTGACCTTCGCCGAGACCGGTCACCTGTGTCTTGCGACGTTGCATGCCAACAATGCCAACCAGGCG
CTGGACCGCATCATCCACTTCTTCCCGGAGGAACGTCATGAACAGGTGTGGATGGATCTGTCGCTGAACCTCAAGGGCAT
CGTGGCCCAGCAGCTGTTGCCTCACAAGAGTGGCAACACCACCCAGCGCGTGCCGGCCATTGAGGTGATGCTGCGTTCGC
CGCTGATCGTCGACCTGATCCGCAAGGGCGCGGTGGTCGAGATCAAGGACGTCATGAAACGCTCGCAGCAGCAGGGCATG
ATGACCTTCGATCAATCGCTGTACGCCTTGCATCAGCAGGGCTTGATCACCGAGGAGGTGGCTTTGGCGCATGCCGATTC
CGCCAATGACCTGCGCCTGATGATCAAGTTCGGTGATTCCGACAGCGCCCAGGAGGCCCAGCTGGATGTCCTGAATGCCG
CCAGTCGCTTCTCGCTGCAAGGGGATGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (84-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.625	91.667	0.602
pilU	Vibrio cholerae strain A1552	56.64	96.094	0.544
pilU	Acinetobacter baylyi ADP1	55.932	92.188	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.348	89.844	0.398
pilT	Neisseria meningitidis 8013	40.816	89.323	0.365
pilT	Pseudomonas aeruginosa PAK	41.742	86.719	0.362