Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SG35_RS24830 Genome accession   NZ_CP059735
Coordinates   5695468..5697168 (+) Length   566 a.a.
NCBI ID   WP_053043441.1    Uniprot ID   A0AAF0C2F2
Organism   Thalassomonas actiniarum strain A5K-106     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5690468..5702168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SG35_RS24805 (SG35_024750) ampE 5691003..5691989 (-) 987 WP_044833611.1 beta-lactamase regulator AmpE -
  SG35_RS24810 (SG35_024755) ampD 5692036..5692623 (-) 588 WP_044833610.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SG35_RS24815 (SG35_024760) - 5692662..5693180 (+) 519 WP_044833609.1 retropepsin-like aspartic protease family protein -
  SG35_RS24820 (SG35_024765) nadC 5693184..5694074 (+) 891 WP_044833608.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SG35_RS24825 (SG35_024770) - 5694864..5695292 (+) 429 WP_044835944.1 pilin -
  SG35_RS24830 (SG35_024775) pilB 5695468..5697168 (+) 1701 WP_053043441.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SG35_RS24835 (SG35_024780) pilC 5697239..5698480 (+) 1242 WP_044835943.1 type II secretion system F family protein Machinery gene
  SG35_RS24840 (SG35_024785) pilD 5698532..5699416 (+) 885 WP_274055281.1 prepilin peptidase Machinery gene
  SG35_RS24845 (SG35_024790) coaE 5699492..5700103 (+) 612 WP_044835942.1 dephospho-CoA kinase -
  SG35_RS24850 (SG35_024795) zapD 5700335..5701084 (+) 750 WP_044835941.1 cell division protein ZapD -
  SG35_RS24855 (SG35_024800) yacG 5701175..5701411 (+) 237 WP_044835940.1 DNA gyrase inhibitor YacG -
  SG35_RS24860 (SG35_024805) mutT 5701445..5701843 (-) 399 WP_044835939.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62716.79 Da        Isoelectric Point: 5.0226

>NTDB_id=471668 SG35_RS24830 WP_053043441.1 5695468..5697168(+) (pilB) [Thalassomonas actiniarum strain A5K-106]
MKGHHQQSSLLSALSKHNLVPEELVDEISATFLQQKKPFIRYLVEDKNYNAVSIVSILSRSFGYPHIDLKNFDTSLVPEG
IRNEKLIHKHNALPLFLRGKVLFVAMSDPTNLDALEEIQFNTGFTTELVLTDENSLQTCIDKVLEEDSEALGITDIDAEE
LSDIDVQEERKEDDAGEDKDDAPIVVYINKILLDAIRKGASDLHFEPYEKSYRIRFRIDGILNEVAKPPVSLASRMAARL
KVMSKLDIAERRIPQDGRIKLALSKKKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIEMLGYEADQKEIYLEALARPQG
MILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPVEINLEGINQVQINNKAGLTFPSALRSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSIIIAQRLARRLCSQCKTEEMIPEEALLKQGFTP
EQIKDIKLYKAVGCEQCTGGYRGRVGIYEVIRISQETANIIMAGGNSLDIAAQCQTEGFNNLRQSGLVKAVQGVTSLEEV
NRVTSA

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=471668 SG35_RS24830 WP_053043441.1 5695468..5697168(+) (pilB) [Thalassomonas actiniarum strain A5K-106]
ATGAAAGGACACCATCAACAGTCAAGCTTACTCAGCGCCCTGTCCAAGCACAACTTAGTGCCCGAAGAACTGGTTGATGA
AATCAGCGCGACTTTTTTACAGCAAAAAAAGCCGTTTATCCGCTATTTAGTTGAAGATAAAAATTACAATGCCGTCAGCA
TTGTCAGTATCTTATCCCGCAGTTTTGGTTACCCCCATATAGACTTAAAGAACTTTGACACCTCTCTGGTACCCGAAGGT
ATCCGCAACGAAAAGCTGATCCACAAACATAATGCCCTGCCGCTTTTTTTACGCGGAAAAGTATTGTTTGTCGCCATGTC
AGATCCCACCAATTTAGATGCCCTGGAGGAAATACAATTTAATACCGGCTTTACAACCGAATTGGTGTTAACCGATGAAA
ACAGCCTGCAAACTTGTATAGACAAGGTACTGGAAGAAGACAGCGAAGCCCTGGGGATTACCGACATTGATGCGGAAGAA
CTCTCCGATATCGATGTCCAGGAAGAGCGCAAGGAAGACGATGCCGGTGAAGACAAGGACGATGCCCCTATAGTCGTTTA
TATCAATAAGATCCTATTGGATGCCATCAGAAAGGGGGCCTCTGATTTACACTTCGAACCCTATGAGAAGTCTTACCGCA
TTCGCTTTCGTATTGACGGCATTTTAAACGAAGTGGCTAAACCCCCGGTAAGCCTTGCGTCACGCATGGCGGCGCGGTTA
AAAGTCATGTCGAAACTCGATATTGCCGAGCGCCGCATCCCGCAGGATGGCCGTATCAAGCTCGCCTTATCCAAAAAGAA
ATCGATCGATTTTCGTGTCAGCACCCTGCCCACCATGTGGGGTGAAAAAATCGTAATGCGTATCCTCGACTCATCCAGCG
CCATGTTGGGTATAGAGATGTTAGGCTATGAAGCCGATCAAAAAGAAATCTACCTGGAAGCACTCGCCCGCCCGCAAGGC
ATGATTTTGGTCACCGGCCCCACCGGCTCGGGTAAAACCGTTTCTTTATACACGGGGCTAAATATCTTAAACACTCAGGA
GCGCAATATCTCCACCGCAGAAGATCCGGTTGAAATCAACCTCGAAGGCATCAACCAGGTCCAAATCAATAACAAGGCAG
GATTAACCTTCCCCAGCGCCCTGCGCTCTTTTTTAAGGCAAGATCCCGATATTGTCATGGTAGGTGAGATCCGGGATCTC
GAAACCGCAGAAATCGCCATTAAGGCTGCGCAAACCGGGCATTTAGTGCTGTCCACCCTGCATACCAACTCTGCTGCAGA
AACCCTCACCCGGCTACTGAATATGGGGGTACCTTCTTATAATGTTGCCAGCTCGGTCAGCATTATTATTGCCCAGCGCC
TGGCCCGACGCTTATGCAGCCAGTGTAAAACCGAAGAAATGATCCCGGAAGAAGCCCTGCTCAAGCAAGGCTTTACCCCG
GAGCAAATAAAAGATATTAAATTGTACAAGGCGGTCGGCTGCGAACAATGTACCGGGGGCTACCGGGGCAGGGTTGGCAT
TTACGAAGTTATCAGGATCAGCCAGGAAACGGCCAATATCATTATGGCAGGAGGTAACAGCCTGGATATCGCCGCCCAAT
GTCAGACTGAAGGATTTAATAACTTACGCCAATCAGGCCTGGTCAAGGCCGTGCAGGGCGTAACCAGTTTAGAAGAAGTT
AACCGGGTAACAAGCGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.74

95.23

0.569
  pilB Legionella pneumophila strain ERS1305867

57.374

98.233

0.564
  pilB Acinetobacter baylyi ADP1

59.439

94.523

0.562
  pilB Vibrio cholerae strain A1552

55.114

93.286

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

52.102

96.643

0.504
  pilF Neisseria gonorrhoeae MS11

51.306

94.7

0.486
  pilB Vibrio campbellii strain DS40M4

53.62

90.283

0.484